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Erythrocyte osmotic brittleness measurement method and kit thereof

A technology of osmotic fragility and measurement method, which is applied in the field of erythrocyte osmotic fragility measurement method and its kit, which can solve the problems that the whole process of hemolysis cannot be reflected, the repeatability is difficult to guarantee, and the time control requirements are high.

Active Publication Date: 2019-12-03
深圳褀氏生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hemolysis rate at different time points is different. The rate method requires high time control, and the repeatability is difficult to guarantee.
In addition, the rate method cannot reflect the whole process of hemolysis

Method used

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  • Erythrocyte osmotic brittleness measurement method and kit thereof
  • Erythrocyte osmotic brittleness measurement method and kit thereof
  • Erythrocyte osmotic brittleness measurement method and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] This embodiment provides a method for measuring the osmotic fragility of red blood cells, and the specific steps include:

[0087] S0, equipped with incomplete hemolysis hypotonic saline solution and complete hemolysis hypotonic saline solution.

[0088] Incomplete hemolysis hypotonic saline solution contains:

[0089]4g / L sodium chloride

[0090] 0.1%(v / v) Proclin300

[0091] Completely hemolytic hypotonic saline solution contains:

[0092] 1g / L sodium chloride

[0093] 0.1%(v / v) Proclin300

[0094] S1. Add 1000uL of incomplete hemolytic hypotonic saline solution and complete hemolytic hypotonic saline solution into two cuvettes respectively;

[0095] S2. Add 6uL of whole blood to the above two cuvettes, mix well, start timing, and immediately use a scattering analyzer with a laser with a wavelength of 600nm as the light source to detect the scattering of the mixed solution in the incomplete hemolysis Light value A0, scattered light value B0 of the mixed solution...

Embodiment 2

[0099] This embodiment provides a kit suitable for the above-mentioned method for determining the osmotic fragility of red blood cells, including: a hypotonic saline solution with incomplete hemolysis and a hypotonic saline solution with complete hemolysis.

[0100] Incomplete hemolysis hypotonic saline solution contains:

[0101] 4g / L sodium chloride

[0102] 0.1%(v / v) Proclin300

[0103] Completely hemolytic hypotonic saline solution contains:

[0104] 1g / L sodium chloride

[0105] 0.1% (v / v) Proclin300.

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Abstract

The invention provides an erythrocyte osmotic brittleness measurement method and a kit thereof. The measurement method comprises the following steps: S1, respectively adding equal amounts of low-osmotic-pressure salt solutions with different concentrations into two cuvettes; to be specific, setting one cuvette to be an incomplete blood dissolving tube having the added low-osmotic-pressure salt solution not enabling whole blood to be hemolyzed and setting the othercuvette to be a complete blood dissolving tube having the added low-osmotic-pressure salt solution enabling whole blood to be hemolyzed; S2, respectively adding the same amount of whole blood into the two cuvettes, carrying out mixing uniformly, immediately detecting a scattered light value A0 of the mixed solution in the incompletely dissolved blood cuvette and the scattered light value B0 of the mixed solution in the completely dissolved blood cuvette; S3, measuring a scattered light value A1 of the mixed solution in the incompletely dissolved blood cuvette and a scattered light value B1 of the mixed solution in the completely dissolved blood cuvette at specific time after hemolysis finishing; and S4, calculating a hemolysis percentage of the incompletely dissolved blood cuvette according to a formula: hemolysis percentage%=(A0-A1) / (B0-B1)*100%. The detection result of the measurement method is more accurate, and result standardization and consistency can be realized.

Description

technical field [0001] The invention relates to the technical field of measuring erythrocyte osmotic fragility, in particular to a method for measuring erythrocyte osmotic fragility and a kit thereof. Background technique [0002] The normal lifespan of mature red blood cells in the human body is about 120 days. During the survival period, they travel about 500 kilometers in the circulatory system and squeeze through blood vessels and pores that are much smaller than their own diameter many times. Therefore, red blood cells must have good toughness and deformability. Red blood cells mainly rely on the red blood cell membrane to maintain deformability and stability. If a certain protein on the membrane changes, it can cause hemolysis, such as hereditary spherocytosis. [0003] Erythrocyte osmotic fragility is a commonly used clinical index for testing red blood cell membrane defects. The erythrocyte osmotic fragility test is to measure the ability of red blood cells to withst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/51
CPCG01N21/51G01N2021/513
Inventor 廉正鑫祁晖
Owner 深圳褀氏生物科技有限公司
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