Erythrocyte osmotic brittleness measurement method and kit thereof
A technology of osmotic fragility and measurement method, which is applied in the field of erythrocyte osmotic fragility measurement method and its kit, which can solve the problems that the whole process of hemolysis cannot be reflected, the repeatability is difficult to guarantee, and the time control requirements are high.
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Embodiment 1
[0086] This embodiment provides a method for measuring the osmotic fragility of red blood cells, and the specific steps include:
[0087] S0, equipped with incomplete hemolysis hypotonic saline solution and complete hemolysis hypotonic saline solution.
[0088] Incomplete hemolysis hypotonic saline solution contains:
[0090] 0.1%(v / v) Proclin300
[0091] Completely hemolytic hypotonic saline solution contains:
[0093] 0.1%(v / v) Proclin300
[0094] S1. Add 1000uL of incomplete hemolytic hypotonic saline solution and complete hemolytic hypotonic saline solution into two cuvettes respectively;
[0095] S2. Add 6uL of whole blood to the above two cuvettes, mix well, start timing, and immediately use a scattering analyzer with a laser with a wavelength of 600nm as the light source to detect the scattering of the mixed solution in the incomplete hemolysis Light value A0, scattered light value B0 of the mixed solution...
Embodiment 2
[0099] This embodiment provides a kit suitable for the above-mentioned method for determining the osmotic fragility of red blood cells, including: a hypotonic saline solution with incomplete hemolysis and a hypotonic saline solution with complete hemolysis.
[0100] Incomplete hemolysis hypotonic saline solution contains:
[0102] 0.1%(v / v) Proclin300
[0103] Completely hemolytic hypotonic saline solution contains:
[0104] 1g / L sodium chloride
[0105] 0.1% (v / v) Proclin300.
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