One-pot synthesis method and application of anti-liver cancer drug selenite chitosan copper

A technology of chitosan copper and selenite, which is applied in the field of biomedical engineering, can solve the problems of high ratio, insufficient activity, and low selenization amount, so as to promote dissociation, eliminate side reactions, and reduce dissolution Effect

Active Publication Date: 2019-12-13
HUBEI UNIVERSITY OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is also further illustrated that chitosan directly reacts with selenous acid, and 2-amino and 6-hydroxyl participate in the reactivity not enough to achieve a high proportion of grafted selenous acid. Therefore, the amount of selenization is low

Method used

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  • One-pot synthesis method and application of anti-liver cancer drug selenite chitosan copper
  • One-pot synthesis method and application of anti-liver cancer drug selenite chitosan copper
  • One-pot synthesis method and application of anti-liver cancer drug selenite chitosan copper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 One-pot synthesis method of anti-liver cancer drug selenite esterified chitosan copper

[0042] 9.0g chitosan (molecular formula (C 6 h 11o 4 N·H 2 (O)n, equivalent to chitosan monomer 50mmol) is dissolved in 700mL mass fraction 1% formic acid solution, then add the aqueous solution of 12.5g copper sulfate pentahydrate (that is: 50mmol copper sulfate pentahydrate), stir at 60-70 ℃ After reacting for 1 h, adjust the pH value of the reaction solution to 7 with a 10% aqueous sodium hydroxide solution by mass fraction, stir the reaction for 1 h and wait for the pH value to decrease, then adjust the pH value of the reaction solution to 7 with a 10% aqueous sodium hydroxide solution again, Continue the reaction; repeat this for 2-3 times until the pH value no longer drops, and the complexation reaction is complete (the identification method is: take 2mL solution and add 2mL ethanol to precipitate the product, take the supernatant and add dropwise sodium hydrox...

Embodiment 2

[0057] The IR spectrum contrast of the selenite esterified chitosan copper prepared in embodiment 2 chitosan copper and embodiment 1

[0058] Compare chitosan copper [preparation by the method for preparing chitosan copper by literature (Wang Aiqin, etc., the synthesis and characterization of chitosan and Cu (Ⅱ) complexes [J], 2000 (03): 297-300)] and implementation The IR figure of the prepared selenite esterified chitosan copper pure product of example 1, the result is as follows figure 1 and figure 2 as shown, figure 2 It is easy to see in the IR diagram of selenized chitosan copper: compared with figure 1 In the IR spectrum of chitosan copper, the vibration peak of the Se=O double bond of very strong selenite is observed at 713cm-1, the free hydroxyl peak (3390cm-1) disappears, and the free hydroxyl is completely selenous After esterification, there is no influence of free hydroxyl groups, and the complexed hydroxyl groups and hydroxide groups in the compound can clea...

Embodiment 3

[0060] Anti-liver cancer activity test of embodiment 3 selenite esterified chitosan copper

[0061] Human hepatoma cells HepG2 were cultured in DMEM medium containing 10% fetal bovine serum and 100 U / mL of penicillin and streptomycin in a 37°C incubator with a CO2 concentration of 5% [15]. Change the medium every 1-2 days or when the medium turns light yellow. When the cells grew to 80% of the bottom area of ​​the flask, the cells were subcultured. Operate in an ultra-clean bench, first discard the old medium, wash gently with normal saline twice, add an appropriate amount of trypsin-EDTA to digest, observe the cells under the microscope to become round, quickly add new medium, and stop Digestion. Blow down the cells with a pipette gun, transfer to a centrifuge tube, centrifuge at 1 000r / min for 5min, discard the supernatant, resuspend in the new medium, count the cells with a hemocytometer, and pass passage at an appropriate ratio .

[0062] 96-well plate culture: Add Hep...

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Abstract

The invention discloses a one-pot synthesis method of an anti-liver cancer drug selenite chitosan copper. The one-pot synthesis method comprises the following steps: 1, dissolving chitosan in a formicacid solution to obtain a chitosan acid solution; 2, adding an aqueous copper sulfate solution into the chitosan acid solution, carrying out a complexation reaction under a heating condition for 1 h,and adjusting the pH value of the obtained solution to 7 for 2-3 times until the complexation reaction is complete and the pH value does not drop in order to obtain a product I; 3, heating the product I, adding an aqueous seleninic acid solution in batches, and carrying out a reaction to generate a product II; and 4, cooling the product II, adding isopyknic ethanol until no precipitate is generated, filtering the obtained solution, and washing and drying the precipitate to obtain the selenite chitosan copper. The invention further discloses an application of the selenite chitosan copper in the preparation of antitumor drugs. The method has the advantages of no great change of the pH value of the solution, avoiding of copper ion dissociation and other side reactions, ensuring of complete esterification of 6-hydroxyl groups of chitosan, and increase of the selenization amount.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, and in particular relates to a one-pot synthesis method and application of an anti-liver cancer drug selenite esterified chitosan copper. Background technique [0002] Chitosan [chitosan, referred to as CTS, molecular formula (C 6 h 11 o 4 N) n , also known as glucosamine, chitin sugar, scientific name β(1,4)-2-amino-2-deoxy-D-glucose], the molecular structure is as follows: [0003] [0004] It is an alkaline biological polysaccharide, which has various biological activities such as anti-tumor, hypolipidemic, anti-inflammatory, and antibacterial; it is positively charged under acidic conditions, and can be neutralized with anions on the surface of tumor cells through adsorption and discharge, targeting Good performance, non-toxic, no other adverse reactions, widely used in food additives. [0005] Selenium is an important component of glutathione peroxidase, which specific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61K31/722A61P35/00
CPCA61K31/722A61P35/00C08B37/003
Inventor 王红梅朱秀连曾小华陈琴华黄慧敏魏英吴最
Owner HUBEI UNIVERSITY OF MEDICINE
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