Unlock instant, AI-driven research and patent intelligence for your innovation.
A method for measuring the osmotic fragility of red blood cells and its kit
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for measuring osmotic fragility and measuring method, applied in the field of red blood cell osmotic fragility measuring method and its kit, can solve the problems of difficult to guarantee repeatability, unable to reflect the whole process of hemolysis, and high time control requirements.
Active Publication Date: 2021-06-04
深圳褀氏生物科技有限公司
View PDF7 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The hemolysis rate at different time points is different. The rate method requires high time control, and the repeatability is difficult to guarantee.
In addition, the rate method cannot reflect the whole process of hemolysis
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0086] This embodiment provides a method for measuring the osmotic fragility of red blood cells, and the specific steps include:
[0087] S0, equipped with incomplete hemolysis hypotonic saline solution and complete hemolysis hypotonic saline solution.
[0094] S1. Add 1000uL of incomplete hemolytic hypotonic saline solution and complete hemolytic hypotonic saline solution into two cuvettes respectively;
[0095] S2. Add 6uL of whole blood to the above two cuvettes, mix well, start timing, and immediately use a scattering analyzer with a laser with a wavelength of 600nm as the light source to detect the scattering of the mixed solution in the incomplete hemolysis Light value A0, scattered light value B0 of the mixed solutio...
Embodiment 2
[0099] This embodiment provides a kit suitable for the above-mentioned method for measuring the osmotic fragility of red blood cells, including: a hypotonic saline solution with incomplete hemolysis and a hypotonic saline solution with complete hemolysis.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention provides a method for measuring the osmotic fragility of red blood cells and a kit thereof, wherein the measuring method includes: S1, adding an equal amount of different concentrations of hypotonic saline solution into two cuvettes, one of which is incompletely dissolved The blood vessel, the hypotonic saline solution added to it cannot completely hemolyze the whole blood, and the other tube is completely hemolyzed, and the hypotonic saline solution added to it can completely hemolyze the whole blood; S2, put it into two cuvettes respectively Add an equal amount of whole blood, and after mixing, immediately detect the scattered light value A0 of the mixed solution in incomplete hemolysis, and the scattered light value B0 of the mixed solution in complete hemolysis; S3, at a specific time after the end of hemolysis, measure Scattered light value A1 of the mixed solution in incomplete hemolysis, B1 of the mixed solution in complete hemolysis; S4, according to the formula: percentage of hemolysis%=(A0‑A1) / (B0‑B1)×100% Percentage of blood vessel hemolysis. The detection result of the determination method is more accurate, and can realize standardization and consistency of results.
Description
technical field [0001] The invention relates to the technical field of measuring erythrocyte osmotic fragility, in particular to a method for measuring erythrocyte osmotic fragility and a kit thereof. Background technique [0002] The normal lifespan of mature red blood cells in the human body is about 120 days. During the survival period, they travel about 500 kilometers in the circulatory system and squeeze through blood vessels and pores that are much smaller than their own diameter many times. Therefore, red blood cells must have good toughness and deformability. Red blood cells mainly rely on the red blood cell membrane to maintain deformability and stability. If a certain protein on the membrane changes, it can cause hemolysis, such as hereditary spherocytosis. [0003] Erythrocyte osmotic fragility is a commonly used clinical index for testing red blood cell membrane defects. The erythrocyte osmotic fragility test is to measure the ability of red blood cells to withst...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More