Preparation method of hericium erinaceus polysaccharide protein extract
A protein extract and Hericium erinaceus polysaccharide technology, applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of long extraction time, destruction of polysaccharide structure, high cost, etc., and achieve good uniformity and consistency. The extraction process is simple and the effect of avoiding friction
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0026] Weigh 100g of the fruiting body of Hericium erinaceus, grind the powder through an 80 mesh sieve to obtain Hericium erinaceus powder, and ultrasonically break the wall of the obtained Hericium erinaceus powder, the ultrasonic frequency is 15KHz, the ultrasonic time is 5min, add 200ml of 1mol / L NaCl solution to dissolve And extract at 30°C, move into a high-pressure tank, fill the high-pressure tank with nitrogen, make the pressure of the high-pressure tank 5500kPa, dissolve a large amount of nitrogen in the cell suspension, open the pressure reducing valve, and reduce the pressure in the tank to within 1-3min. Under normal atmospheric pressure, take out the Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, concentrate it to 1 / 8 of the original body by rotary evaporation, add 5 times the volume of absolute ethanol to precipitate at 4°C for 16h, centrifuge at 5000r / min for 20min, and collect Precipitate and dry at 50°C to obtain Hericium e...
Embodiment 2
[0029] Take Hericium erinaceus fruiting body 100g, grind powder and cross 80 mesh sieves, obtain Hericium erinaceus powder, ultrasonically break the wall of gained Hericium erinaceus powder, ultrasonic frequency is 20KHz, ultrasonic time 10min, add 200ml 0.5mol / LNaCl solution to dissolve and Extract at 40°C, move into a high-pressure tank, fill the high-pressure tank with carbon dioxide, make the pressure of the high-pressure tank 4000kPa, dissolve a large amount of carbon dioxide in the cell suspension, open the pressure reducing valve, and reduce the pressure in the tank to normal within 1-3min Atmospheric pressure, take out Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, concentrate by rotary evaporation to 1 / 8 of the original body, add 5 times the volume of absolute ethanol to precipitate at 4°C for 16h, centrifuge at 5000r / min for 20min, and collect the precipitate , and dried at 50°C to obtain Hericium erinaceus polysaccharide-protein c...
Embodiment 3
[0032] Take Hericium erinaceus fruiting body 100g, grind powder and cross 80 mesh sieves, obtain Hericium erinaceus powder, ultrasonically break the wall of gained Hericium erinaceus powder, ultrasonic frequency is 20KHz, ultrasonic time 8min, add 200ml 0.8mol / LNaCl solution to dissolve and Extract at 45°C, move into a high-pressure tank, fill the high-pressure tank with carbon dioxide, make the pressure of the high-pressure tank 4500kPa, dissolve a large amount of carbon dioxide in the cell suspension, open the pressure reducing valve, and reduce the pressure in the tank to normal within 1-3min Atmospheric pressure, take out Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, concentrate by rotary evaporation to 1 / 8 of the original body, add 5 times the volume of absolute ethanol to precipitate at 4°C for 16h, centrifuge at 5000r / min for 20min, and collect the precipitate , and dried at 50°C to obtain Hericium erinaceus polysaccharide-protein co...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com