Test method for stearoyl lactylate in flour or flour improver
A technology of stearoyl lactate and flour improver, applied in the field of analytical chemistry, can solve problems such as interference accuracy, and achieve the effects of sensitive response, good chromatographic peak shape and retention time, and low detection limit and quantification limit
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preparation example Construction
[0038] Preferably, the preparation method of the standard solution of the series concentration comprises: accurately weighing the sodium stearoyl lactylate standard, dissolving it with acetone, and diluting it with acetonitrile to obtain a standard stock solution; The product stock solution was diluted and fixed to volume to obtain a series of standard product solutions.
[0039] The present invention also provides the application of the above stearoyl lactylate detection method in the qualitative or quantitative detection of stearoyl lactylate in food.
[0040] In this application, optionally, the food includes: flour, flour improver, wheat flour or wheat flour improver.
[0041] The reagent specific information used in the embodiment of the present invention is as follows:
[0042] Standard stearoyl lactylate: the purity is greater than 99%, purchased from Alfa Aesar;
[0043] Wheat flour: purchased from the local market;
[0044] Flour improver: including but not limited...
Embodiment 1
[0045] The mensuration of embodiment 1 stearoyl lactylate standard solution
[0046]1) Preparation of stearoyl lactylate standard solution: Weigh an appropriate amount of stearoyl lactylate standard product, dissolve it in acetone, and dilute to 10 mL with acetonitrile to prepare stearoyl lactylate with a concentration of 500 mg / L (in terms of stearoyl lactylic acid hydrochloride concentration) standard stock solution. Draw 20 μL of the stock solution into a 10mL volumetric product, and the volume has been adjusted to 10mL to prepare a stearoyl lactylate standard solution with a concentration of 1mg / kg.
[0047] 2) Chromatographic conditions
[0048] Chromatographic column: HILIC chromatographic column (50mm×4.6mm×2.6μm)
[0049] Column temperature 40°C;
[0050] Injection volume: 1.0μL;
[0051] Flow rate: 0.2mL / min;
[0052] Mobile phase: A is 0.1% ammonia water, B is acetonitrile; mobile phase ratio A:B=5:95.
[0053] 3) Mass Spectrometry Conditions
[0054] Ion sour...
Embodiment 2
[0061] Determination of stearoyl lactylate content in embodiment 2 negative sample (wheat flour sample not containing stearoyl lactylate)
[0062] 1) Preparation of sample solution
[0063] Weigh 1.0g of wheat flour sample not containing stearoyl lactylate (negative sample) into a 15mL stoppered plastic centrifuge tube, add 5.0mL of acetonitrile solution to extract the analyte in the sample, vortex for 2min , ultrasonic extraction for 10 min, and after standing still, take 1 mL of the supernatant and centrifuge at 15,000 r / min for 5 min. Aspirate the supernatant for determination.
[0064] 2) Chromatographic conditions
[0065] Same as implementation example 1.
[0066] 3) Mass Spectrometry Conditions
[0067] Same as implementation example 1.
[0068] 4) The chromatogram of the negative sample is as follows figure 2 As shown, it can be seen that because stearoyl lactylate is not contained in the negative sample, there are no peaks in the chromatogram.
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Abstract
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