A method for collecting a large amount of pure eggs of Clonorchis sinensis

Abstract

The invention relates to the technical fields of life science, medicine and veterinary medicine, in particular to a method for collecting a large amount of pure eggs of Clonorchis sinensis. Step 1: Autoclave the normal saline, add double antibodies to the normal saline after autoclaving, and configure the concentration of double antibodies in the normal saline to be 100 μg / ml; Step 2: Collect adults, collect about 50 worms. The invention provides a method for collecting a large amount of pure eggs of Clonorchis sinensis. The method can not only collect a large amount of eggs of Clonorchis sinensis, but also prepare some ES antigens.

Description

technical field [0001] The invention relates to the technical fields of life science, medicine and veterinary medicine, in particular to a method for collecting a large amount of purified eggs of Clonorchis sinensis. Background technique [0002] In the field of parasitic disease diagnosis, such as clonorchiasis, the gold standard for clinical diagnosis is to extract and find parasite eggs from the host or host excrement. Currently, collecting eggs from the host’s feces is the most important detection method Method, in the prior art, detection method of worm's egg in feces comprises direct smear method, floating method, sedimentation method, nylon sieve elutriation method and improved Kato method (also known as Kato-Katz method) etc., the shortcoming of these methods is The number of eggs collected from feces is small, and there are many impurities, which is not conducive to microscope observation and later tests on eggs, such as DNA extraction from eggs, identification of i...

AI Technical Summary

Problems solved by technology

[0002] In the field of parasitic disease diagnosis, such as clonorchiasis, the gold standard for clinical diagnosis is to extract and find parasite eggs from the host or host excrement. Currently, collecting eggs from the host’s feces is the most important detection method Method, in the prior art, detection method of worm's egg in feces comprises direct smear method, floating method, sedimentation method, nylon sieve elutriation method and improved Kato method (also known as Kato-Katz method) etc., the shortcoming of these methods is The number of eggs collected from feces is small, and there are many impurities, which is not conducive to microscope observation and later tests on eggs, such as DNA extraction from eggs, identification of insect species and molecular biological detection methods of eggs in later tests. establish research