Single nucleotide polymorphism (SNP) marker related to Chinese horseshort stature characters and application thereof

A Chinese national and dwarf technology, applied in the field of genetic biology, can solve the problems of lack of breeding markers, low level of breeding, low level of development and utilization, etc., and achieve the effect of accurate and reliable methods, easy operation, and promoting the breeding process

Active Publication Date: 2019-12-06
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low level of domestic pony breeding, the lack of breeding markers, and the low degree of development and utilization, the resource advantages of pony horses in my country cannot be transformed into economic advantages.
At present, foreign countries have located HMGA2 and other gene loci th...

Method used

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  • Single nucleotide polymorphism (SNP) marker related to Chinese horseshort stature characters and application thereof
  • Single nucleotide polymorphism (SNP) marker related to Chinese horseshort stature characters and application thereof
  • Single nucleotide polymorphism (SNP) marker related to Chinese horseshort stature characters and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Identification of polymorphic sites of TBX3 gene in Chinese national horse in embodiment 1

[0045] 1. Extract the genomic DNA from the blood of Chinese horses to be tested

[0046] Collected 210 ponies from Southwest China (including 19 from Debao, 34 from Baise, 35 from Wenshan, 20 from Yunnan, 15 from Lichuan, 32 from Jinjiang, 30 from Jianchang, 25 from Guizhou) and 308 normal-height horses (including 145 from Northwest China, 128 from Inner Mongolia, 24 from Northeast China, and 11 thoroughbreds), and 11 Przewalski's horses. A total of 529 individuals in 21 groups. Genomic DNA in blood was extracted by conventional methods.

[0047] Table 1 Sample Collection Form

[0048]

[0049]

[0050] 2. Amplify the nucleotide fragment containing the SNP site

[0051] Primers were designed according to the sequence of the TBX3 gene locus (gi 194246389:18100500-18101500 Equuscaballus isolate Twilight breed thoroughbred chromosome 8, EquCab2.0, wholegenome shotgun sequen...

Embodiment 2

[0059] Example 2 Correlation analysis and detection application of different genotypes and heights of Chinese national horses

[0060] The present invention has carried out a large number of studies on the correlation between genotype and height phenotype of Chinese national horses from different regions and breeds, and found that the SNP site provided by the present invention (located at the 501st position of the sequence shown in SEQ ID NO.1 , the polymorphism is G / A, corresponding to base pair 18205998 of horse chromosome 8) is significantly associated with domestic horse height (chi-square test P value<2.2e-16). The frequency of allele A in ponies was as high as 64.07%, while the frequency of allele G in common horses was 82.14%. The frequency of genotype AA was 65.95% in pony horses and 17.85% in common horses, and the average height of the horses with genotype AA was significantly shorter than that of horses with genotype GG (chi-square test P value<2.2e-16) , which is ...

Embodiment 3

[0062] Expanded population analysis of polymorphic sites in the TBX3 gene locus of 529 Chinese national horses. Using the chi-square test of R software to analyze, the above SNP site (located at the 501st position of the sequence shown in SEQ ID NO.1, the polymorphism is G / A, corresponding to the 18205998th base pair of horse No. 8 chromosome) The frequency of allele A in ponies was significantly higher than that in other average-height horses (non-ponies) (P<2.2e-16). The correlation between the allele A of the SNP locus and the dwarf traits of Chinese horses was further verified (as shown in Table 2).

[0063] Table 2 The number of genotypes of SNP loci in Chinese pony and common horse breeds

[0064]

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Abstract

The invention relates to a single nucleotide polymorphism (SNP) marker related to Chinese horse short stature characters. The SNP molecular marker is located at the 501th position of the sequence shown in SEQ ID NO.1, polymorphism is G/A, and the SNP molecular marker corresponds to the 18205998th base pair on the eighth chromosome of a horse. The single nucleotide polymorphism (SNP) marker relatedto the Chinese horse short stature characters and application thereof have the following advantages that (1) the molecular marker is not restricted by the age, sex and the like of Chinese horses, canbe used in early breeding of the Chinese horses, accurate screening can be even performed at birth, and the breeding process of the Chinese horse superior pony varieties can be significantly promoted; (2) a method for detecting mononucleotide polymorphism of a Chinese horse TBX3 gene is accurate and reliable, and operation is simple and convenient; and (3) detection of a SNP locus of the Chinesehorse TBX3 gene provides scientific basis for Chinese horse height size marker assisted selection and horse breeding.

Description

technical field [0001] The invention belongs to the field of genetic biology, and specifically relates to a SNP marker related to the dwarf trait of Chinese horses and its application. Background technique [0002] SNP single nucleotide polymorphism site mainly refers to the polymorphism of DNA fragments caused by the variation of a single deoxynucleotide on the genome deoxynucleic acid (DNA) sequence. The polymorphism of SNP only involves the variation of a single base, and the expression forms include substitution, insertion and deletion. [0003] SNP detection methods currently commonly used include Sanger sequencing, DNA chips, time-of-flight mass spectrometry, and the latest high-throughput next-generation sequencing technologies. As a genetic marker, SNP has been widely used in research fields such as gene mapping, cloning, genetic breeding and genetic diversity. [0004] Transcription factor TBX3 (T-Box transcription factor 3) is one of the key genes regulating grow...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124C12Q1/6876C12Q1/6837C12Q1/6895C12Q2600/13C12Q2600/16
Inventor 马月辉蒋琳刘雪雪浦亚斌张艳丽
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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