Method for detecting COX-2 enzymatic activity in process of evaluating analgesic effect of drug

Abstract

The invention relates to a method for detecting COX-2 enzymatic activity in a process of evaluating analgesic effect of a drug. The method is characterized by comprising the following steps: performing COX-2 enzymatic activity inhibition detection on the drug; and performing data processing and potency calculation on a COX-2 enzymatic activity detection result, so as to determine the analgesic effect of the drug. By utilizing a traditional Chinese medicine quality evaluation method provided by the invention and based on biological potency can perform quality evaluation on Chinese and Western analgesics or active components provided with corresponding targets, like a cassia twig tuckahoe preparation or an extract of cassia twig and tuckahoe, quality of the drug can be better controlled, andclinical medication effectiveness is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of quality control and evaluation of traditional Chinese medicines, in particular to a method for detecting COX-2 enzyme activity in the evaluation process of drug analgesia. Background technique [0002] The so-called pain in modern medicine is a complex physiological and psychological activity, and it is one of the most common clinical symptoms. It includes the pain sensation caused by noxious stimuli acting on the body, and the body's pain response to noxious stimuli (body motor response and / or visceral vegetative response, often accompanied by strong emotional color). Dysmenorrhea is a kind of pain. Among them, primary dysmenorrhea (primary dysmenorrhea) is functional dysmenorrhea, which refers to menstrual pain, often spasmodic, concentrated in the lower abdomen. Other symptoms include headache, fatigue, dizziness, nausea and vomiting, diarrhea, Lumbar and leg pain is very common in young women and aff...

AI Technical Summary

Problems solved by technology

For example, the high content of a single index compound does not mean that the overall drug quality is good, let alone that the overall drug is more effective

By controlling some single index compounds in compound Chinese medicine as an index of drug quality standards, although the amount of index components is accurately controlled, it is difficult to ensure the stability and uniformity of all components in traditional Chinese medicine, let alone Reflect the overall biological effect of traditional Chinese medicine and ensure the consistency of its clinical efficacy

[0005] However, there are few reports on the research results of biopotency assays in the field of Chinese herbal medicines.

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