Method for preparing nucleotide yeast through enzymolysis

A nucleotide and yeast technology, applied in the fields of biological feed and biochemical industry, can solve the problems of low free nucleotide content, low amino acid nitrogen, poor water solubility, etc., and achieve good water solubility, mild conditions, and low energy consumption. Effect

Active Publication Date: 2019-09-10
南阳同凯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similar products currently on the market either have low free nucleotide content (2% to 5%), or low amino acid nitrogen, and poor water solubility affects animal digestion and absorption. There is an urgent need to develop a high free nucleotide content, amino acid nitrogen Suitable and water-soluble yeast products for feed enterprises to adopt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Activate the high nucleic acid yeast strain (Candida utilis, the strain number is TKZY-05, the preservation number is CGMCC No.7521, and the mass content of ribonucleic acid in the thalline is 17.3% of the dry weight of the thalline) Insert shake flask culture medium after (the concentration of carbon source is 50g / L, the concentration of nitrogen source is 4g / L, the concentration of phosphoric acid is 3mL / L, the concentration of zinc sulfate and ferrous sulfate is 0.1g / L, solvent water), and after cultivating for 10 hours, put it into the primary seed tank (1m 3 tank), after 8 hours of cultivation, it was connected to the secondary seed tank (10m 3 tank), and after 6 hours of cultivation, it was connected to a fermenter (100m 3 tank, filling capacity 60%), cultured to the middle stage of the yeast logarithmic growth phase, when the dry yeast concentration is 23g / L, it starts to flow out the fermentation liquid while supplementing the medium, and the flow rate of the f...

Embodiment 2

[0041] The fermentation process is the same as in Example 1.

[0042] Concentrate the fermented liquid through a yeast separator, collect 15t of yeast dense phase (concentrated phase dry matter content 16.5%, ribonucleic acid mass content in the thalline 18.2%), add 1t of water to prepare yeast liquid, pour into 20m 3 Broken kettle. Raise the temperature to 60±1°C, add 0.3% (w / w) of yeast dry weight, that is, 7.2kg papain, the enzyme activity is 600,000 U / g, and keep stirring for 10 hours for enzymolysis. Raise the temperature to 65±1°C, adjust the pH to 5.5-5.6, add 3000U / gRNA nuclease solution (enzyme solution volume (m 3 ) The specific calculation method is: the quality of yeast dense phase (15t)*concentrated dry matter content (16.5%)*ribonucleic acid quality content in the bacteria (18.2%)*3000 / 2000), the enzyme activity is 2000U / mL, maintain 65 Enzymolysis was stirred at ±1°C for 7 hours. Blowing is carried out and spray-dried, and the high-nucleotide yeast product is...

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PUM

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Abstract

The invention discloses a method for preparing nucleotide yeast through enzymolysis, and belongs to the field of biochemical engineering and biological feeds. The method specifically includes the steps of conducting continuous fermenting to obtain fermentation broth with yeast thalli, separating out the yeast thalli, conducting protease enzymolysis so that ribonucleic acid can dissociate outside cells, adding nuclease for oriented enzymolysis, and drying enzymolysis liquid to prepare the nucleotide yeast product. The method is simple and easy to implement, and the content of free nucleotides in the prepared yeast product can reach 11% or above and is 2-4 times that of domestic and international similar products (the content of free nucleotides in the similar products is 2-5%); in addition,the content of amino acid nitrogen in the product is 3% or above, and the product is high in water dissolubility and has the dissolubility of 90% or above. The product is delicious in taste and has the extremely good food calling performance and healthcare effect on livestock and domestic animals.

Description

technical field [0001] The invention belongs to the fields of biochemical industry and biological feed, and in particular relates to a method for preparing nucleotide yeast by enzymatic hydrolysis. Background technique [0002] Single-cell protein products are rich in nucleotides, and yeast products on the market are the main nucleotide providers for the feed industry. Due to the reasons of the yeast itself and the characteristics of the process, products such as autolyzed yeast, enzymatic yeast, and hydrolyzed yeast contain free nucleic acid hydrolysates, including free nucleotides, oligonucleotides, polynucleotides, bases, etc. wait. The free nucleotides in it can be quickly absorbed by animals and play important physiological functions. Other nucleotides have the problem of secondary degradation before they can be absorbed, and the bases will cause adverse effects on the liver and kidneys of animals. Therefore, the proportion of free nucleotides is related to the value ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12N1/16C12P19/30C12P19/32A23K20/153C12R1/72
CPCC12N1/06C12N1/16C12P19/30C12P19/32A23K20/153Y02P60/87
Inventor 黄小权陈文军芦强付天松刘志勇
Owner 南阳同凯生物技术有限公司
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