Method for determining content of p-hydroxyphenylacetic acid in a rhodiola rosea injection

A technology for the determination of p-hydroxyphenylacetic acid and its method, which is applied in the field of content determination of p-hydroxyphenylacetic acid in Dazhu Rhodiola Injection, and can solve the problems of discovering p-hydroxyphenylacetic acid and not yet discovering it

Active Publication Date: 2020-01-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the research on Dazhu Rhodiola Injection focuses on pharmacological effects and clinical applications, and there is no literature report on the discovery of p-hydroxyphenylacetic acid and its content determination in Dazhu Rhodiola Injection

Method used

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  • Method for determining content of p-hydroxyphenylacetic acid in a rhodiola rosea injection
  • Method for determining content of p-hydroxyphenylacetic acid in a rhodiola rosea injection
  • Method for determining content of p-hydroxyphenylacetic acid in a rhodiola rosea injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Chromatographic conditions: Chromatograph: Agilent 1200 HPLC; Chromatographic column: Agilent SB C-18 (4.6mm×250mm, 5μm); Mobile phase: Acetonitrile-0.05% phosphoric acid solution (5:95); Flow rate: 1.0mL min -1 ; Detection wavelength: 276nm; Column temperature: 30°C; Injection volume: 20 μL.

Embodiment 2

[0036] Determination of the content of p-hydroxyphenylacetic acid in Dazhu Rhodiola Injection by HPLC, the steps are as follows:

[0037] 1. Experimental equipment and chromatographic conditions

[0038] Chromatograph: Agilent 1200 high performance liquid chromatograph;

[0039] Detector: UV detector;

[0040] Chromatographic column: Unitary C-18 (4.6mm×250mm, 5μm);

[0041] Chromatographic conditions: mobile phase: acetonitrile (A)-0.05% phosphoric acid solution (B);

[0042] Gradient elution program: 0-200min, solution A: 5%-7%, solution B: 95%-93%;

[0043] Flow rate: 1.0mL·min -1 ;

[0044] Column temperature: 30°C;

[0045] Detection wavelength: 276nm;

[0046] Injection volume: 20 μL.

[0047] 2. Investigation of linear relationship

[0048] Accurately weigh 5.08 mg of p-hydroxyphenylacetic acid, dissolve it with an acetonitrile-water mixed solution with a volume ratio of 5:95 and set the volume to a 5 mL volumetric flask, shake well to obtain a standard stock s...

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Abstract

The invention discloses a method for determining content of p-hydroxyphenylacetic acid in a rhodiola rosea injection, and belongs to the field of traditional Chinese medicine chemical research. The rhodiola rosea injection is used as a raw material, silica gel column chromatography and preparative liquid phase separation and purification are carried out, C13-NMR and other spectroscopy means are used to prove the existence of p-hydroxyphenylacetic acid, and an HPLC determination method for determining the content of p-hydroxyphenylacetic acid is established on the basis.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine chemistry, in particular to a method for determining the content of p-hydroxyphenylacetic acid in Dazhu Rhodiola injection. Background technique [0002] Rhodiola wallichiana (HK.) S.H.Fu var.cholaensis (Praeger) S.H.Fu is a perennial herb of the Sedum family Rhodiola, also known as Rhodiola with thick stems. It was published in Shennong Materia Medica more than 2,000 years ago. It is recorded in "Jing Jing", "Qian Jin Yi Fang" in Tang Dynasty and "Compendium of Materia Medica" in Ming Dynasty, and it is mainly produced in Tibet, Sichuan and Yunnan. Modern research shows that the main chemical components of Rhodiola rosea include glycosides, coumarins, flavonoids, volatile oils, alkaloids, steroids, phenolic compounds and organic acids (gallic acid, oxalic acid, citric acid, malic acid, Succinic acid) and trace inorganic elements, etc., have obvious anti-tumor, anti-aging, anti-hypoxia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 李绪文任素娟金永日
Owner JILIN UNIV
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