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Bacterium inhibiting feed and preparation method and application thereof

A feed, yeast technology, applied in the application, animal feed, animal feed and other directions, can solve the problem of lack of animal replacement test verification

Pending Publication Date: 2020-04-07
安琪酵母(柳州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing yeast-related antibiotic substitute products involve the field of microbial engineering bacteria. Some yeast species, such as Torula delbei, do not exist in the feed additive catalog published by the Ministry of Agriculture, and genetically modified bacteria such as Saccharomyces cerevisiae are still listed with At the research stage of in vitro antibacterial test, there is a lack of verification of relevant animal in vivo antibacterial test

Method used

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  • Bacterium inhibiting feed and preparation method and application thereof
  • Bacterium inhibiting feed and preparation method and application thereof
  • Bacterium inhibiting feed and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] The preparation of embodiment one yeast essence

Embodiment 11

[0092] (1) Preparation of yeast essence

[0093] The yeast essence is prepared by the following method:

[0094] (1) Adopt Saccharomyces cerevisiae strain Saccharomyces cerevisiae Z2.2 (Saccharomyces cerevisiae HansenZ2.2), the preservation number is CCTCC NO: M205128, the source and preparation method of the strain are recorded in the Chinese patent application number 200710135740.6, publication number CN101361524A Patent pending. Observed by an optical microscope, the cell diameter of the Saccharomyces cerevisiae strain is about 4-6 μm, which is ellipsoidal and reproduces asexually by budding; after being cultured on a solid medium plate at 28°C for 24 hours, it becomes a milky white, opaque, round colony.

[0095] Inoculate the slant of the strain into 100mL shake flask liquid culture medium with 2 rings of inoculum, place the shaker for cultivation, set the rotation speed at 250r / min, and cultivate at 30°C for 18h. Wherein the formula of the 100mL liquid shake flask cult...

Embodiment 12

[0211] The yeast essence is prepared by the following method, and the content of the prepared yeast essence is determined, and its antibacterial effect and solubility are determined.

[0212] (1) Preparation of yeast essence

[0213] The yeast essence is prepared by the following method:

[0214] (1) Saccharomyces cerevisiae FX-2 (Saccharomyces cerevisiae FX-2), a species of Saccharomyces cerevisiae, is used, and the deposit number is CCTCC NO: M2016418. The strain comes from fermented dough, which contains a variety of wild bacteria. The fermented dough is used as a sample to prepare the dough extract, and the pure strain is obtained through dilution coating plate separation method. It is confirmed that the strain belongs to Saccharomyces cerevisiae. The identification method of the bacterial strain is as follows: 5.8S rRNA sequencing is used for identification, and the result shows that the sequence homology is greater than 99%, thus confirming that the isolated bacterial s...

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Abstract

The invention relates to a bacterium inhibiting feed and a preparation method and application thereof. The bacterium inhibiting feed comprises a basic ration and yeast essence, wherein the content ofbeta-glucan in the yeast essence is 25-50%, and the relative molecular weight of 99% or above of the beta-glucan is 3.0*10<5>-1.0*10<6>; and the content of mannan in the yeast essence is greater thanor equal to 20%, and the relative molecular weight of 99% or above of the mannan is 8.0*10<5>-2.0*10<6>. The yeast essence related in the feed disclosed by the invention is used as a single substanceto be added in the feed, the recommendation dosage is stable, and after in vitro bacterium inhibiting zone simulation and breeding animal in vivo experiment verification, the bacterium inhibiting feedshows good antibiotic replacement effects; an efficient feasible antibiotic replacement scheme can be provided for the animal breeding trade, besides, the defects that antibiotics have drug toleranceand are remained, and current antibiotic replacement products are bad in effects and lack stability are overcome, and the bacterium inhibiting feed has considerable future prospects.

Description

technical field [0001] The invention relates to the field of animal husbandry, mainly to the technical field of animal feed, in particular to an antibacterial feed and its preparation method and application. Background technique [0002] Feed antibiotics have the functions of improving feed digestion and utilization, promoting animal growth, improving body immunity, and reducing mortality. However, in recent years, the rate of drug-resistant strains caused by drug residues in animal products has continued to increase, the treatment of bacterial diseases has become more and more difficult, and the effective dose of antibiotics has continued to rise. Therefore, the development of new green and safe feed additives to replace or partially replace antibiotics in livestock and poultry farming is of great significance for alleviating drug residues in livestock and poultry products and drug resistance of pathogenic microorganisms, and ensuring food safety. [0003] At present, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/14A23K10/18A23K10/22A23K10/26A23K10/30A23K10/37A23K20/142A23K20/147A23K20/158A23K20/163A23K20/174A23K20/20A23K20/24A23K20/26A23K50/30A23L3/3571
CPCA23K20/163A23K10/18A23K10/14A23K10/30A23K10/37A23K20/158A23K10/22A23K20/147A23K20/20A23K20/26A23K10/26A23K20/24A23K20/174A23K20/142A23K50/30A23L3/3571A23V2002/00Y02P60/87
Inventor 蔡大亮俞学锋李知洪胡骏鹏吴晓峰谢智文黄鑫戴晋军陈中平聂琴
Owner 安琪酵母(柳州)有限公司
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