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Application of apocynum venetum leaf polyphenol

A high-sugar high-sugar technology, which is applied in the bioactive field of traditional Chinese medicine plant polyphenols, can solve the problems of stomach discomfort and patients' inability to take it for a long time, and achieve the effect of broad market and application

Active Publication Date: 2020-01-17
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Epalrestat, an aldose reductase inhibitor, is currently on the market, but sometimes it is accompanied by allergic symptoms such as erythema, blisters, and vomiting, and side effects such as stomach discomfort, and patients cannot take it for a long time
At present, there are many researches on the activity of polyphenols in Apocynum leaves, such as anti-oxidation, lowering blood pressure, and anti-depression. There is no report about the polyphenols in Apocynum leaves in diabetic retinopathy.

Method used

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  • Application of apocynum venetum leaf polyphenol
  • Application of apocynum venetum leaf polyphenol
  • Application of apocynum venetum leaf polyphenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] a. Take 25g of dried leaves of Apocynum venetum, heat it in a water bath with a concentration of 25 times the mass-volume ratio of 30% ethanol, and reflux it in a water bath at a temperature of 50°C. Extract 3 times for 2 hours. Collect the extract and recover the ethanol under reduced pressure;

[0026] b. Use a vacuum rotary evaporator at a temperature of 45°C to concentrate the extract from step a to 6.0 mg / mL of total polyphenols, and then purify the concentrated solution with HPD-450 macroporous resin. The adsorption rate of the sample solution is 1BV / h, then washed with water, the water elution volume is 3BV, the water washing flow rate is 2BV / h, and then eluted with 40% ethanol, the ethanol elution volume is 4BV, and the ethanol elution speed is 2BV / h;

[0027] c. Collect the ethanol eluate from step b and vacuum dry to obtain apocynum polyphenols.

Embodiment 2

[0029] a. Take 25g of dried leaves of Apocynum venetum, use 10 times the mass-volume ratio of 50% ethanol to heat and reflux in a water bath, the temperature of the water bath is 70℃, and extract 3 times for 2h. Collect the extract and recover the ethanol under reduced pressure;

[0030] b. Use a vacuum rotary evaporator at a temperature of 45°C to concentrate the extract in step a to 6.0 mg / mL of total polyphenols, and then purify the concentrated solution with HPD-300 macroporous resin. The adsorption rate of the loading solution is 1BV / h, then water washing, water elution volume is 4BV, water washing flow rate 3BV / h, and then eluted with 50% ethanol, ethanol elution volume is 4BV, ethanol elution speed is 2BV / h;

[0031] c. Collect the ethanol eluate from step b and vacuum dry to obtain apocynum polyphenols.

Embodiment 3

[0033] a. Take 25g of dried leaves of Apocynum venetum, use 30 times the mass-volume ratio of 70% ethanol to reflux in a water bath at a temperature of 70°C, extract 3 times for 2h, collect the extract, and recover the ethanol under reduced pressure;

[0034] b. Use a vacuum rotary evaporator at a temperature of 45°C to concentrate the extract from step a to 6.0 mg / mL of total polyphenols, and then purify the concentrated solution with HPD-600 macroporous resin. The adsorption rate of the loading solution is 1BV / h, then water washing, water elution volume is 5BV, water washing flow rate is 4BV / h, and then eluted with 70% ethanol, ethanol elution volume is 3BV, ethanol elution speed is 2BV / h;

[0035] c. Collect the ethanol eluate from step b and vacuum dry to obtain apocynum polyphenols.

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Abstract

The invention relates to application of apocynum venetum leaf polyphenol. The apocynum venetum leaf polyphenol is obtained from apocynum venetum leaves by extracting, purifying and refining. Accordingto in-vitro enzymatic activity screening and cell tests, the apocynum venetum leaf polyphenol has a function of treating retinopathy caused by high glucose damage, is capable of realizing in-vitro inhibition of aldose reductase activity, remarkably improving activity of Na<+>-K<+>-ATP enzymes in retinal pigment epitheliums, reducing the cell sorbitol content and regulating polyol pathways and hasthe function of treating retinopathy caused by high glucose damage. The apocynum venetum leaf polyphenol can be used for preparing various preparations with a function of improving retinopathy causedby high glucose damage.

Description

Technical field [0001] The invention belongs to the biological activity field of traditional Chinese medicine plant polyphenols, and relates to the use of apocynum venetum leaf polyphenols, in particular to the use of apocynum venetum leaf polyphenols in the preparation of medicines or health products for treating retinopathy caused by high-sugar injury. Cell experiments confirmed that it can improve retinopathy caused by high glucose damage. Background technique [0002] Apocynum (Apocynum venetum L.), apocynum venetum, belongs to the genus Apocynum. According to the records of "Chinese Flora", it is also called tea flower, wild hemp, Zeqima, daughter tea, tea seed, kenaf, Safflower, cattle tea, etc., are distributed in Xinjiang, Qinghai, Gansu, Shaanxi, Shanxi, Henan, Hebei, Jiangsu, Shandong, Liaoning, Inner Mongolia and other provinces in China. [0003] Apocynum venetum L., a natural plant in China, has a wide range of medicinal values ​​in the folk. The Compendium of Materia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/24A61P9/10
CPCA61K36/24A61P9/10
Inventor 信学雷彭军刘晓燕贺飞阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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