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Alkalophilic and aerobic denitrifying bacteria and application thereof

A technology of denitrifying bacteria and alkaliphiles, applied in the field of environmental microorganisms, can solve the problems of low nitrate tolerance, low nitrate tolerance, and low denitrification efficiency, and achieve fast, effective and durable denitrification. The effect of high acceptability and strong denitrification ability

Active Publication Date: 2019-11-26
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The same denitrification rate is low, and the tolerance to nitrate is not high, only 20mg / L
[0007] In general, most of the reported denitrifying bacteria have problems such as low tolerance to nitrate or low denitrification efficiency, which are subject to certain restrictions in practical application.

Method used

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  • Alkalophilic and aerobic denitrifying bacteria and application thereof
  • Alkalophilic and aerobic denitrifying bacteria and application thereof
  • Alkalophilic and aerobic denitrifying bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Isolation and identification of facultative anaerobic denitrifying strain (Pseudomonas stutzeri HK13)

[0042] 1.1 Experimental materials

[0043] 1.1.1 Source of strain

[0044] The strain was isolated from activated sludge in a denitrification anoxic tank of coal gasification wastewater in Hubei.

[0045] 1.1.2 Medium

[0046] Denitrification medium (g L -1 ): Trisodium citrate 5; KH 2 PO 4 1;K 2 HPO 4 1; KNO 3 2; MgSO 4 ·7H 2 O0.2; pH 7.0 ~ 7.2;

[0047] Bromothymol blue medium (BTB) (g L -1 ): L-Asparagine 1; Trisodium Citrate 2; KNO 3 1; KH 2 PO 4 1; FeCl 2 4H 2 O 0.05; CaCl 2 0.15; MgSO 4 ·7H 2 O 1; BTB (1% ethanol solution) 1 mL; Agar 16; pH 7.0;

[0048] LB activation preservation medium (g L -1 ): peptone 10; yeast powder 5; NaCl 10; all media were sterilized at 121°C for 20 minutes.

[0049] 1.2 Experimental method

[0050] The activated sludge collected from a coal gasification wastewater anoxic pool in Hubei was precipitated to m...

Embodiment 2

[0059] Optimization of Growth Conditions for Pseudomonas HK13

[0060] (1) Research on the optimal C / N ratio of the bacterial strain HK13 obtained in Example 1

[0061] In this embodiment, the initial nitrate nitrogen concentration is controlled to be 100mg / L, the pH is 7.0, and the C / N is 2, 4, 6, 8, and 10 respectively to prepare corresponding denitrification medium, and inoculate the bacterial solution with a volume fraction of 5%. (The OD of bacterial solution was enriched and activated by LB medium 600 =1), placed in 30°C, 100rpm constant temperature shaking incubator for 24h, sampling, high-speed centrifugation (12000rpm, 4°C, 5min), after 0.22μm filter membrane filtration, measure the NO of the filtrate 3 - -N and NO 2 - -N concentration. A blank control and parallel samples were set up in the experiment, and each experiment was repeated twice.

[0062] image 3 Shows the NO of strain HK13 cultured under different C / N conditions x - -N removal rate, the bacteri...

Embodiment 3

[0073] Tolerance of Strain HK13 to Nitrate Nitrogen

[0074] This example explores the ability of strain HK13 to treat high-concentration nitrate wastewater. During the experiment, C / N=8, pH=8, and initial NO 3 - -N concentration control is 450mg / L to prepare the corresponding denitrification medium. Inoculate the bacterial solution with a volume fraction of 5% (enriched and activated to the OD of the bacterial solution through LB medium 600 =1), placed in a constant temperature shaking incubator at 35°C, 100rpm, and cultured for 3 days, samples were taken every 8h, centrifuged at a high speed (12000rpm, 4°C, 5min), filtered through a 0.22μm filter membrane, and the NO of the filtrate was measured 3 - -N and NO 2 - -N concentration. A blank control and parallel samples were set up in the experiment, and each experiment was repeated twice. The measurement results are shown in Table 1.

[0075] Table 1

[0076]

[0077] It can be seen from Table 1 that the nitrate ni...

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Abstract

The present invention relates to a strain of alkalophilic and aerobic denitrifying bacteria and application thereof. The strain is named as pseudomonas HK13 and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO:M2019611, the preservation date is August 7, 2019, the preservation address is Wuhan University, No.299, Bayi Road, Wuchang District, Wuhan City,Hubei Province, China. Under the optimal growth conditions, the strain KK13 has a total nitrogen removal rate of 92.62% in 12 h and an average nitrogen removal rate of 8.26 mg (L.h)<-1>, and denitrification speed is high and denitrification is effective. The adaptive PH range of the strain is6.5-10. In a strong alkali environment with pH being 10, the removal rate of total nitrogen oxide by the strain is more than 98% in 16h, and it is indicated that the strain has alkali resistance and can be used for treatment of nitrate-containing alkaline wastewater. The strain has high tolerance to bothnitrate nitrogen and nitrite nitrogen, is suitable for wastewater containing nitrate and nitrite with higher concentration, and is wide in application prospects in the field of sewage treatment.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to an alkaliphilic aerobic denitrification bacterium and its application. Background technique [0002] With the rapid development of industry and agriculture, a large amount of untreated nitrogen-containing wastewater is discharged into rivers and lakes, causing more and more serious problems such as eutrophication of water bodies, endangering many industries such as agriculture, fishery, tourism, etc., and affecting drinking water sanitation And food safety also poses a huge threat. As the country pays more and more attention to environmental protection, the total nitrogen discharge standard of wastewater is becoming more and more stringent. However, the nitrogen content of the discharged water after the secondary treatment of sewage is still high, resulting in many industries failing to meet the discharge standards, and after the secondary biochem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
CPCC02F3/34C02F2101/163C02F2101/166C12N1/205C12R2001/38
Inventor 丁雷韩冰崔长征史沐袁明珠周建民薛佳佳
Owner EAST CHINA UNIV OF SCI & TECH
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