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A method for measuring the osmotic fragility of red blood cells and its kit

A technology for measuring osmotic fragility and measuring method, applied in the field of red blood cell osmotic fragility measuring method and its kit, can solve the problems of difficult to guarantee repeatability, unable to reflect the whole process of hemolysis, and high time control requirements.

Active Publication Date: 2021-06-04
深圳褀氏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hemolysis rate at different time points is different. The rate method requires high time control, and the repeatability is difficult to guarantee.
In addition, the rate method cannot reflect the whole process of hemolysis

Method used

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  • A method for measuring the osmotic fragility of red blood cells and its kit
  • A method for measuring the osmotic fragility of red blood cells and its kit
  • A method for measuring the osmotic fragility of red blood cells and its kit

Examples

Experimental program
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Effect test

Embodiment 1

[0086] This embodiment provides a method for measuring the osmotic fragility of red blood cells, and the specific steps include:

[0087] S0, equipped with incomplete hemolysis hypotonic saline solution and complete hemolysis hypotonic saline solution.

[0088] Incomplete hemolysis hypotonic saline solution contains:

[0089] 4g / L sodium chloride

[0090] 0.1% (v / v) Proclin300

[0091] Completely hemolytic hypotonic saline solution contains:

[0092] 1g / L sodium chloride

[0093] 0.1% (v / v) Proclin300

[0094] S1. Add 1000uL of incomplete hemolytic hypotonic saline solution and complete hemolytic hypotonic saline solution into two cuvettes respectively;

[0095] S2. Add 6uL of whole blood to the above two cuvettes, mix well, start timing, and immediately use a scattering analyzer with a laser with a wavelength of 600nm as the light source to detect the scattering of the mixed solution in the incomplete hemolysis Light value A0, scattered light value B0 of the mixed solutio...

Embodiment 2

[0099] This embodiment provides a kit suitable for the above-mentioned method for measuring the osmotic fragility of red blood cells, including: a hypotonic saline solution with incomplete hemolysis and a hypotonic saline solution with complete hemolysis.

[0100] Incomplete hemolysis hypotonic saline solution contains:

[0101] 4g / L sodium chloride

[0102] 0.1% (v / v) Proclin300

[0103] Completely hemolytic hypotonic saline solution contains:

[0104] 1g / L sodium chloride

[0105] 0.1% (v / v) Proclin300.

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Abstract

The invention provides a method for measuring the osmotic fragility of red blood cells and a kit thereof, wherein the measuring method includes: S1, adding an equal amount of different concentrations of hypotonic saline solution into two cuvettes, one of which is incompletely dissolved The blood vessel, the hypotonic saline solution added to it cannot completely hemolyze the whole blood, and the other tube is completely hemolyzed, and the hypotonic saline solution added to it can completely hemolyze the whole blood; S2, put it into two cuvettes respectively Add an equal amount of whole blood, and after mixing, immediately detect the scattered light value A0 of the mixed solution in incomplete hemolysis, and the scattered light value B0 of the mixed solution in complete hemolysis; S3, at a specific time after the end of hemolysis, measure Scattered light value A1 of the mixed solution in incomplete hemolysis, B1 of the mixed solution in complete hemolysis; S4, according to the formula: percentage of hemolysis%=(A0‑A1) / (B0‑B1)×100% Percentage of blood vessel hemolysis. The detection result of the determination method is more accurate, and can realize standardization and consistency of results.

Description

technical field [0001] The invention relates to the technical field of measuring erythrocyte osmotic fragility, in particular to a method for measuring erythrocyte osmotic fragility and a kit thereof. Background technique [0002] The normal lifespan of mature red blood cells in the human body is about 120 days. During the survival period, they travel about 500 kilometers in the circulatory system and squeeze through blood vessels and pores that are much smaller than their own diameter many times. Therefore, red blood cells must have good toughness and deformability. Red blood cells mainly rely on the red blood cell membrane to maintain deformability and stability. If a certain protein on the membrane changes, it can cause hemolysis, such as hereditary spherocytosis. [0003] Erythrocyte osmotic fragility is a commonly used clinical index for testing red blood cell membrane defects. The erythrocyte osmotic fragility test is to measure the ability of red blood cells to withst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/51
CPCG01N21/51G01N2021/513
Inventor 廉正鑫祁晖
Owner 深圳褀氏生物科技有限公司
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