Preparation method of hericium erinaceus polysaccharide protein extract

A protein extract and Hericium erinaceus polysaccharide technology, applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of long extraction time, destruction of polysaccharide structure, high cost, etc., and achieve good uniformity and consistency. The extraction process is simple and the effect of avoiding friction

Active Publication Date: 2020-03-24
福州福德恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there are multiple methods for extracting Hericium erinaceus polysaccharides, such as utilizing heat treatment to destroy the cell structure, promoting the release and transfer of intracellular substances, thereby extracting polysaccharides, but the extraction time is long and the extraction rate is low; while acid extraction, Alkaline extraction is a method of extracting polysaccharides by chemically destroying the cell structure. While destroying the cell wall and other structures, it will also destroy the structure of polysaccharides, so it is not commonly used; with the promotion of enzyme preparations, enzymatic hydrolysis is gradually applied to the extraction of polysaccharides , mainly using the enzymatic hydrolysis of pectinase to achieve the purpose of destroying the cell structure, but it is limited due to its high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 100g of the fruiting body of Hericium erinaceus, grind the powder through an 80 mesh sieve to obtain Hericium erinaceus powder, and ultrasonically break the wall of the obtained Hericium erinaceus powder, the ultrasonic frequency is 15KHz, the ultrasonic time is 5min, add 200ml of 1mol / L NaCl solution to dissolve And extract at 30°C, move into a high-pressure tank, fill the high-pressure tank with nitrogen, make the pressure of the high-pressure tank 5500kPa, dissolve a large amount of nitrogen in the cell suspension, open the pressure reducing valve, and reduce the pressure in the tank to within 1-3min. Under normal atmospheric pressure, take out the Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, concentrate it to 1 / 8 of the original body by rotary evaporation, add 5 times the volume of absolute ethanol to precipitate at 4°C for 16h, centrifuge at 5000r / min for 20min, and collect Precipitate and dry at 50°C to obtain Hericium e...

Embodiment 2

[0029] Take Hericium erinaceus fruiting body 100g, grind powder and cross 80 mesh sieves, obtain Hericium erinaceus powder, ultrasonically break the wall of gained Hericium erinaceus powder, ultrasonic frequency is 20KHz, ultrasonic time 10min, add 200ml 0.5mol / LNaCl solution to dissolve and Extract at 40°C, move into a high-pressure tank, fill the high-pressure tank with carbon dioxide, make the pressure of the high-pressure tank 4000kPa, dissolve a large amount of carbon dioxide in the cell suspension, open the pressure reducing valve, and reduce the pressure in the tank to normal within 1-3min Atmospheric pressure, take out Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, concentrate by rotary evaporation to 1 / 8 of the original body, add 5 times the volume of absolute ethanol to precipitate at 4°C for 16h, centrifuge at 5000r / min for 20min, and collect the precipitate , and dried at 50°C to obtain Hericium erinaceus polysaccharide-protein c...

Embodiment 3

[0032] Take Hericium erinaceus fruiting body 100g, grind powder and cross 80 mesh sieves, obtain Hericium erinaceus powder, ultrasonically break the wall of gained Hericium erinaceus powder, ultrasonic frequency is 20KHz, ultrasonic time 8min, add 200ml 0.8mol / LNaCl solution to dissolve and Extract at 45°C, move into a high-pressure tank, fill the high-pressure tank with carbon dioxide, make the pressure of the high-pressure tank 4500kPa, dissolve a large amount of carbon dioxide in the cell suspension, open the pressure reducing valve, and reduce the pressure in the tank to normal within 1-3min Atmospheric pressure, take out Hericium erinaceus cell suspension, centrifuge at 5000r / min to get the supernatant, concentrate by rotary evaporation to 1 / 8 of the original body, add 5 times the volume of absolute ethanol to precipitate at 4°C for 16h, centrifuge at 5000r / min for 20min, and collect the precipitate , and dried at 50°C to obtain Hericium erinaceus polysaccharide-protein co...

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Abstract

The invention discloses a preparation method of a hericium erinaceus polysaccharide protein extract. The preparation method comprises the following steps: S1, obtaining hericium erinaceus powder; s2,performing ultrasonic wall breaking, and soaking in an extracting solution; s3, introducing an inert gas until the pressure is 4000-5500 kpa, and rapidly recovering to normal atmospheric pressure to obtain hericium erinaceus cell suspension; and s4, conducting centrifugal separation, and precipitating supernate through ethyl alcohol to obtain the hericium erinaceus polysaccharide protein extract;the hericium erinaceus polysaccharide protein is extracted evenly and thoroughly, the extraction speed is high, operation is easy and convenient, the structural integrity of the polysaccharide proteinis protected, the extraction rate is high, pure physical extraction is achieved, and the hericium erinaceus polysaccharide protein extract is non-toxic, harmless and low in cost.

Description

technical field [0001] The invention relates to the field of Hericium erinaceus, in particular to a preparation method of a polysaccharide protein extract of Hericium erinaceus. Background technique [0002] Hericium erinaceus polysaccharide protein has good health care functions, such as treating gastritis and gastric ulcer, lowering blood sugar, anti-radiation and improving immunity, scavenging free radicals, anti-aging, protecting liver and liver, strengthening stomach and spleen, etc. Function, can induce tumor cell apoptosis, strongly inhibit the activity of tumor cell telomerase, can promote the generation of hematopoietic stem cells and progenitor cells in mice, and improve their hematopoietic function. It is a good antioxidant and has certain antioxidant properties. Features. [0003] Therefore, it is of great significance to obtain complete and high-purity Hericium erinaceus polysaccharide protein. [0004] In the prior art, there are multiple methods for extracti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07K1/32C07K1/14
CPCC07K1/145C07K1/32C08B37/0003
Inventor 叶艳鹏方柳慧郑秋芳方成
Owner 福州福德恩生物科技有限公司
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