Preservation solution and preservation method of sheep semen

A preservation method and technology of preservation solution, which are applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of restricting the promotion of preservation solution of sheep semen, high requirements for cold storage and preservation conditions, and low success rate of artificial insemination, etc. To achieve the effect of no need for special temperature control and refrigeration equipment, to improve the conception rate during estrus, and to facilitate popularization and application

Active Publication Date: 2021-07-27
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few types of sheep semen preservation solutions, and the requirements for cold storage conditions are high, and the cost is high, which limits the widespread promotion of sheep semen preservation solutions
However, under normal temperature or low temperature storage conditions, the sperm metabolism is fast, the storage time is short, the sperm survival rate after storage is low, and the success rate of artificial insemination is low.

Method used

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  • Preservation solution and preservation method of sheep semen
  • Preservation solution and preservation method of sheep semen
  • Preservation solution and preservation method of sheep semen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A sheep semen preservation solution, the preservation solution includes the following components per 100mL: sodium salicylate, vitamin E 4mL, fresh egg yolk 20mL, dimethyl sulfoxide 2mL, fructose 1.26g, citric acid monohydrate 1.72g, three 3.53g of hydroxymethylaminomethane, 0.5g of vitamin C, 0.5g of bovine serum albumin, 15000IU of penicillin sodium, 15000IU of streptomycin sulfate, and double distilled water as the balance. Prepare 5 groups of dilutions, group 1: blank control, sodium salicylate 0g; group 2: sodium salicylate 0.1601g / 100mL (corresponding to 10mM in the table); group 3: sodium salicylate 0.3202g / 100mL (corresponding to table in 20mM); group 4: sodium salicylate 0.8005g / 100mL (corresponding to 50mM in the table); group 5: sodium salicylate 1.6010g / 100mL (corresponding to 100mM in the table).

Embodiment 2

[0041] A sheep semen preservation solution, the preservation solution includes the following components per 100mL: sodium salicylate, vitamin E 4mL, fresh egg yolk 20mL, glycerin 4mL, fructose 1.26g, citric acid monohydrate 1.72g, trishydroxymethyl amino Methane 3.53g, vitamin C 0.5g, bovine serum albumin 0.5g, penicillin sodium 15000IU, streptomycin sulfate 15000IU, the balance is double distilled water. Prepare 5 groups of dilutions, group 1: sodium salicylate 0g; group 2: sodium salicylate 0.1601g / 100mL (corresponding to 10mM in the table); group 3: sodium salicylate 0.3202g / 100mL (corresponding to 20mM in the table) ; Group 4: sodium salicylate 0.8005g / 100mL (corresponding to 50mM in the table); Group 5: sodium salicylate 1.6010g / 100mL (corresponding to 100mM in the table).

Embodiment 3

[0043] A sheep semen preservation solution, the preservation solution includes the following components per 100mL: acetylsalicylic acid, vitamin E 4mL, fresh egg yolk 20mL, dimethyl sulfoxide 2mL, fructose 1.26g, citric acid monohydrate 1.72g, three 3.53g of hydroxymethylaminomethane, 0.5g of vitamin C, 0.5g of bovine serum albumin, 15000IU of penicillin sodium, 15000IU of streptomycin sulfate, and double distilled water as the balance. Prepare 5 groups of preservation solutions Group 1: acetylsalicylic acid 0g; Group 2: acetylsalicylic acid 0.1601g / 100mL (corresponding to 10mM in the table); Group 3: acetylsalicylic acid 0.3202g / 100mL (corresponding to 20mM in the table); Group 4: acetylsalicylic acid 0.8005g / 100mL (corresponding to 50mM in the table); Group 5: acetylsalicylic acid 1.6010g / 100mL (corresponding to 100mM in the table).

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Abstract

The invention relates to a preservation solution and a preservation method of goat semen. Each 100mL of this preservation solution includes components: 0.16-1.6mg sodium salicylate or 0.18-1.8mg acetylsalicylic acid, 3.5-4.5mL vitamin E, 19-21mL fresh egg yolk, 1.5-2.5mL dimethyl sulfoxide Or glycerin 3.5-4.5mL, fructose 1.2-1.3g, citric acid monohydrate 1.7-1.8g, trishydroxymethylaminomethane 3.5-3.6g, vitamin C 0.4-0.6g, bovine serum albumin 0.4-0.6g, Penicillin sodium 10000~15000IU, streptomycin sulfate 10000~15000IU, the balance is double distilled water. The invention effectively prolongs the storage time of goat semen under normal temperature and low temperature conditions, and improves the quality of preserved sperm.

Description

technical field [0001] The invention relates to a semen preservation solution, in particular to a sheep semen preservation solution and a preservation method. Background technique [0002] my country has a long history of sheep breeding, and the germplasm resources of sheep and goats are abundant, among which the number of breeding accounts for the first in the world. The sheep raising industry is of great significance to the modernization of the country. The sheep raising industry has great development prospects in the vast grasslands and rural areas. The rapid development of the sheep raising industry can not only contribute to the country's reform and opening up, but also improve people's living standards. With the continuous advancement of science and technology, my country's sheep breeding industry has also made great progress, but there are still many problems. Compared with developed countries, there is still a big gap, especially in terms of large-scale breeding and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 曹鸿国李彩霞唐云书孙寒雪高晋凤涂丹凤卢振涛张苍龙孟祝张子军任春环陈家宏刘亚周建军陈宏权
Owner ANHUI AGRICULTURAL UNIVERSITY
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