96 results about "Gastrointestinal fluids" patented technology

Provided is an antibacterial peptide having an amino acid sequence X1KRFKKFFX2KLKKWV-NH2, wherein X1 is selected from any one or of amino acids A, C, D, E, F, G, H, K, L, N, M, P, Q, R, S, I, V, W, Y, and T or is absent; and X2 is an amino acid having an aromatic side chain or an amino acid having an alkaline side chain. In view of defects such as poor gastrointestinal fluid stability of antibacterial peptides in the prior art, a brand-new sequence design scheme and preparation method for an antibacterial peptide are provided. By means of a complete or partial D-amino acid substitution or structural derivatization of a peptide sequence, a series of antimicrobial peptides having stronger antibacterial activity and gastrointestinal stability as compared with the prior art are obtained, such that the antibacterial peptides have a wider application range and higher development potential value.

The invention relates to the technical field of antibacterial drugs, in particular to an antibacterial peptide which has an amino acid sequence X1KRFKKFFX2KLKKWV-NH2, in which X1 is selected from any one or deletion of amino acids A, C, D, E, F, G, H, K, L, N, M, P, Q, R, S, I, V, W, Y and T; and X2 represents an amino acid having an aromatic side chain or an amino acid having a basic side chain. Aiming at the defects of poor gastrointestinal fluid stability and the like of the antibacterial peptide in the prior art, the invention provides a brand new antibacterial peptide sequence design scheme and a preparation method thereof, and the peptide sequence is subjected to full D-type amino acid or partial D-type amino acid replacement or structure derivation; compared with the prior art, a series of antibacterial peptides with higher antibacterial activity and gastrointestinal fluid stability are obtained, so that the antibacterial peptides have wider application range and higher potential development value.

The invention discloses a lactobacillus rhamnosus strain with antiallergic activity and a preparation method thereof, the strain is lactobacillus rhamnosus Lrn-PG-06, the classification name of the strain is Lactobacillus rhamnosus, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.3077, the preservation time is March 21, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.3077, and the preservation number is CGMCC No.3077. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The lactobacillus rhamnosus Lrn-PG-06 strain is high in acid resistance, alkali resistance and cholate resistance, high in simulated gastrointestinal fluid resistance and high in oxidation resistance, has the advantage of resisting allergy, and solves the problems that at present, allergic diseases are mainly treated by drugs or are prevented from being in contact with allergens in the environment and food, but due to the fact that the drugs are large in toxic and side effects, the allergens cannot be in contact with the allergens in the environment and food. And allergens causing anaphylaxis are difficult to determine, so that allergic diseases are difficult to treat or prevent.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium animalis for relieving eye allergy and application of the bifidobacterium animalis. The invention provides an application of bifidobacterium animalis subsp. Lactis in preparation of a product for relieving eye allergy, the bifidobacterium animalis subsp. Lactis is Def +, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35798. The Bifidobacterium animalis subsp. Lactis Def < + > provided by the invention can relieve the eye itching symptom, relieve red and swollen eyelids, reduce conjunctiva and cornea injury, relieve eye discomfort, relieve globular conjunctivitis cell infiltration, improve corneal epithelium thickening lesion, improve the anti-inflammatory ability and improve allergic conjunctivitis, keratitis and blepharitis marginata in a targeted manner. The eye drops are high in safety and resistant to gastrointestinal fluid, and a safe and long-acting scheme is provided for eye allergic diseases.

The invention relates to the technical field of biological medicine, and discloses bifidobacterium adolescentis YF-01 and application thereof in fermented milk. The bifidobacterium adolescentis YF-01 is separated from adult excrement and is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20251308. Experiments prove that the bifidobacterium adolescentis YF-01 has good gastrointestinal fluid tolerance, the survival rate of the bifidobacterium adolescentis YF-01 can reach 90.1% after simulated gastric fluid treatment for 3 h, and the survival rate of the bifidobacterium adolescentis YF-01 can reach 72% after simulated intestinal fluid treatment for 4 h The strain has the self-aggregation rate of 88.83% and the hydrophobicity of 79.41%, has remarkable scavenging capacity on DPPH free radicals and hydroxyl free radicals, shows good antioxidant activity and adhesion capacity, and provides a theoretical basis for application of the strain in fermented milk. The strain and metabolites thereof can be used for preparing food, medicines or health care products capable of regulating intestinal flora and enhancing oxidation resistance, and have remarkable application potential in development of functional fermented milk products.

This invention discloses a probiotic microcapsule and its preparation method, belonging to the field of microcapsule preparation technology. The preparation method includes the following steps: (1) dissolving Sophora japonica extract in water, stirring, adding sodium alginate, and stirring again to obtain a wall material solution; (2) centrifuging and washing the activated probiotics, preparing a bacterial suspension, mixing it with the wall material solution obtained in step (1), stirring, and obtaining a wall material bacterial suspension; (3) adding the wall material bacterial suspension obtained in step (2) to a calcium chloride solution and letting it stand, then adding the formed gel to a chitosan solution, stirring, washing, and obtaining the final product. The preparation process of this invention is simple and convenient, and the raw materials are inexpensive and easy to obtain, which can effectively reduce the production cost of microcapsules, facilitate large-scale industrial production and application promotion, effectively improve the biocompatibility, biodegradability, mechanical strength and encapsulation rate of microcapsules, and enhance the storage stability and resistance to gastrointestinal fluid stress of microcapsules.

According to the invention, the traditional fermented koumiss in Xinjiang is collected, two novel multifunctional probiotic strains, namely, the lactobacillus plantarum SR6 GDMCC NO.66817 strain and the enterococcus faecalis SM5 GDMCC NO.66816 strain with good compatibility are separated and screened out, and the two strains have the characteristics of reducing blood fat, reducing cholesterol, removing triglyceride, resisting oxidation and antagonizing pathogenic bacteria, and have the characteristics of good cholate and gastrointestinal tract tolerance, high survival rate and good safety. The growth characteristics and compatibility of the two strains are improved; the inhibition rate of cholesterol esterase and pancreatic lipase; the method comprises the following steps: evaluating the triglyceride removal rate, antioxidant activity, pathogenic bacterium resistance, artificially simulated gastrointestinal fluid tolerance, strain drug resistance and safety, compounding lactobacillus plantarum SR6 and enterococcus faecalis SM5 strains according to a ratio of 1: 1 to prepare a freeze-dried bacterial agent, and keeping good survival rate (pgt; 0.05) and functional characteristics, the strain can be used as a functional direct vat set starter and is directly applied to production of fermented dairy products, and the biological activity of food is improved.

The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.

Embodiments of devices and methods for sampling gastrointestinal fluids and assessing gastrointestinal function using a device comprising a sampling tube are provided.

The invention belongs to the technical field of microorganisms, and relates to a bifidobacterium breve strain capable of relieving constipation, the strain is bifidobacterium breve Bb-PG-14, the classification name of the strain is Bifidobacterium breve, the preservation number is CGMCC No.31861, the preservation time is September 4, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, and the preservation number is CGMCC No.31861. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is separated and purified from excrement of healthy babies, has relatively strong acid resistance, alkali resistance, cholate resistance and artificial simulated gastrointestinal fluid tolerance, and has the characteristics of probiotics.

The invention belongs to the technical field of surgical instruments, and particularly relates to a gastrointestinal fluid decompression device for gastrointestinal surgery, which comprises a shell, a negative pressure assembly, a cleaning assembly and a switching mechanism, the switching mechanism drives the negative pressure assembly and the cleaning assembly to be in butt joint with the butt joint opening in a negative pressure mode and a cleaning mode correspondingly and form negative pressure and release cleaning liquid in a pipeline connected with the butt joint opening correspondingly, the negative pressure assembly is a negative pressure peripheral pump, the cleaning assembly is a liquid conveying screw pump, the switching mechanism comprises a gear ring and a motor, the gear ring is rotationally arranged in the shell, and the motor is arranged in the shell. A transmission gear is arranged on an output shaft of the motor and meshed with the outer side of the gear ring, the inner side of the gear ring is meshed with a rotating shaft of the negative-pressure peripheral pump and a transmission rod of the liquid conveying screw pump, and positive and negative rotation of the gear ring is used for driving the negative-pressure peripheral pump and the liquid conveying screw pump to be switched to the butt joint position of the butt joint opening and driving the rotating shaft and the transmission rod to rotate. The negative pressure can be provided and the pipeline can be cleaned by switching positions.

The application discloses a plant lactobacillus strain and application thereof, and belongs to the technical field of microorganisms. The preservation number of the strain is CGMCC No. 33864. The strain has good tolerance to gastrointestinal juice and bile salts and has no hemolytic activity. Concentrated cell-free supernatant prepared from the strain can inhibit salmonella, including inhibiting the growth of salmonella, killing bacteria, inhibiting or removing biofilm. Animal experiments show that the strain and the concentrated cell-free supernatant thereof can prevent salmonella infection, alleviate weight loss caused by infection, maintain intestinal barrier integrity, reduce the level of pro-inflammatory factors, alleviate tissue damage, improve the expression of colon tight junction proteins and mucin, promote the generation of short-chain fatty acids, and restore the intestinal flora structure. The application further provides a microbial preparation containing the strain and / or the concentrated cell-free supernatant thereof. The strain and the microbial preparation can be used for preparing products for resisting salmonella, preventing salmonella infection or improving intestinal health.

This invention discloses a method for preparing piperine starch microcapsules with sustained-release properties, belonging to the field of food processing technology. Using piperine as the core material, oleic acid as the oil phase carrier, and PG 2000 type octenyl succinate starch ester as the wall material, sustained-release piperine microcapsules are prepared through oil phase preparation, aqueous phase preparation, high-speed shearing, high-pressure homogenization, and spray drying. The microcapsules prepared by this invention have high encapsulation efficiency, small and uniformly distributed reconstituted particle size, and a stable system that is not prone to sedimentation. This allows for the slow and continuous release of piperine in simulated gastrointestinal fluid, significantly improving its poor water solubility, low stability, and low bioavailability. The resulting product can be widely used in the food, health product, and pharmaceutical fields.

The application discloses a plant Lactobacillus rhamnosus microcapsule and a preparation method and application thereof, and the plant Lactobacillus rhamnosus is embedded by adopting a low-temperature spray drying process with lily starch, citrus pectin and whey protein as wall materials, and mainly includes the following steps: preparation of a bacterial suspension, preparation of a wall material solution, preparation of a microcapsule solution and low-temperature spray drying for preparing the microcapsule. The preparation technology of the stable encapsulated plant Lactobacillus rhamnosus microcapsule provided by the application has the advantages that the viable bacterial count in the microcapsule is high after spray drying, the microcapsule has strong gastrointestinal fluid resistance, is stable in storage, and can effectively solve the problem that the bacterial activity of the plant Lactobacillus rhamnosus and products thereof is reduced or even inactivated in the production and processing, storage and transportation and in-vivo digestion process; the prepared microcapsule can be used as a probiotic bacterial agent and applied to foods such as cheese, yogurt, ice cream, milk, chocolate, lactic acid bacterial beverages and baked products, and helps to promote the healthy development of the probiotic industry, and has obvious economic and social benefits.

A Limosilactobacillus fermentum strain capable of alleviating a hangover and the use thereof. The strain is at least one strain of Limosilactobacillus fermentum ibiome020, Limosilactobacillus fermentum ibiome021 and Limosilactobacillus fermentum ibiome022 respectively deposited with deposit numbers of CCTCC NO: M 2024188, CCTCC NO: M 2024222 and CCTCC NO: M 2024189. The Limosilactobacillus fermentum is a natural bacterium derived from the intestine of a healthy human and has high safety; can alleviate chronic / acute alcoholism, alcoholic intestinal damage or alcoholic liver damage; can accelerate the metabolism of ethanol and / or acetaldehyde, and delay the onset of intoxication; can shorten the sobering-up time; can reduce the mortality caused by drinking alcohol; can reduce the liver index; can lower the level of AST and / or ALT in serum; can alleviate discomfort caused by ethanol and / or acetaldehyde, and mitigate symptoms such as dizziness, headache, nausea and vomiting, and hangover after drinking alcohol; can ameliorate oxidative stress caused by alcohol, and increase the level of GSH in the liver; and also has excellent resistance to gastrointestinal fluid and bile salt, and can adapt to the complex environment within the body.

The present invention provides a gastrointestinal fluid decompressor, comprising a collection box, a suction assembly, wherein the suction assembly is mounted on the upper portion of the collection box; a filter screen connected to the side wall of the inner cavity of the collection box, and used to filter and process the gastrointestinal fluid; and a crushing mechanism, wherein the crushing mechanism comprises a crushing mesh cylinder, wherein two symmetrically distributed crushing mesh cylinders are connected to the bottom of the filter screen, a crushing assembly is disposed inside the crushing mesh cylinder, and a lifting assembly is disposed outside the crushing assembly. The present invention utilizes the filter screen to filter the gastrointestinal fluid, utilizes the crushing assembly to crush solid tissue in the filtered gastrointestinal fluid, and utilizes the lifting assembly to lift the crushed solid tissue filtered by the crushing mesh cylinder and send it into the crushing assembly for further crushing, and repeats the process multiple times until all solid tissue is crushed, thereby preventing larger solid tissue from clogging the discharge port of the collection box or other processing equipment, and facilitating subsequent processing of the gastrointestinal fluid.

The invention discloses a quercetin nano-vesicle complex as well as a preparation method and application thereof. The preparation method of the nano-vesicles comprises the following steps: separating and purifying extracellular vesicle-like nano-particles from cow milk by combining differential centrifugation with an EDTA precipitation method; quercetin is efficiently loaded in a vesicle nanostructure by adopting an ultrasonic-assisted co-incubation technology, and a drug loading system is characterized through particle size analysis, Zeta potential determination and a transmission electron microscope. The natural lipid bilayer structure of the milk-derived extracellular vesicles is creatively utilized, the constructed quercetin nano-vesicle complex shows excellent stability in simulated gastrointestinal fluid, and the average encapsulation efficiency can reach 60%. In-vitro and in-vivo experiments prove that the nano vesicles can efficiently remove senescent cells. The invention has a wide clinical application prospect in the field of anti-aging and inflammation-related disease treatment.

The invention belongs to the technical field of microorganisms, and relates to a lactobacillus paracasei Lpc-14 strain with functions of resisting oxidation and promoting gastrointestinal motility and a preparation method of the lactobacillus paracasei Lpc-14, the lactobacillus paracasei is classified and named as Lactobacillus paracasei, the preservation number of the lactobacillus paracasei is CGMCC No. 29666, the preservation time is January 18, 2024, and the preservation number of the lactobacillus paracasei Lpc-14 is CGMCC No. 29666. The preservation unit is China General Microbiological Culture Collection Center, and the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is separated and purified from naturally-fermented Chinese sauerkraut fermentation liquor, has the capacity of resisting oxidation and promoting gastrointestinal motility of mice with constipation and improving secretion of gastrointestinal regulating peptides, further has the capacity of resisting acid and alkali and resisting artificial simulated gastrointestinal fluid and bile, and has the characteristics of probiotics.

The invention relates to the technical field of gastrointestinal decompression, and particularly discloses a gastrointestinal fluid decompression device for gastrointestinal surgery, which comprises a bag-shaped container and a connecting pipe, the top of the bag-shaped container is fixedly connected with an upper cover, and the bag-shaped container and the upper cover are internally and fixedly connected with a fixing pipe; firstly, the intubation tube is installed at one end of the connecting tube, the bearing assembly is installed at the top of the bag-shaped container through the fixing tube, the connecting tube is inserted into the bearing assembly through the inserting assembly during use, and the inserting assembly is rotated to enable the intubation tube to be communicated with a movable sleeve discharge opening; the rotary insertion pipe is continuously pressed, the limiting block drives the sealing sleeve to rotate, the insertion pipe is longitudinally fixed, meanwhile, the movable sleeve is driven to be positioned and fixed along the guide block and the guide groove, longitudinal limiting of the rotary insertion pipe is relieved during disassembly, the insertion pipe and the movable sleeve move upwards under the elastic force effect of the reset assembly, and the movable sleeve seals the bottom of the mounting base; the intubation tube is continuously rotated and lifted upwards, the movable sleeve is reset and fixed, the sealing sleeve moves upwards, the top of the bag-shaped container and the port of the connecting tube are synchronously sealed, liquid leakage and dripping are avoided, and the pollution risk is reduced.

The invention discloses an anthocyanin ingredient containing milk fat globules and a preparation method thereof.The preparation method comprises the specific steps that the milk fat globules are extracted from fresh raw milk, anthocyanin and the milk fat globules are added into deionized water to be emulsified, and the anthocyanin emulsion type ingredient containing the milk fat globules is obtained; wherein anthocyanin in the ingredients and a fat membrane of the milk fat globules form a specific structure, and the specific structure is rich in long-chain unsaturated fatty acid and medium-chain fatty acid in a specific ratio of 1: (7-10) after being digested by in-vitro simulated gastrointestinal fluid. The anthocyanin ingredient containing the milk fat globules is a novel stable emulsion taking the natural milk fat globules as an anthocyanin carrier, is particularly suitable for being used as an ingredient of nutritional and healthy food, and is wide in application range. And the preparation method adopts a probe type ultrasonic combined high-speed shearing technology, so that the obtained emulsion is better in dispersity and stability, simple, efficient, green and safe.

The invention discloses an intestinal micro-emulsified targeted release type xanthoceras sorbifolia bunge oil soft capsule and a preparation method thereof, and belongs to the technical field of functional food and health-care product preparations. The soft capsule is composed of a content containing low-temperature squeezed xanthoceras sorbifolia bunge oil and a self-microemulsion delivery system, and a gastrointestinal fluid response type capsule shell. The preparation method comprises the steps of delivery system preparation, capsule shell preparation, capsule forming, quality detection and the like. Intestinal targeted delivery, controllable disintegration and intestinal in-situ emulsification core technologies are integrated to form a synergistic effect, so that active ingredients such as nervonic acid safely pass through the stomach and are accurately released in the intestinal tract to form a nano-scale microemulsion, the bioavailability is improved from less than 20% to more than or equal to 60%, and the nano-scale microemulsion is high in compatibility with an existing production line, controllable in quality, low in industrialization cost and suitable for industrial production. The xanthoceras sorbifolia bunge oil can be promoted to be upgraded to a high-valued functional preparation.

The invention belongs to the technical field of food microorganisms, and particularly relates to a lactobacillus paracasei BHP06 with an anti-allergic function. The preservation number of the Lactobacillus paracasei BHP06 is GDMCC (China General Microbiological Culture Collection Center) No: 66270, and the preservation number of the Lactobacillus paracasei BHP06 is CGMCC No: 66270. The lactobacillus paracasei strain provided by the invention has good gastrointestinal tract reverse environment resistance and has good survival ability in simulated gastrointestinal fluid; good self-aggregation capability and hyaluronidase inhibition capability are realized. By establishing a degranulation model of RBL-2H3 cells, it is found that the strain can inhibit mast cell degranulation and relieve allergy; by establishing an RAW264.7 cell inflammation model, the strain disclosed by the invention is found to be capable of inhibiting allergic inflammatory reaction; by establishing an OVA (ovalbumin)-induced BALB / c mouse food allergy model, the strain disclosed by the invention is found to be capable of improving the anti-allergy ability of animals and exerting the activity of relieving food allergy.

The invention relates to the field of gastrointestinal surgical nursing, and discloses a gastrointestinal fluid decompression device for gastrointestinal surgical nursing, which comprises a tank body, a negative pressure bottle body is arranged in the tank body, a pressure supplementing assembly is arranged on one side of the tank body, and a partition assembly is arranged above the pressure supplementing assembly. Through the deformation linkage design of the negative-pressure self-adaptive pressure supplementing assembly and the elastic negative-pressure bottle, real-time monitoring and dynamic compensation of the negative pressure in the negative-pressure bottle are achieved, the pressure supplementing strength can be automatically adjusted according to the springback unfolding degree of the negative-pressure bottle, the problems that the negative pressure of a traditional negative-pressure bottle is continuously attenuated, and pressure fluctuation is large are fundamentally solved, and the service life of the negative-pressure bottle is prolonged. And the whole gastrointestinal drainage process is kept in a stable, uniform and effective negative pressure state. The stable drainage pressure can avoid stimulation to gastrointestinal tracts of patients caused by sudden strong and weak suction, uncomfortable symptoms such as abdominal distension, nausea and reflux are remarkably reduced, drainage sufficiency of gastrointestinal fluid is improved, hydrops retention and related complication risks are reduced, and safer and more comfortable decompression guarantee is provided for postoperative recovery of the patients.

The application provides a lactobacillus gasseri strain A21120 with antioxidant, anti-inflammatory and antitumor functions, and a probiotic agent and application thereof, and belongs to the technical field of functional microorganisms. The strain A21120 is obtained by screening from intestinal feces of a centenarian healthy old person, and is identified by morphology and molecules and belongs to lactobacillus gasseri. The strain has good resistance to gastrointestinal fluid and bovine choline capacity, and has the potential to colonize the intestinal tract. The lactobacillus gasseri strain A21120 provided by the application has bacteriostatic, anti-inflammatory, antioxidant and antitumor activities, and provides a new way for the treatment of clinical related diseases, the development of drugs and the preparation of in vitro preparations or daily necessities.

The invention discloses lactobacillus rhamnosus and application thereof, the lactobacillus rhamnosus is named as lactobacillus rhamnosus, the preservation number of the lactobacillus rhamnosus is CGMCC (China General Microbiological Culture Collection Center) No.35978, and the lactobacillus rhamnosus is preserved in the China General Microbiological Culture Collection Center on September 18, 2025. The strain has excellent alcohol tolerance and can survive in a 15% ethanol environment, and the degradation rate of the strain to a 10% ethanol solution in vitro reaches 17% or above; meanwhile, the strain has outstanding gastrointestinal fluid tolerance, and the survival rate of the strain treated in simulated gastric fluid with the pH value of 2.5 for 2 hours exceeds 93%. The strain and the fermentation product thereof also show remarkable antioxidant activity, and can effectively remove DPPH free radicals. The invention further provides application of the bacterial strain in preparation of products for dispelling effects of alcohol, protecting liver and resisting oxidation, and fermentation liquor, bacterial suspension and related compositions containing the bacterial strain, and the bacterial strain can be widely applied to the fields of functional foods, medicines and health-care products.

The invention discloses lactobacillus crispatus CZ02 resistant to gastrointestinal digestion, a high-density fermentation method thereof and application of the lactobacillus crispatus CZ02 in female reproductive health, and belongs to the technical field of microorganisms. On one hand, the invention provides the lactobacillus crispatus CZ02 resistant to gastrointestinal digestion, and on the other hand, the invention provides application and a high-density fermentation method of the lactobacillus crispatus CZ02. The lactobacillus crispatus CZ02 provided by the invention has a relatively high survival rate in a low pH value, and can resist a strong acid environment. The lactobacillus crispatus disclosed by the invention has good acid resistance, artificial gastrointestinal fluid tolerance and cholate tolerance, and the lactobacillus crispatus disclosed by the invention has relatively strong tolerance in different polar environments, has better stability in a hydrophobic or high polar environment, and is suitable for being planted in intestinal tracts. The lactobacillus crispatus CZ02 disclosed by the invention can migrate from intestinal tracts to vaginas for colonization, can inhibit growth of pathogenic microorganisms and regulate flora balance in the vaginas, so that related diseases caused by vaginal flora disorder can be prevented and / or treated.

The present disclosure relates to a device for collecting gastrointestinal fluids / particles or samples. The present disclosure also relates to a process of collecting gastrointestinal fluids / particles or samples by the ingestible device. The ingestible device of the present disclosure is designed to non-invasively collect gastrointestinal particles, including biomolecules and components of the residing gut microbiome, with precise spatial resolution along the gastrointestinal (GI) tract. Upon deployment, the device's design and / or actuation mechanism facilitates the capture of gastrointestinal fluids and associated particles within those fluids. These samples are securely stored within the device until its natural egestion, after which the collected material can be subjected to further bio / chemical analyses.

The invention discloses a probiotic embedding method, and belongs to the technical field of probiotic preparations. The preparation method comprises the following steps: mixing and dissolving pullulan and whey protein to obtain a first spinning solution; mixing the first spinning solution with a probiotic resuspension to obtain a first spinning solution containing probiotics; zein and ethyl cellulose are mixed and dissolved to obtain a second spinning solution; the first spinning solution containing the probiotics and the second spinning solution are subjected to electrostatic spinning to form a double-layer structure to complete embedding of the probiotics, so that the survival rate of the probiotics is increased, and the tolerance, viability and storage stability of the probiotics for resisting gastrointestinal fluid and cholate are also improved; the adopted electrostatic spinning technology has the advantages of being high in production efficiency, easy to operate, mild in condition, low in cost and the like, and the raw materials are all food-grade materials and are non-toxic, high in safety and wide in application range.

This invention relates to the application of a melanin-catalase nanocomposite (MAC) targeting intestinal mucosal neutrophils in the preparation of drugs for treating inflammatory bowel disease, belonging to the field of novel applications of nanomedicine. It addresses the challenges of catalase oral delivery being easily degraded and inactivated by gastrointestinal proteases and difficult to precisely target neutrophils. This invention is the first to discover a MAC prepared by in-situ polymerization of dopamine on the surface of catalase, which can be used for the treatment of inflammatory bowel disease. This nanocomposite, through oral delivery, precisely targets intestinal mucosal infiltrating neutrophils and maintains structural stability in the gastrointestinal fluid environment, effectively protecting catalase activity. It actively targets intestinal mucosal infiltrating neutrophils through the high affinity of melanin for dopamine D2 receptors, synergistically exerting a triple effect of hydrogen peroxide decomposition, hypochlorous acid scavenging, and iron ion chelation, effectively inhibiting the formation of extracellular traps in neutrophils.

The invention relates to the field of food, in particular to probiotic characteristics of lactobacillus salivarius JCF5 and a method for preparing Jersey yogurt through synergistic fermentation of the lactobacillus salivarius JCF5. The strain provided by the invention is named as Lactobacillus salivarius JCF5, and the Latin is Ligillactobacillussavarius. The invention further discloses a preparation method of the strain. The strain provided by the invention has the advantages that the strain is named as Lactobacillus salivarius JCF5; the strain is obtained by randomly selecting six newly-born Jersey and six Jersey-and-cattle hybrid calves with good health conditions from a pasture in Heilongjiang province, collecting fetal manure of the Jersey and six Jersey-and-cattle hybrid calves after the Jersey and cattle hybrid calves are born, and separating manure samples after the Jersey and cattle hybrid calves eat colostrum. The water-holding capacity and whey precipitation rate of the Jersey yoghourt can be remarkably improved, and the viscosity of the Jersey yoghourt can be increased. In addition, the strain has better acid resistance, cholate resistance and simulated gastrointestinal fluid resistance, does not show hemolysis capacity and gelatinase activity, has stronger antibacterial capacity and antibiotic sensitivity, and has the characteristics of probiotics. The application potential of the strain in fermenting the Jersey yogurt is fully explored, the quality of the Jersey yogurt is expected to be improved by changing the strain proportion of the leavening agent sold in the market, and a new leavening agent is provided for fermentation of the Jersey yogurt.