Fecal microorganism preserving fluid and preparation method thereof

Abstract

The invention discloses a fecal microorganism preserving fluid. The preserving fluid comprises a protein inhibitor, a stabilizer with a chelating agent and a preservative effect, a biological buffer solution, a metal chelating agent, sodium chloride and water, the final concentration of the protein inhibitor is 0.1-2 mol / L, the final concentration of the metal chelating agent is 1-100 mmol / L, thefinal concentration of the sodium chloride is 0.1-1 mol / L, the final concentration of the biological buffer solution is 1-100 mmol / L, and the final concentration of the stabilizer is 0.1% / L-10% / L. Theinvention further discloses a preparation method of the fecal microorganism preserving fluid, which comprises the follows several steps: step 1, adding 1 / 2 of sterile water by volume to a container;step 2, sequentially adding the sodium chloride, a Tris-CI buffer solution, ethylenediamine tetraacetic acid, the stabilizer and guanidine thiocyanate to the container; step 3, adding deionized waterto complete volume metering; and step 4, adjusting PH to 7.5, and performing sterilization and room-temperature cooling to obtain the preserving fluid. The preserving fluid has the advantages that thenormal-temperature preserving effect on samples is good, the preserving fluid is convenient to use, the whole preparation method is simple and the like.

Description

【Technical field】 [0001] The invention relates to the technical field of microbial sample detection and preservation, in particular to the technical field of a fecal microbial preservation solution and a preparation method thereof. 【Background technique】 [0002] After the intestinal microorganisms are isolated, if the nucleic acid cannot be extracted in time, the number, type and abundance of the flora will change. Feces are the source of intestinal microbial nucleic acid extraction. In order to improve the accuracy of intestinal gene detection, it is necessary to ensure the stability of intestinal flora after collecting feces. However, the feces tests in some existing hospitals or medical examination centers are limited by the location and experimental conditions. 1. Due to the limitation of testing personnel, timely detection and testing of stool samples cannot be carried out, and it is necessary to preserve the stool samples that cannot be tested in time. Traditional fe...

AI Technical Summary

Problems solved by technology

Feces are the source of intestinal microbial nucleic acid extraction. In order to improve the accuracy of intestinal gene detection, it is necessary to ensure the stability of intestinal flora after collecting feces. However, the feces tests in some existing hospitals or medical examination centers are limited by the location and experimental conditions. , Due to the limitation of testing personnel, timely detection and testing of stool samples cannot be carried out, and it is necessary to preserve the stool samples that cannot be tested in time

Traditional fecal samples are preserved by refrigeration, that is, the feces are collected and stored at a temperature of -20°C or lower, so that the intestinal microorganisms in them are at a low metabolic rate, and the number and composition of the flora are stabilized for a period of time. However, the refrigeration method is still limited by the sampling conditions, which makes the sample storage operation more complicated and the storage cost is higher, which is not conducive to the transportation of the sample, and the refrigeration process may also have a certain impact on the microbial activity and quality.

With the development of scientific research and technology, people use fecal microbial preservation solutions to preserve feces samples, but some existing fecal microbial preservation solutions have problems such as poor stability of fecal samples, short storage time, and complicated preparation methods.

Images