Method for preparing nucleotide yeast through enzymolysis
A nucleotide and yeast technology, applied in the fields of biological feed and biochemical industry, can solve the problems of low free nucleotide content, low amino acid nitrogen, poor water solubility, etc., and achieve good water solubility, mild conditions, and low energy consumption. Effect
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Embodiment 1
[0038] Activate the high nucleic acid yeast strain (Candida utilis, the strain number is TKZY-05, the preservation number is CGMCC No.7521, and the mass content of ribonucleic acid in the thalline is 17.3% of the dry weight of the thalline) Insert shake flask culture medium after (the concentration of carbon source is 50g / L, the concentration of nitrogen source is 4g / L, the concentration of phosphoric acid is 3mL / L, the concentration of zinc sulfate and ferrous sulfate is 0.1g / L, solvent water), and after cultivating for 10 hours, put it into the primary seed tank (1m 3 tank), after 8 hours of cultivation, it was connected to the secondary seed tank (10m 3 tank), and after 6 hours of cultivation, it was connected to a fermenter (100m 3 tank, filling capacity 60%), cultured to the middle stage of the yeast logarithmic growth phase, when the dry yeast concentration is 23g / L, it starts to flow out the fermentation liquid while supplementing the medium, and the flow rate of the f...
Embodiment 2
[0041] The fermentation process is the same as in Example 1.
[0042] Concentrate the fermented liquid through a yeast separator, collect 15t of yeast dense phase (concentrated phase dry matter content 16.5%, ribonucleic acid mass content in the thalline 18.2%), add 1t of water to prepare yeast liquid, pour into 20m 3 Broken kettle. Raise the temperature to 60±1°C, add 0.3% (w / w) of yeast dry weight, that is, 7.2kg papain, the enzyme activity is 600,000 U / g, and keep stirring for 10 hours for enzymolysis. Raise the temperature to 65±1°C, adjust the pH to 5.5-5.6, add 3000U / gRNA nuclease solution (enzyme solution volume (m 3 ) The specific calculation method is: the quality of yeast dense phase (15t)*concentrated dry matter content (16.5%)*ribonucleic acid quality content in the bacteria (18.2%)*3000 / 2000), the enzyme activity is 2000U / mL, maintain 65 Enzymolysis was stirred at ±1°C for 7 hours. Blowing is carried out and spray-dried, and the high-nucleotide yeast product is...
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