Method for constructing full-sib family of trachinotus ovatus

Abstract

The invention provides a method for constructing a full-l-sib family of trachinotus ovatus. The method comprises the following steps: (1) parent fish selection and breeding, wherein parent fishes of the trachinotus ovatus are screened out and marked by using electronic markers, and nutrient enrichment is carried out; (2) spawning induction and external marking of the parent fishes, wherein after the gonads of the parent fishes are mature, artificial spawning is carried out on the parent fishes by using an oxytocic, individual identification is carried out on the parent fishes by utilizing external markers, and corresponding electronic mark numbers are recorded; (3) parent fish observation and selection, wherein the mating behaviors of the parent fishes are observed after the oxytocic is injected, a pair of paired parent fishes is determined according to the mating behaviors of the parent fishes, the electronic marker numbers and the corresponding external markers of the individual paired parent fishes are recorded, and the paired parent fishes are selected out for independent breeding for spawning; (4) obtaining and breeding of fertilized ova, wherein fertilized ova are collected after spawning of the parent fishes, and fry breeding is carried out to obtain the full-sib family of the trachinotus ovatus. The method can effectively overcome the defect that female and male parentfishes of trachinotus ovatus are difficult to distinguish, and family breeding cannot be effectively carried out.

Description

technical field [0001] The invention relates to the technical field of breeding of ovate pomfret, in particular to a method for constructing a full-sib family of ovate pomfret. Background technique [0002] Family selection is one of the main means of breeding aquatic animals with excellent growth traits. In the world, large-scale family selection technology has been used to genetically improve aquaculture animals, and significant progress has been made. Norway used large-scale family selection technology to genetically improve Atlantic salmon and successfully bred high-quality Atlantic salmon strains, effectively avoiding the decline in economic traits during the breeding process, and economic traits have also been continuously improved. In addition, the large-scale family breeding technology was used to carry out the breeding of Indian carp, tilapia and rainbow trout, all of which achieved good breeding results. [0003] The pomfret (Trachinotus ovatus) is the main specie...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for constructing a family of full-sibling pomfrets, which solves the technical problems in the prior art that the male and female of the pomfrets are difficult to distinguish and the accuracy of family identification is insufficient.