Preparation method and application of extract solution of whole plants of cucumis metuliferus
The technology of Langya fruit and extract is applied in the field of preparation of the whole extract of Langya fruit, can solve the problems of neglect of nutrition and the like, and achieve the effects of improving utilization rate, promoting intestinal digestion and absorption, and improving therapeutic effect.
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Embodiment 1
[0035] A preparation method of wolfya whole plant extract, comprising the steps of:
[0036] 1) Preprocessing:
[0037] (11) fruit, root, stem and leaf of Wolf's Tooth are cleaned;
[0038] (12) Mix the cleaned roots, stems and leaves in the step (11), and dry them in an oven at 50° C. to obtain the raw material of the wolf tooth fruit plant, which is set aside.
[0039] 2) Beating: first mix according to the ratio of water: wolfya fruit (m / m) = 2:1 to obtain a mixed system; then place the mixed system in a beater for beating to obtain a wolfya fruit stock solution;
[0040] 3) Centrifugation: centrifuge the wolfya fruit stock solution obtained in step 2) at 4° C. and 12000 r / min for 5 minutes, and take the supernatant to obtain the supernatant of the fruit stock solution;
[0041] 4) Filtration: filtering the supernatant of the fruit stock solution obtained in step 3) to obtain a fruit filtrate;
[0042] 5) Reflux extraction: add 3 times the volume of absolute ethanol to t...
Embodiment 2
[0050] A preparation method of wolfya whole plant extract, comprising the steps of:
[0051] 1) Preprocessing:
[0052] (11) fruit, root, stem and leaf of Wolf's Tooth are cleaned;
[0053] (12) Mix the cleaned roots, stems and leaves in step (11), and dry them in an oven at 55° C. to obtain the raw material of wolf tooth fruit plant, which is set aside.
[0054] 2) Beating: first mix according to the ratio of water: wolfya fruit (m / m) = 3:1 to obtain a mixed system; then place the mixed system in a beater for beating to obtain wolfya fruit stock solution;
[0055] 3) Centrifugation: centrifuge the wolfya fruit stock solution obtained in step 2) at 4°C and 13000r / min for 7min, take the supernatant, and obtain the supernatant of the fruit stock solution;
[0056] 4) Filtration: filtering the supernatant of the fruit stock solution obtained in step 3) to obtain a fruit filtrate;
[0057]5) Reflux extraction: Add 4 times the volume of absolute ethanol to the fruit filtrate obt...
Embodiment 3
[0065] A preparation method of wolfya whole plant extract, comprising the steps of:
[0066] 1) Preprocessing:
[0067] (11) fruit, root, stem and leaf of Wolf's Tooth are cleaned;
[0068] (12) Mix the cleaned roots, stems and leaves in the step (11), and place them in an oven at 60° C. to dry to obtain the raw material of the wolf tooth fruit plant, which is set aside.
[0069] 2) Beating: first mix according to the ratio of water: wolfya fruit (m / m) = 3.5:1 to obtain a mixed system; then place the mixed system in a beater for beating to obtain wolfya fruit stock solution;
[0070] 3) Centrifugation: Centrifuge the wolfya fruit stock solution obtained in step 2) at 4°C and 13000r / min for 8min, take the supernatant, and obtain the supernatant of the fruit stock solution;
[0071] 4) Filtration: filtering the supernatant of the fruit stock solution obtained in step 3) to obtain a fruit filtrate;
[0072] 5) Reflux extraction: Add 4 times the volume of absolute ethanol to th...
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