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A kind of extraction method of magnetic particle in honeybee

An extraction method and magnetic particle technology are applied in the field of extraction of magnetic particles in honeybees, which can solve the problems of low extraction rate of magnetic particles and complicated operation, and achieve the effects of overcoming long extraction period, improving recovery rate and high saturation magnetization.

Active Publication Date: 2021-06-11
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, there is no report on the purification method of the magnetic particle extractant in honeybees, and the present invention elaborates it in detail, effectively improving the problems of low magnetic particle extraction rate and complicated operation in the prior art, and for the final realization of magnetic particle extraction. The large number of applications of particles is of great significance

Method used

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  • A kind of extraction method of magnetic particle in honeybee
  • A kind of extraction method of magnetic particle in honeybee
  • A kind of extraction method of magnetic particle in honeybee

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1) Honeybee pretreatment: the honeybee is washed with 80% (v / v) ethanol solution and then dried, the head and chest are removed, and the abdomen is taken.

[0049] 2) Liquid nitrogen grinding treatment: the bee abdomen obtained in step 1) was placed in a mortar and continuously added with liquid nitrogen, and ground for 4-6 minutes until it was completely powdered.

[0050] 3) Sodium hypochlorite solution treatment: put the honeybee abdomen powder obtained in step 2) and the sodium hypochlorite solution containing PMSF at a mass ratio of 1:2 in a 2ml centrifuge tube, and soak at 4°C for 48h. In the sodium hypochlorite solution, the mass fraction of sodium hypochlorite is 4.2%. The concentration of PMSF was 8 mg / L.

[0051] 4) Extraction of magnetic particles by high-speed centrifugation: centrifuge the honeybee abdomen powder treated in 3) at a speed of 7000rpm for 6min, pour off the supernatant, and add a sodium hypochlorite solution with a mass fraction of 4.2% (whic...

Embodiment 2

[0055] 1) Honeybee pretreatment: the honeybee is washed with 80% (v / v) ethanol solution and then dried, the head and chest are removed, and the abdomen is taken.

[0056] 2) Liquid nitrogen grinding treatment: the bee abdomen obtained in step 1) was placed in a mortar and continuously added with liquid nitrogen, and ground for 4-6 minutes until it was completely powdered.

[0057] 3) Sodium hypochlorite solution treatment: put the honeybee belly powder obtained in step 2) and the sodium hypochlorite solution containing PMSF at a mass ratio of 1:2 in a 2ml centrifuge tube, soak at 4°C for 72h. In the sodium hypochlorite solution, the mass fraction of sodium hypochlorite is 6.3%, and the concentration of PMSF is 8 mg / L.

[0058] 4) Extraction of magnetic particles by high-speed centrifugation: Centrifuge the honeybee abdomen powder treated in 3) at a speed of 9000rpm for 8min, pour off the supernatant, and add a sodium hypochlorite solution with a mass fraction of 6.3% (which co...

Embodiment 3

[0062] 1) Honeybee pretreatment: the honeybee is washed with 80% (v / v) ethanol solution and then dried, the head and chest are removed, and the abdomen is taken.

[0063] 2) Liquid nitrogen grinding treatment: the bee abdomen obtained in step 1) was placed in a mortar and continuously added with liquid nitrogen, and ground for 4-6 minutes until it was completely powdered.

[0064] 3) Sodium hypochlorite solution treatment: the bee belly powder obtained in step 2) and the sodium hypochlorite solution containing PMSF were placed in a 2mL centrifuge tube at a mass ratio of 1:2, and soaked at 4°C for 96h. In the sodium hypochlorite solution, the mass fraction of sodium hypochlorite is 8.4%, and the concentration of PMSF is 8 mg / L.

[0065] 4) Extraction of magnetic particles by high-speed centrifugation: Centrifuge the honeybee abdomen powder treated in 3) at a speed of 10000rpm for 10min, pour off the supernatant, and add a sodium hypochlorite solution with a mass fraction of 8.4...

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Abstract

The invention relates to a method for extracting magnetic particles from honeybees, which comprises the following steps: treating honeybee abdomen powder with sodium hypochlorite solution containing PMSF, centrifuging, and magnetically adsorbing to obtain magnetic particles. The magnetic particles separated by the invention have the characteristics of uniform particle size, good dispersibility, high purity, strong magnetism, high extraction rate and the like. The method of the invention has the advantages of easy acquisition of experimental raw materials, simple implementation process, low equipment requirements, low cost, and environmental protection.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for extracting magnetic particles from honeybees. Background technique [0002] Magnetic particles have excellent physical and chemical properties such as good magnetic properties, large specific surface area, and easy surface functionalization. It can be artificially synthesized by physical or chemical methods, but its size range is widely distributed, there is no biofilm on the surface, it is easy to aggregate, and it loses the unique properties of magnetic materials. The magnetic particles synthesized in the body are mineralized in the vesicles of the body, and there is a biofilm on the surface, which makes it have good biocompatibility, pure composition, high magnetism, good dispersion, and unique crystal form. features. Because it is not easy to aggregate, has no cytotoxicity, has a huge specific surface area, uniform particles and its unique biological characteristic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01F1/01B03C1/00B04B5/00
CPCB03C1/00B04B5/00H01F1/01
Inventor 尚锐张爽张国静张雪莹王事成
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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