Far-field super-resolution reconstruction method based on Fourier laminated imaging
A technology of super-resolution reconstruction and Fourier stacking, applied in image data processing, graphic image conversion, instruments, etc., can solve the problem of no far-field application, and achieve the effect of reducing reconstruction time and algorithm complexity.
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[0062] The present invention will be described in detail below in conjunction with the accompanying drawings.
[0063] Step 1: Build and debug hardware system
[0064] figure 1 It is a schematic diagram of the built system, in which the plane of the sample, the scattering sheet and the plane of the objective lens are parallel to each other. The sample is located in the far field of the objective lens, and the laser light is perpendicular to the sample plane.
[0065] When debugging the system, first remove the sample, control the drive displacement device to move in the Z direction, make the scattering device located at the focal plane, and then fix the sample for image acquisition.
[0066] Step 2: image acquisition;
[0067] During image acquisition, all other light sources except the laser need to be turned off. The computer drives the displacement device to move in the shape of "back" in the X-Y plane, and the step size of each movement is 1 mm. One image acquisition ...
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