Blood metabolite markers and their applications in the diagnosis of multiple myeloma

A technology for multiple myeloma and metabolites, applied in biological tests, instruments, measuring devices, etc., to achieve accurate and reliable results, good accuracy and high sensitivity

Active Publication Date: 2021-10-15
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No one has used plasma metabolite levels to diagnose MM

Method used

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  • Blood metabolite markers and their applications in the diagnosis of multiple myeloma
  • Blood metabolite markers and their applications in the diagnosis of multiple myeloma
  • Blood metabolite markers and their applications in the diagnosis of multiple myeloma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Screening Characterization of Differential Metabolites Between Healthy Humans and Multiple Myeloma Patients

[0028] 1. Objects and methods

[0029] 1. The source of the specimen

[0030] After obtaining the consent of the patients, bone marrow samples from 21 HD (healthy donors) and 72 newly diagnosed MM patients, peripheral blood samples from 29 healthy people and 71 newly diagnosed MM patients were collected. All samples were from Xiangya Hospital Affiliated to Central South University , The Third Xiangya Hospital Affiliated to Central South University, Tianjin Hematology Hospital, Chinese Academy of Medical Sciences. All MM patients were diagnosed as MM patients by detecting the type of serum monoclonal immunoglobulin and the proportion of bone marrow plasma cells. All MM patients were newly diagnosed and did not receive treatment to exclude treatment-induced metabolic changes. Both HD and MM patients were from the above three hospitals, and the age, s...

Embodiment 2

[0051] Example 2: Constructing a ROC curve to compare the ability of 5 differential metabolites to diagnose and distinguish MM patients from healthy people

[0052] SPSS23 software was used for ROC curve analysis, and AUC value was calculated, and the ability of 5 differential metabolites in the diagnosis of MM was judged by the abundance levels in bone marrow or peripheral blood. The AUC values ​​of 5 differential metabolites in bone marrow and peripheral blood were: bone marrow stearic acid: AUC=0.90, 95%CI: 0.83-0.95, P<0.0001, bone marrow palmitate: AUC=0.89, 95%CI: 0.81-0.95, P<0.0001, bone marrow aspartate: AUC=0.83, 95%CI: 0.73-0.90, P=0.0095, bone marrow proline: AUC=0.78, 95%CI: 0.65-0.88, P=0.0353 , bone marrow cystine: AUC=0.74, 95%CI: 0.62-0.83, P=0.0188, peripheral blood stearic acid: AUC=0.84, 95%CI: 0.75-0.91, P<0.0001, peripheral blood palmitic acid: AUC=0.82, 95%CI: 0.74-0.90, P<0.0001, peripheral blood aspartate: AUC=0.70, 95%CI: 0.60-0.79, P=0.0213, periphe...

Embodiment 3

[0057] Example 3: Application of 5 Metabolites in Diagnosis of MM

[0058] 1. Objects and methods

[0059] 1. The source of the specimen

[0060] After obtaining the consent of the patients, the peripheral venous blood plasma of 25 newly diagnosed patients with HD and 32 patients with MM in the Third Affiliated Hospital of Xiangya Hospital was collected. All MM patients were confirmed by the detection of serum monoclonal immunoglobulin and the proportion of bone marrow plasma cells. HD was age, sex, and body mass index matched to MM patients. The time of blood collection was in the morning on an empty stomach. Plasma samples were stored at -80°C.

[0061] 2. Main reagents

[0062]Acetonitrile, methanol, and formic acid (chromatographic grade) were purchased from Sigma-Aldrich; analytically pure chloroform and pyridine were purchased from China National Pharmaceutical Group Corporation; methoxyamine, N-methyl-N-(trimethylsilane) trifluoroethane Amide (containing 1% trimeth...

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Abstract

The invention relates to blood metabolite markers and applications for diagnosing multiple myeloma. First, through non-targeted metabolomics detection of bone marrow and peripheral blood samples from healthy donors (HD) and newly diagnosed patients with multiple myeloma (MM), five metabolites were screened out in bone marrow and peripheral blood. There are significant differences in blood, respectively L_Proline (proline), L_Aspartic acid (aspartic acid), L_Cystine (cystine), Stearic acid (stearic acid), Palmitic acid (palmitic acid). The results of ROC curve analysis showed that the five differential metabolites as a whole could diagnose MM more accurately. Targeted metabolomics detection further confirmed that the metabolite composed of 5 metabolites is an ideal method for MM diagnosis. The invention can accurately diagnose MM, and has great significance for promoting basic research, diagnosis and clinical treatment of MM.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to disease diagnosis markers, in particular to blood metabolite markers for diagnosing multiple myeloma, and the application of reagents for detecting blood metabolite markers in preparing preparations for diagnosing multiple myeloma. Background technique [0002] Multiple myeloma (Multiple myeloma, MM) is a malignant plasma cell disease that occurs in the bone marrow cavity. Osteolytic lesions, hypercalcemia, anemia, kidney damage and other clinical symptoms. MM is the second most common hematologic malignancy, accounting for about 15% of all hematologic malignancies in the United States, and 1% of all malignancies, and mostly occurs in the elderly over the age of 70. As China enters an aging society, the incidence of MM has increased in recent years. showing a clear upward trend. MM has no clinical symptoms in the early stage, so that it is mostly in the middle and late stage when it i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/68G01N30/02
CPCG01N30/02G01N33/57407G01N33/57488G01N33/6812G01N33/6815
Inventor 周文贾伟夏机良张靖宇伍璇武明花向娟娟
Owner CENT SOUTH UNIV
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