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A kind of small yellow croaker bone collagen antibacterial peptide and its application
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A technology of bone collagen and antimicrobial peptides, applied in application, specific peptides, connective tissue peptides, etc., to achieve the effects of enhanced antibacterial activity, strong synergy, and convenient operation
Active Publication Date: 2022-06-14
舟山市常青海洋食品有限公司
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Faced with such a huge bone resource, if it is not used or used irrationally, it will definitely bring about environmental pollution problems
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[0029] One embodiment of the present invention is a preparation method of small yellow croaker collagen antibacterial peptide, comprising the steps of:
[0030] Step 1: Trim, clean and break the small yellow croaker bones into 1-20mm fish bone particles;
[0031] Step 2: Add phosphate buffer (containing 0.01-0.05μM vanillin) with a pH of 8.0-9.0 to the fishbone particles according to the solid-liquid ratio of 1:1-2g / mL, grind with a tissue homogenizer, and Cook in a closed container at a temperature of 40-60°C for 15-30 minutes; in the process of aquatic product processing, the degree of heating of raw materials during high-temperature cooking will seriously affect the quality of the final product, but the temperature and time of cooking are not easy to control, which can easily lead to Insufficient denaturation of protein or excessive denaturation will affect the effect of enzymatic hydrolysis. And this step can change the collagen structure of small yellow croaker bone at a...
Embodiment 1
[0047] A preparation method of small yellow croaker collagen antibacterial peptide, comprising:
[0048] Step 1: Trim, clean and break the small yellow croaker bones into 1-20mm fish bone particles;
[0049] Step 2: According to the solid-liquid ratio of 1:1.2g / mL, add phosphate buffer solution (containing 0.02μM vanillin) with a pH of 8.5 to the fishbone particles, grind it with a tissue homogenizer, and grind it in a temperature of 50°C. Cook in a closed container for 20 minutes;
[0050] Step 2: Add alkaline protease and flavor protease to the octopus obtained in step 1, the enzyme-to-substrate ratio of alkaline protease is 500U / g, the enzyme-to-substrate ratio of flavor protease is 400U / g, and carry out enzymolysis at a temperature of 56°C 5h, and then inactivate the enzyme to obtain the enzymatic hydrolyzate;
[0051] Step 3: Add the enzymolysis solution obtained in step 2 into a centrifugal ultrafiltration tube with a molecular weight cut-off of 3kDa, centrifuge at a s...
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Abstract
The invention provides a small yellow croaker bone collagen antibacterial peptide and its application, which belong to the technical field of biology. The amino acid sequence of the antibacterial peptide is HECEDCYSGCRNSCQH, and the molecular weight is 1.87Da, which can inhibit the growth of food spoilage microorganisms on food and prevent or inhibit the growth of food spoilage microorganisms The spoilage of the food caused by microorganisms is actually non-toxic, has the advantages of low side effects or risks, and high safety; the antimicrobial peptide of the present invention can be used to prepare food preservatives to prevent or inhibit food spoilage caused by microorganisms; The combination of antibacterial peptide and coumarin of the present invention has strong synergistic effect, can enhance antibacterial activity, and reduce the usage amount of antibacterial peptide.
Description
technical field [0001] The invention belongs to the technical field of biology, and in particular relates to a small yellow croaker collagen antibacterial peptide and an application thereof. Background technique [0002] Collagen is a tissue structural protein in animals, accounting for about 25% of the total protein content of animals. Collagen is the most important fibrous protein in the extracellular matrix, the main fiber component of the body's connective tissue and interstitial tissue, and an important component of the cytoskeleton. Collagen is widely present in solid organs such as skin, tendons, bones, and ligaments, making tissues and organs have certain structural and mechanical properties, and can protect the body and support organs. Collagen polypeptide is the enzymatic hydrolysis product of collagen, its molecular weight is much lower than that of collagen, and its peptide chain has been broken, which can be directly absorbed and utilized by the human body. At...
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