Anti-saccharification mask containing mesenchymal stem cell culture supernatant and preparation method thereof

A technology for the preparation of stem cells and facial masks, which is applied in the field of anti-glycation facial masks and its preparation, can solve the problems of anti-glycation facial masks, and achieve the effects of improving skin barrier function, reducing fine lines, improving wrinkles and skin texture

Inactive Publication Date: 2020-03-31
盛世荣恩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The inventor found that the anti-glycation products provided by the prior art have not yet had an efficient anti-glycation mask containing mesenchymal stem cell culture supernatant

Method used

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  • Anti-saccharification mask containing mesenchymal stem cell culture supernatant and preparation method thereof
  • Anti-saccharification mask containing mesenchymal stem cell culture supernatant and preparation method thereof
  • Anti-saccharification mask containing mesenchymal stem cell culture supernatant and preparation method thereof

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Embodiment 1

[0035] see Figure 1-6 , the present invention provides a technical solution: a method for preparing an anti-glycation mask containing mesenchymal stem cell culture supernatant, comprising the following steps:

[0036] (1) Preparation of culture supernatant of mesenchymal stem cells: preparation of culture supernatant of mesenchymal stem cells: pretreatment of cell culture containers with cohesin, cell culture containers can be cell culture dishes, cell culture bottles and other containers After pretreatment, the cohesin was sucked away, and then the acclimatized P1 generation mesenchymal stem cells were plated in the culture container and the medium was changed on the second day, and the culture supernatant was collected on the third day, and passed through a 0.22 μm filter membrane for later use ;

[0037] (2) Preparation of base liquid: 8% glycerol, 2% butanediol, 1% 1,2-pentanediol, 0.2% dipropylene glycol, 0.5% diglycerol, 1% sucrose, 0.2% sodium PCA, 0.2% PEG-60 hydrog...

Embodiment 2

[0052] A method for preparing an anti-glycation mask containing mesenchymal stem cell culture supernatant, comprising the steps of:

[0053] (1) Preparation of mesenchymal stem cell culture supernatant: pretreat the cell culture container with cohesin, suck off the cohesion after pretreatment, and then plate the acclimated P15 generation mesenchymal stem cells in the culture container And change the medium on the second day, collect the culture supernatant on the third day, and pass it through a 0.22 μm filter membrane for later use;

[0054] (2) Preparation of base liquid: 10% glycerin, 2% butanediol, 1% 1,2-pentanediol, 0.2% dipropylene glycol, 0.5% diglycerol, 1% sucrose, 0.5% sodium PCA, 0.2% PEG-60 hydrogenated castor oil, 0.2% xanthan gum and the rest of water A component is heated to 80°C in a high-speed mixer and mixed at a high speed. When the high-speed mixer is cooled to 35°C, put in the The B component of 0.1% citric acid, 0.1% sodium citrate and the balance of wa...

Embodiment 3

[0069] A method for preparing an anti-glycation mask containing mesenchymal stem cell culture supernatant, comprising the steps of:

[0070] (1) Preparation of mesenchymal stem cell culture supernatant: pretreat the cell culture container with cohesin, suck away the cohesion after pretreatment, and then plate the acclimated P30 mesenchymal stem cells in the culture container And change the medium on the second day, collect the culture supernatant on the third day, and pass it through a 0.22 μm filter membrane for later use;

[0071] (2) Preparation of base liquid: 5% glycerin, 4% butanediol, 5% 1,2-pentanediol, 5% dipropylene glycol, 5% diglycerin, 4% sucrose, 3% sodium PCA, 1% PEG-60 hydrogenated castor oil, 0.3% xanthan gum and the rest of water A component is heated to 80°C in a high-speed mixer and mixed at a high speed. When the high-speed mixer is cooled to 35°C, put in the The component B of 0.5% citric acid, 0.5% sodium citrate and the balance of water in mass ratio i...

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Abstract

The invention discloses a preparation method of an anti-saccharification mask containing a mesenchymal stem cell culture supernatant. The method comprises the following steps: preparing the mesenchymal stem cell culture supernatant, preparing substrate liquid, cooling a high-speed mixer to room temperature, adding the above mesenchymal stem cell culture supernatant obtained in the above substrateliquid, and carrying out high-speed mixing and stirring to obtain the anti-saccharification mask containing the mesenchymal stem cell culture supernatant, and wherein a mask solution is prepared fromthe following components in percentage by mass: 0.1 to 5 percent of the mesenchymal stem cell culture supernatant and 95 to 99.9 percent of the substrate liquid. According to the invention, the mesenchymal stem cell culture supernatant is combined with an anti-saccharification functional component; compared with anti-saccharification functional components such as the supernatant or resveratrol, carnosine, lipoic acid and the like cultured only by using mesenchymal stem cells, the composition has a better skin improvement effect, increases skin elasticity, brightens skin color and glossiness, improves skin barrier function, locks water, moisturizes, improves wrinkles, skin texture and the like, and solves skin problems from an internal cell level.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to an anti-glycation mask containing mesenchymal stem cell culture supernatant and a preparation method thereof. Background technique [0002] With the development of society and the improvement of people's living standards, people pay more attention to their own skin care and skin care, especially women. However, there are many kinds of cosmetics on the market with large differences in efficacy, and it is difficult for consumers to distinguish them. In order to pursue the whitening effect, some fine chemical skin care products add substances harmful to human skin such as lead and mercury, which cause skin inflammation and infection. On the other hand, the current cosmetic products contain few biologically active substances, which cannot fundamentally improve the growth status and metabolism of epidermal cells, and have limited repairing effect on the skin. [0003] As people gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/34A61K8/44A61K8/49A61K8/64A61K8/73A61K8/9728A61K8/9789A61K8/98A61Q19/00A61Q19/08
CPCA61K8/99A61K8/9728A61K8/34A61K8/44A61K8/731A61K8/64A61K8/4986A61K8/347A61K8/9789A61K8/987A61K8/345A61Q19/08A61Q19/00A61K2800/805
Inventor 李慧芳王悦朋孙爽
Owner 盛世荣恩生物科技有限公司
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