Chinese pumpkin mutant induction method

Abstract

The invention provides a Chinese pumpkin mutant induction method, including the steps of (1), soaking plump and uniform pumpkin seeds in water and carrying out germination accelerating treatment, andthen soaking the seeds in an ethylmethane sulfonate solution with volume concentration being 1.6-1.8% for 12 hours; (2), cleaning the seeds treated in the step (1) with clean water, spreading the cleaned seeds in culture vessels of which the internals are covered with wet filter paper, accelerating germination in an incubator, and sowing seeds, growing seedlings and planting in field sequentially;(3), pinching when plants grow 5 leaves and 1 bud tip, pruning and remaining two vines each plant, and marking the plants as the M1 generation; (4), observing characters of plants in the M1 generation, and performing same-vine selfing to reserve seeds to obtain selfined pumpkin seeds, marking the seeds as the M2 generation; observing characters of plants in the M2 generation, and screening to obtain the mutants. By the Chinese pumpkin mutant induction method for induction of pumpkin, the seed germination rate is 59%, the total phenotypic mutation frequency of the plants in the M2 generation is up to 11.2%, and thus, a Chinese pumpkin mutant library can be constructed effectively.

Description

technical field

[0001] The invention belongs to the field of agricultural technology, and in particular relates to a mutagenesis method for Chinese pumpkin mutants. Background technique

[0002] Chinese squash (Cucurbita moschata D.) is one of the four cultivars of the genus Cucurbita, and is an important vegetable crop of Cucurbitaceae, which has a wide range of nutritional, medicinal and economic values. The related research of pumpkin in my country is mainly on variety selection and breeding, the basic research is weak, and the genetic laws of many agronomic traits have not been fully grasped so far, which seriously restricts the development of conventional breeding. The rapid development of genomics has brought hope to break the limitations of conventional breeding, and the discovery and functional research of target trait genes have become the focus of genomics research. Forward and reverse genetics methods to analyze the genome are two effective ways of functional gen...

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