Lactobacillus casei capable of improving intestinal health and application thereof

A technology of Lactobacillus casei and intestinal health, applied in the field of microbiology, can solve the problems of lack of pertinence of Parabacteroides, hindering the treatment of Parabacteroides infection, etc., achieve good oligosaccharide utilization ability, improve intestinal health, and have great application prospects

Pending Publication Date: 2020-04-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the above-mentioned probiotics lack specificity to Parabacteroides. At present, there is still no probiotic strain that can significantly inhibit Parabacteroids in the human intestinal tract, which undoubtedly hinders the treatment of Parabacteroids infection

Method used

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  • Lactobacillus casei capable of improving intestinal health and application thereof
  • Lactobacillus casei capable of improving intestinal health and application thereof
  • Lactobacillus casei capable of improving intestinal health and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: Screening of Lactobacillus casei and identification of bacterial species

[0050] 1. Filter

[0051] The kimchi from Zhongxiang Town, Sichuan Province was used as a sample, and it was diluted 10-fold with sterile normal saline to 10 -6 , and then take 100 μL of dilution gradient to 10 -4 , 10 -5 , 10 -6 The dilutions of the MRS were coated on MRS solid medium, cultured at 37°C for 48 hours, and the colony morphology was observed and recorded; the colonies of different shapes on the MRS solid medium were picked for streak separation, and after culturing at 37°C for 48 hours, the colonies were picked again. Single colonies of different shapes on the MRS solid medium were streaked and separated until pure single colonies with the same shape were obtained; the pure colonies on the MRS solid medium were picked and inoculated into 5 mL MRS liquid medium, and incubated at 37°C for 18 h; 1 mL of bacterial liquid was placed in a sterile centrifuge tube, centrif...

Embodiment 2

[0054] Example 2: Cultivation of Lactobacillus casei

[0055] Lactobacillus casei CCFM1038 was added to MRS solid medium and cultured at 37°C for 48 hours, and the colonies were observed and found that the colonies were round, milky yellow, opaque with smooth edges.

Embodiment 3

[0056] Example 3: Tolerance of different Lactobacillus casei to simulated gastrointestinal fluids

[0057] Specific steps are as follows:

[0058] 1. Tolerance of different Lactobacillus casei to simulated gastric juice

[0059] Lactobacillus casei (Lactobacillus casei) CCFM1038, Lactobacillus casei (Lactobacillus casei) LC1, Lactobacillus casei (Lactobacillus casei) LC2, Lactobacillus casei (Lactobacillus casei) LC3, Lactobacillus casei (Lactobacillus casei) LC4 obtained in Example 1 , Lactobacillus casei (Lactobacillus casei) LC5, Lactobacillus casei (Lactobacillus casei) LC6 and Lactobacillus casei (Lactobacillus casei) LC7 were respectively inserted into the MRS liquid medium and cultured at 37 ° C for 18h, and the cells were collected by centrifugation. After washing with physiological saline, after washing, the cells were collected by centrifugation again, and the collected cells were resuspended in physiological saline containing 3 g / L pepsin with a pH of 3 (pH adjuste...

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PUM

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Abstract

The invention discloses lactobacillus casei capable of improving intestinal health and an application thereof, which belong to the technical field of microorganisms and medicines. The lactobacillus casei CCFM1038 screened by the invention can improve the intestinal health, which is specifically reflected in that (1) the lactobacillus casei CCFM1038 can grow by taking fructo-oligosaccharide, xylooligosaccharide and galactooligosaccharide as a unique carbon source and metabolize to produce acid, and has good oligosaccharide utilization capability; (2) various short-chain fatty acids such as acetic acid, propionic acid and butyric acid can be produced through in-vitro metabolism; and (3) the relative abundance of Paraboroids in intestinal tracts can be reduced, so that the lactobacillus caseiCCFM1038 has a huge application prospect in the preparation of products (such as foods, medicines or health-care products) for improving the health of intestinal tracts.

Description

technical field [0001] The invention relates to a Lactobacillus casei capable of improving intestinal health and its application, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] More than 99% of the microorganisms in the human gut are bacteria, and these huge numbers of bacteria can be roughly divided into three categories: beneficial bacteria, harmful bacteria and neutral bacteria. Among them, beneficial bacteria, also known as probiotics, are indispensable elements of human health. They can synthesize various vitamins, participate in the digestion of food, promote intestinal peristalsis, inhibit the growth of pathogenic bacteria, and decompose harmful and toxic substances. ; Harmful bacteria, once the number grows out of control, it will cause various diseases, produce harmful substances such as carcinogens, or affect the function of the immune system; Neutral bacteria, that is, bacteria with dual functi...

Claims

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Application Information

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IPC IPC(8): A23L33/135A61K35/747A61P1/00
CPCA23L33/135A61P1/00A23V2002/00A61K2035/115A23V2400/125A23V2200/3204
Inventor 翟齐啸陈卫田丰伟陆文伟吉梦馨沈旭丹于雷雷赵建新张灏
Owner JIANGNAN UNIV
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