Pseudomonas corrugata and its application

A technology of Pseudomonas rugosa and strains, applied in the field of microorganisms, can solve problems such as destruction, unsatisfactory results, and pollution

Active Publication Date: 2019-11-05
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of ideal disease-resistant tobacco cultivars in production, at present, the control of tobacco black shank and bacterial wilt still relies on chemical agents, but the effects are not ideal, and there are many disadvantages
For example, the currently used chemical fungicides pollute or destroy soil microorganisms, plants, water sources and the atmosphere, and the problem of drug resistance is becoming increasingly prominent, and drug residues in plants will indirectly cause harm to humans

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pseudomonas corrugata and its application
  • Pseudomonas corrugata and its application
  • Pseudomonas corrugata and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Example 1. Pseudomonas crimpingans ( Pseudomonas corrugata ) Isolation and identification of S58 CGMCC No.17043

[0153] 1. Pseudomonas wrinkles ( Pseudomonas corrugata ) Separation of S58 CGMCC No.17043

[0154] Pseudomonas crinkle ( Pseudomonas corrugata ) S58 CGMCC No.17043 is obtained from tobacco root girth. Tobacco rhizome samples were collected from Yanhe Town, Yuxi City, Yunnan Province. Rootsoil samples were brought back to the laboratory for cryopreservation. 1g of the soil samples were suspended in 9ml of sterile water. After full shaking, they were serially diluted and spread on NA medium plates. Cultivate for 48h at ℃. Pick a single colony and streak it on the plate again for purification and preservation. Among them, the vigorous ones are designated as S58 strain, which is the Pseudomonas corrugae ( Pseudomonas corrugata ) S58 CGMCC No.17043.

[0155] 2. Pseudomonas crinkle ( Pseudomonas corrugata ) Identification of S58 CGMCC No.17043

[0156] The above-me...

Embodiment 2

[0160] Example 2. Pseudomonas crimpingans ( Pseudomonas corrugata ) Determination of the antibacterial spectrum of S58 CGMCC No.17043

[0161] The tested plant pathogenic fungi: Phytophthora nicotianae, Phytophthora sojae, Rhizoctonia solani, Magnaporthe grisea.

[0162] Tested plant pathogenic bacteria: Acidobacillus watermelon, Corynebacterium michigan subsp. michigan, Xanthomonas oryzae, R. solanacearum and Ralstonia solanacearum.

[0163] Antibacterial experiments were carried out on the above-mentioned tested plant pathogenic fungi using the plate confrontation culture method. The specific operation is as follows: Pseudomonas rugosa S58 was cultured on LB solid medium at 28°C for 48 hours. The above-mentioned test plant pathogenic fungi were cultured on a PDA plate at a constant temperature of 25°C-28°C for 3-4 days, and agar plate with pathogenic bacteria was made from the edge of the colony with a sterile stainless steel puncher with a diameter of 0.7cm; picked with a steril...

Embodiment 3

[0166] Example 3. The control experiment of the bacterial agent containing Pseudomonas rugosus S58 on tobacco bacterial wilt

[0167] 1. Preparation of pathogenic inoculum: Ralstonia solanacearum-Ralstonia solanacearum was streaked on NA medium, cultured upside down at 28°C for 3 days, and set aside.

[0168] 2. Preparation of liquid inoculum containing Pseudomonas clavulae S58:

[0169] The preparation of the liquid inoculum containing Pseudomonas rugosus S58 includes the steps of slope cultivation, seed culture, fermentation and inoculum preparation, specifically including:

[0170] A. Inclined surface culture: Inoculate Pseudomonas rugosus S58 on the inclined surface medium-nutrient agar medium, and cultivate for 48 hours at a temperature of 28-30℃ to obtain the inclined surface strain;

[0171] B. Seed culture: inoculate the slant strains into a liquid medium, and cultivate at a temperature of 29-33°C for 18-24 hours to obtain liquid fermented seeds; the liquid medium is beef extrac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses Pseudomonas corrugata and an application thereof. A strain number of Pseudomonas corrugata is S58, and a registration number is CGMCC No.17043 in the General Microbiology Center of the China Microbial Culture Collection Management Committee. The strain has stable perforamnce, high-efficiency, broad-spectrum antibacterial properties, and has the inhibitory effects for phytopathogenic fungi such as phytophthora nicotianae, phytophthora sojae, rhizoctonia solani and Magnaporthe oryzae, and acidovorax citrulli, Clavibacter, and flavimonas oryzihabitans and ralstonia solanacearum. The control effect of Pseudomonas corrugata S58 on ralstonia solanacearum is 76.2%, and the control effect against tobacco black shank is 60.5%. The Pseudomonas corrugata S58 is capable of producing ferritin and amylase, can cause programmed cell death in the surface immune response of plants, and can control the occurrence of tobacco wildfire by stimulating immune resistance. The Pseudomonas corrugata S58 is capable of hydrolyzing organic phosphorus and can be used for soil improvement.

Description

Technical field [0001] The invention relates to a strain of Pseudomonas rugosus in the field of microorganisms and its application. Background technique [0002] Tobacco is an economic plant in China. With the development of tobacco production, the area of ​​continuous cropping has increased, and the occurrence of diseases has become more and more serious, which has become one of the main limiting factors to tobacco production. Tobacco black shank and bacterial wilt are the main and common soil-borne diseases of tobacco, which can be infected from the seedling stage to the adult plant. Such diseases are devastating diseases of tobacco. In recent years, due to the expansion of continuous cropping tobacco fields and the increase of continuous cropping years, tobacco black shank and bacterial wilt have become more prevalent in many places. Due to the lack of ideal disease-resistant tobacco cultivars in production, the prevention and control of tobacco black shank and bacterial wil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02C09K17/14A01P3/00A01P1/00A01P21/00C05F11/08C12R1/38
CPCA01N63/00C09K17/14C12N1/20C12N1/205C12R2001/38
Inventor 魏海雷谷医林张宏越
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap