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Method for degrading diethylstilbestrol by using Trametes versicolor crude enzyme solution

A technology of Coriolus versicolor and diethylstilbestrol, which is applied in the field of environmental restoration and can solve the problems of high cost of DES, secondary pollution, and difficult operation

Inactive Publication Date: 2020-02-28
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for degrading DES by using the crude enzyme solution of Coriolus versicolor to solve the technical problems of high cost, difficult operation and easy secondary pollution in the current DES degradation process

Method used

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  • Method for degrading diethylstilbestrol by using Trametes versicolor crude enzyme solution
  • Method for degrading diethylstilbestrol by using Trametes versicolor crude enzyme solution
  • Method for degrading diethylstilbestrol by using Trametes versicolor crude enzyme solution

Examples

Experimental program
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Effect test

Embodiment 1

[0039] (1) Preparation of Coriolus versicolor fermented liquid

[0040] Inoculate the strains of Trametes versicolor into the PDA comprehensive potato medium, activate and expand the culture at 27°C, and complete the activation when the strains cover the slope, and scrape an appropriate amount of activated mycelium to 80mL In the seed medium, vibrate culture in 30°C incubator for 4 days, inoculate the seed liquid after 4 days into the liquid fermentation medium according to the inoculation amount of 10%, and continue to vibrate culture in 30°C incubator for 3 days to obtain fermentation broth;

[0041] (2) Preparation of Coriolus versicolor crude enzyme liquid

[0042] Filter the fermented liquid obtained in step (1) with 8 layers of gauze to remove the spherical bacteria, and obtain the filtered liquid, which is centrifuged at 4°C and 10,000 r / min for 15 minutes, and the centrifuged supernatant is the color Coriolus versicolor extracellular crude enzyme liquid, the enzyme a...

Embodiment 2

[0049] (1) Preparation of Coriolus versicolor fermented liquid

[0050] Inoculate the strains of Trametes versicolor into the PDA comprehensive potato medium, activate and expand the culture at 27°C, and complete the activation when the strains cover the slope, and scrape an appropriate amount of activated mycelium to 80mL In the seed medium, vibrate culture in 30°C incubator for 4 days, inoculate the seed liquid after 4 days into the liquid fermentation medium according to the inoculation amount of 10%, and continue to vibrate culture in 30°C incubator for 3 days to obtain fermentation broth;

[0051] (2) Preparation of Coriolus versicolor crude enzyme liquid

[0052] Filter the fermented liquid obtained in step (1) with 8 layers of gauze to remove the spherical bacteria, and obtain the filtered liquid, which is centrifuged at 4°C and 10,000 r / min for 15 minutes, and the centrifuged supernatant is the color Coriolus versicolor extracellular crude enzyme liquid, the enzyme a...

Embodiment 3

[0056] (1) Preparation of Coriolus versicolor fermented liquid

[0057] Inoculate the strains of Trametes versicolor into the PDA comprehensive potato medium, activate and expand the culture at 27°C, and complete the activation when the strains cover the slope, and scrape an appropriate amount of activated mycelium to 80mL In the seed medium, vibrate culture in 30°C incubator for 4 days, inoculate the seed liquid after 4 days into the liquid fermentation medium according to the inoculation amount of 10%, and continue to vibrate culture in 30°C incubator for 3 days to obtain fermentation broth;

[0058] (2) Preparation of Coriolus versicolor crude enzyme liquid

[0059] Filter the fermented liquid obtained in step (1) with 8 layers of gauze to remove the spherical bacteria, and obtain the filtered liquid, which is centrifuged at 4°C and 10,000 r / min for 15 minutes, and the centrifuged supernatant is the color Coriolus versicolor extracellular crude enzyme liquid, the enzyme a...

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Abstract

The invention discloses a method for degrading diethylstilbestrol by using a Trametes versicolor crude enzyme solution, and belongs to the technical field of environmental remediation, wherein the method comprises three steps of preparation of a Trametes versicolor fermentation broth, preparation of a Trametes versicolor crude enzyme liquid, and utilizing. In a process for degrading diethylstilbestrol by using a Trametes versicolor crude enzyme solution, 100-150 [mu]L of a 2.5 g / L DES-methanol solution and 1-2 mL of a Trametes versicolor extracellular crude enzyme solution are added, a 7.85-8.9 mL of an acetic acid buffer solution is added, and a reaction is performed for 3-4 h to complete the degradation of diethylstilbestrol. According to the invention, the method adopts a biological enzyme catalysis process, and has characteristics of high diethylstilbestrol removal rate, safety, no pollution and the like.

Description

technical field [0001] The invention relates to the technical field of environmental restoration, in particular to a method for degrading diethylstilbestrol by using the crude enzyme solution of Coriolus versicolor. Background technique [0002] Diethylstilbestrol (DES), as an environmental estrogen, is a lipophilic substance with relatively stable properties, not easy to be degraded, easy to remain in the fat of human and animal tissues, and long-term use can cause liver damage. A large number of studies have shown that long-term intake of diethylstilbestrol by aquatic organisms will cause sex conversion, resulting in difficulty in the reproduction of aquatic organisms, and ultimately accelerated the extinction of species. During the production process, diethylstilbestrol will be discharged into the environment along with the production wastewater, and enriched in water and soil, which poses a serious threat to the environment and human health that humans depend on for surv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/14C12R1/645
CPCC02F3/342C12N1/14C02F2101/345
Inventor 刘红艳谢世伟唐琳张文强李培骏单杨
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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