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Method for determining concentration of ibuprofen in blood plasma by liquid chromatography-mass spectrometry

A LC/MS and plasma technology, applied in the field of medicine, can solve the problems of speed, precision, sensitivity, selectivity, etc. to be improved, and achieve the effects of simple pretreatment method, good peak shape and stable baseline.

Inactive Publication Date: 2020-03-27
徐州立兴佳正医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the speed, precision, sensitivity and selectivity of the existing determination methods for ibuprofen need to be improved

Method used

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  • Method for determining concentration of ibuprofen in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of ibuprofen in blood plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of ibuprofen in blood plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] Example: Human K 2 Determination of Ibuprofen Concentration in EDTA Plasma

[0034] 1. Experimental materials and analytical equipment

[0035] Ibuprofen (analyte): USP or equivalent or higher grade standard

[0036] Ibuprofen-d3 (internal standard): TLC Pharmaceutical Standards or equivalent or higher grade standard

[0037] The reagents used are shown in Table 1 below:

[0038] Table 1 Reagent Details

[0039] Reagent name level manufacturer Acetonitrile HPLC J. T. Baker Methanol HPLC J. T. Baker Ammonium acetate HPLC J. T. Baker

[0040] Note: Reagents of the same grade or higher can also be used

[0041] The analytical equipment used is shown in Table 2 below:

[0042] Table 2 Details of equipment used

[0043]

[0044]

[0045] The same LC / MS / MS system can also be used.

[0046] 2. Liquid conditions

[0047] 1. Liquid chromatography conditions

[0048] Chromatographic column: Agilent ZORBAX XDB-C18, 5μm, ...

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Abstract

The invention discloses a method for determining the concentration of ibuprofen in blood plasma by liquid chromatography-mass spectrometry, which adopts a liquid chromatography-mass spectrometry system for determination, and comprises the following steps: taking a sample to be determined, adding a certain amount of mixed organic solvent for extraction and pretreatment, performing separating by using a chromatographic column, and performing detecting by a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of ibuprofen; according to the method, the linear range of the plasma standard curve is 0.1-40 mu g / mL, the intra-batch precision RSD andthe inter-batch precision RSD are both smaller than + / -15%, and the method is suitable for measuring the concentration of ibuprofen in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for measuring medicines, in particular to a method for measuring the concentration of ibuprofen in blood plasma by liquid chromatography-mass spectrometry. Background technique [0002] Ibuprofen is the only antipyretic drug for children jointly recommended by the World Health Organization and the US FDA. It is recognized as the first choice of non-steroidal anti-inflammatory drugs for children. It has anti-inflammatory, analgesic and antipyretic effects. Its chemical name is 2-methyl-4-(2-methylpropyl)phenylacetic acid, molecular formula: C 13 h 18 o 2 , molecular weight: 206.29, its chemical structural formula is: [0003] [0004] Non-steroidal anti-inflammatory drugs (NSAIDs) are a class of anti-inflammatory drugs that do not contain a steroidal structure. This drug exerts its antipyretic, analgesic, and anti-inflammatory effects by inhibiting the synthesis o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/34G01N30/72
CPCG01N30/06G01N30/34G01N30/72G01N30/88G01N2030/045G01N2030/062G01N2030/8822G01N2030/884
Inventor 林明弘
Owner 徐州立兴佳正医药科技有限公司
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