Prenylated flavonoid compound apricotone u and its preparation method and application
A technology of prenylated flavonoids and peach seven, applied in the field of medicine
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Embodiment 1
[0016] The method of preparing an isoprene-based flavonoid compound peaches in the present invention is that the laheline drug is 9kg as a raw material, with 18L, volume ratio of 95% ethanol, heat reflow, extraction temperature is 95 ° C Each extraction time is 1.5 hours, and ethanol is recovered under reduced pressure, and the paste-shaped ethanol extract is reduced, and the transmissyle ethanol extract is reduced, and the oil ether, dichloromethane, ethyl acetate, n-butanol is extracted 3 times. Each time 3.2L, the time is 1.5 hours; the ethyl acetate extraction site is initially separated by silicate chromatography, and the volume ratio is 100: 0,100: 5,100: 7,100: 10,100: 30,100 : 50, 100: 70, 100: 100, 100: 200, 0: 100 Petroleum ether-acetone mixed solvent system for gradient elution, each gradient is eluent with 13 L / min, and the flow rate is 15 mL / min, each 500ml volume is One-phase, 260 copies, each of the circulatory analysis of silica gel, using GF254 thin laminate, ...
Embodiment 2
[0018]The method of preparing the isoproprene U.Strate, for example, the lanedine drug 6kg is a raw material, and the volume ratio is 75% ethanol heating and reflows for 3 times, the extraction temperature is 90 ° C. Each extraction time is 2 hours, and ethanol is recovered under reduced pressure, so that the paste-shaped ethanol extract is reduced, and in the distilled water of 2L, it is sequentially extracted from petroleum ether, dichloromethane, ethyl acetate, and n-butanol. 3 times. Each time 2L, the time is 2 hours; the ethyl acetate extraction site is initially separated by silicate chromatography, and the volume ratio is 100: 0,100: 5,100: 7,100: 10,100: 30,100: 50 100: 70, 100: 100, 100: 200, 0: 100 of the petroleum ether-acetone mixed solvent system for gradient elution, each gradient with 9.1l eluent, flow rate of 10 mL / min, each 350ml volume is first class Add 260 copies, each of the circular silica gel is chromatographic detection, using GF254 thin laminate, respect...
Embodiment 3
[0020] The preparation method of an isoprene-based flavonoids in the present invention is that 8 kg of the leaflets is 8kg as a raw material, and the volume ratio is 85% ethanol, and the extraction temperature is 92 ° C. Each extraction time is 1.5 hours, and ethanol is recovered under reduced pressure, and the paste-shaped ethanol extract is reduced, and the transmissions of the paste et al., It is sequentially extracted from petroleum ether, dichloromethane, ethyl acetate, n-butanol. 3 times. Each time 2.8L, the time is 1.5 hours; the ethyl acetate extraction site is initially separated by silica gel column chromatography, with a volume ratio of 100: 0,100: 5,100: 7,100: 10,100: 30,100 : 50, 100: 70, 100: 100, 100: 200, 0: 100 of the petroleum ether-acetone mixed solvent system for gradient elution, each gradient is eluent with 11.7L / min, the flow rate is 13mL / min, each 450ml volume For one-phase, 260 copies, each of the circulatures were analyzed by silica gel, using GF254 ...
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