A method for analyzing sucrose esters in tobacco by multidimensional liquid chromatography-mass spectrometry

A multi-dimensional liquid chromatography and liquid chromatography mass spectrometry technology, which is applied in the field of chemical composition analysis in tobacco, can solve the problems of complex pretreatment and complex pretreatment steps, and achieve simple system construction, many adjustable parameters, and heavy detection workload Effect

Active Publication Date: 2022-07-08
SHANGHAI TOBACCO GRP CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

GC-MS method requires derivatization treatment, complicated pretreatment
The LC-MS method does not require derivatization, but still requires complex pretreatment steps, such as repeated liquid-liquid extraction, etc.

Method used

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  • A method for analyzing sucrose esters in tobacco by multidimensional liquid chromatography-mass spectrometry
  • A method for analyzing sucrose esters in tobacco by multidimensional liquid chromatography-mass spectrometry
  • A method for analyzing sucrose esters in tobacco by multidimensional liquid chromatography-mass spectrometry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0160] 1. Sample pretreatment

[0161] Take 0.2g of tobacco sample, add 0.1mL of internal standard sample and 5mL of acetonitrile for ultrasonic extraction for 20min, centrifuge for 10min under the condition of rotating speed 2000r / min, and obtain sample solution after passing through 0.22μm organic phase filter membrane. The internal standard sample was sucrose monocaprate.

[0162] 2. Determination

[0163] The sample solution was determined by two-dimensional liquid chromatography-mass spectrometry (LC-LC-MS). Perform quantification, and then determine the relative content of sucrose ester components in the sample solution that are similar in structure to the quantitatively determined internal standard sample.

[0164] Among them, the two-dimensional liquid chromatography-mass spectrometry (LC-LC-MS) method is used for the determination, and the target components are separated by the first-dimensional liquid chromatography. After the trapping column is eluted, the trappi...

Embodiment 2

[0179] The repeatability of the method was investigated by using oriental tobacco with high sucrose ester content, the pretreatment of step 1 in the above-mentioned embodiment 1 was carried out, and the LC-LC-MS analysis of step 2 in the above-mentioned embodiment 1 was carried out, and it was found that 6 types of different sucrose For ester and SOE, the repeatability of the method is about 10%, which meets the needs of detection. The results are shown in Table 6.

[0180] The method repeatability investigation of table 6 sucrose ester

[0181]

Embodiment 3

[0183] Select different types of tobacco samples, such as green tobacco leaf flue-cured tobacco, flue-cured tobacco, and oriental tobacco after flue-curing, carry out the determination of sucrose ester content, carry out the pretreatment of step 1 in above-mentioned embodiment 1, and carry out step 2 in above-mentioned embodiment 1 LC-LC-MS analysis, the specific determination results are shown in Table 7. It can be seen from Table 7 that compared with the flue-cured tobacco, the content of sucrose ester in oriental tobacco is generally higher, especially STE III, STE IV and STE V. It is possible that the contribution of such substances to the aroma components is greater. Compared with flue-cured tobacco and flue-cured tobacco, the content of STE II components was higher, and its relative content accounted for a higher proportion of total sucrose esters.

[0184] Table 7 Relative content of sucrose esters in actual samples

[0185]

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Abstract

The invention provides a method for analyzing sucrose esters in tobacco by multi-dimensional liquid chromatography-mass spectrometry. After adding an internal standard sample to a tobacco sample, a solvent is added for extraction, centrifugation and filtration to obtain a sample solution; Liquid chromatography-mass spectrometry was used for the determination. The sucrose ester components in the sample solution and the internal standard sample were qualitatively determined by mass spectrometry, and the internal standard sample in the sample solution was quantified by the standard curve method. The relative content of sucrose ester components with similar structure in the target sample. The present invention further provides a multi-dimensional liquid chromatography mass spectrometry analysis system and a using method thereof. The method for analyzing sucrose esters in tobacco by multi-dimensional liquid chromatography-mass spectrometry provided by the invention can effectively remove the interfering substances affecting the quantification of sucrose esters in tobacco, and has the characteristics of high accuracy, good reproducibility and low detection limit. And the instrument is completed automatically, which is suitable for batch testing of tobacco samples.

Description

technical field [0001] The invention belongs to the technical field of chemical composition analysis in tobacco, and relates to a method for analyzing sucrose esters in tobacco by multi-dimensional liquid chromatography-mass spectrometry, in particular to a method for analyzing sucrose esters in tobacco by using multi-dimensional liquid chromatography-mass spectrometry and heart cutting. method for the relative amounts of ingredients. Background technique [0002] Sucrose esters (SE) are an important class of aroma precursor substances in tobacco, which mainly exist in the waxy layer on the surface of tobacco leaves. There are 6 kinds of sucrose esters in tobacco, namely sucrose esters SE I, SE II, and SE III. , SE IV, SE V, and SEVI. For different types of tobacco leaves, the content of SE varies greatly. Among them, the content of SE in oriental tobacco was higher, and the content in burley and flue-cured tobacco samples was lower. SE itself has no special odor, but it ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88G01N30/74
CPCG01N30/06G01N30/88G01N30/74
Inventor 陈敏董惠忠刘百战吴达
Owner SHANGHAI TOBACCO GRP CO LTD
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