Microbial agent and its application to improve the cold and humidity resistance of pea
A technology of microbial agent and microbial strain, applied in the field of microbial agent for improving the cold and humidity resistance of pea, can solve the problems of pea cold and humidity resistance, irregular seedling emergence, low seedling rate, etc., and achieve environmental friendliness, Improved cold resistance and easy operation
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Embodiment 1
[0022] A microbial agent for improving the cold and humidity resistance of peas, the active ingredients of which are Lactobacillus plantarum YuN21 and Rhizobium, and the cfu ratio of Lactobacillus plantarum YuN21 and Rhizobium is 1:2.
[0023] The taxonomic name of Lactobacillus plantarum YuN21 Lactobacillus plantarum , has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, the preservation date is February 06, 2018, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.15346; Rhizobium commercially available Rhizobium.
[0024] The composition and content per liter of MRS liquid medium are: pea powder 30 g / L, lactose 15 g / L, diammonium hydrogen citrate 2 g / L, L-cysteine hydrochloride 0.05 g / L, blood red Vegetable 0.5 g / L, Calcium Carbonate 10 g / L, K 2 HPO 4 2 g / L, MgSO 4 ·7H 2 O 0.58 g / L, MnSO 4 ·H 2 O 0.25 g / L, Tween-80 1ml / L, pH 6.4, steri...
Embodiment 2
[0033] Activation of the strain: Take out the Lactobacillus plantarum YuN21 in the above-mentioned Example 1 stored at -20°C, use an inoculation loop to dip a little bacterial liquid on the MRS solid medium, and culture it at 37°C for 10-12 After 1 hour, pick a single colony on the MRS solid medium with an inoculation loop, inoculate it into a 100 mL Erlenmeyer flask containing 40 mL of MRS liquid medium, and culture it for 8 to 10 hours at a temperature of 37°C to obtain activated Lactobacillus ; At this point, each milliliter of MRS liquid medium contains at least 1 × 10 7 Lactobacillus cfu is a primary culture of Lactobacillus.
[0034] Preparation of microbial agent: mix the above-mentioned activated Lactobacillus plantarum YuN21 with the commercially available rhizobia at a cfu ratio of 1:2, and put the mixed bacterial liquid into the MRS liquid medium for fermentation according to the inoculation amount of 10%. Cultivation is carried out at a temperature of 30° C. for 18...
experiment example
[0037] The germination time of the pea seeds treated with microbial agents was 12-20 hours earlier. Three weeks later, the leaves of the seeds treated with the agents were small and thick. 2-4 mm longer than the roots of untreated control seeds;
[0038] In terms of coping with freezing damage and cold weather: January is the most serious stage of chilling damage stress for autumn sown peas. Compared with the pea plants in the control group that were not treated with bacterial agents, the pea plants treated with bacterial agents had short and thick stems, thick leaves, and fibrous roots. more, and the main root was 10-13 mm longer than that of the control group; after rapid emergence of the spring peas treated with the inoculum, with the increase of fibrous roots, the pea plants were robust, and compared with the control pea plants without inoculum treatment, the emergence was neat , The rate of rotten seeds caused by many days of cloudy and rainy weather is reduced by 45%, an...
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