Microencapsulated Enterococcus faecium live bacterium preparation and its preparation method

A technology of Enterococcus faecium and live bacteria preparations, which is applied in the field of microbial fermentation engineering, can solve the problems of high cost of freeze-drying technology and the easy formation of honeycombs in microcapsules, and achieve improved feed utilization, good product shape, and strong stress resistance Effect

Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of freeze-drying technology is high, and the microcapsules are easy to form honeycomb after freeze-drying, which cannot give full play to the advantages of microcapsules

Method used

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  • Microencapsulated Enterococcus faecium live bacterium preparation and its preparation method
  • Microencapsulated Enterococcus faecium live bacterium preparation and its preparation method
  • Microencapsulated Enterococcus faecium live bacterium preparation and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method of microencapsulated Enterococcus faecium live bacteria preparation comprises the following steps:

[0019] 1. Optimization of fermentation medium:

[0020] Enterococcus faecium (Enterococcus faecium) strains were purchased from the China Common Microorganism Culture Collection and Management Center, strain number: 1.2334, and 10 factors affecting the fermentation medium were evaluated by the Plackett-Burman design method, and those with significant effects were screened out. 3 ingredients: peptone, beef extract, diammonium citrate. The steepest climbing path is used to approach the maximum yield response area of ​​bacteroids, and the optimal level combination of the main influencing factors is determined by response surface analysis. The optimized medium composition is as follows: Enterococcus faecium fermentation medium:

[0021]

[0022]

[0023] Add the above ingredients into 1000mL distilled water, heat to dissolve, adjust the pH to 6.2...

Embodiment 2

[0033] Taking the free Enterococcus faecium preparation without microencapsulation treatment as a control, the tolerance of microencapsulated Enterococcus faecium solid products to high temperature, storage and transportation, and gastric acid and bile in gastrointestinal fluids was evaluated. The test data was verified by Microsof t After preliminary sorting by Excel, Originpro7.5 was used to conduct analysis of variance and multiple comparisons, all expressed as "average ± standard deviation", with P < 0. 01 (very significant difference) and P < 0. 05 (significant difference) as Criteria for judging the significance of the difference.

[0034] 2. 1 Resistance to high temperature

[0035] Take an appropriate amount of solid products of microencapsulated Enterococcus faecium, bathe them in water for 0, 2, 4, 6, 8 and 10 minutes at 80°C respectively, and cool them in running water. 3 replicates per treatment. Calculate the number of live bacteria per gram of product by plate ...

Embodiment 3

[0046] In order to verify the effect of microencapsulated Enterococcus faecium preparation on the growth performance and immunity of fattening pigs, 110 fattening pigs with a body weight of about 68 kg were selected in the experimental group and the control group, and 1.5% microencapsulated Enterococcus faecium preparation was added to the experimental group. The control group was not added, and other experimental conditions remained unchanged. The experimental results are shown in Table 2:

[0047] Table 1 Analysis of experimental results of microencapsulated Enterococcus faecium preparations promoting the growth of fattening pigs

[0048]

[0049] It can be seen from Table 1 that the average daily weight gain of the test group was 77.4g more than that of the control group, and the feed-to-meat ratio decreased from 3.06 in the control group to 2.72. Therefore, the microencapsulated Enterococcus faecium preparation can effectively improve feed utilization and maintain intest...

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Abstract

The invention relates to a preparation method of a microencapsulated Enterococcus faecium live bacterium preparation and application of the preparation in breeding. The method employs a technological process of ''fermentation-coating-fermentation''. By adding 2g/L calcium chloride to a fermentation medium and conducting drying at 45DEG C, a microencapsulated Enterococcus faecium solid product with good sphericity and uniform size can be obtained, and its viable count can reach 11.57lgCFU/g. Compared with free Enterococcus faecium solid products, the microencapsulated Enterococcus faecium live bacterium preparation has stronger ability to tolerate a high temperature of 80DEG C and a simulated gastrointestinal fluid. After storage for 2 months under a normal temperature condition, the viable count basically has no change. The microencapsulated Enterococcus faecium live bacterium preparation has a simple pre-fermentation coating preparation process, good product appearance, strong stress resistance, high stability, and high encapsulation efficiency. Being use as a high activity microecological agent for feeding in actual production, the microencapsulated Enterococcus faecium live bacterium preparation can inhibit propagation of intestinal spoilage bacteria and pathogenic bacteria and intestinal infection, reduce the diarrhea rate, promote digestive absorption of nutrients, enhance immunity, improve feed intake and feed conversion rate, as well as lower feed-meat ratio.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a preparation method in which a liquid medium is fermented by Enterococcus faecium and then coated with microcapsules and its application in aquaculture. Background technique [0002] Enterococcus faecium (Enterococcus faecium) is a normal flora in the intestinal tract of humans and animals, and is also an important lactic acid bacterium. It has good intestinal adhesion ability, can produce lactic acid and some antibacterial substances, inhibit intestinal spoilage bacteria, The reproduction of germs and intestinal infection can reduce the rate of diarrhea, promote the digestion and absorption of nutrients, enhance immunity, increase feed intake and feed conversion rate, and promote animal health. It is the preferred probiotic strain for animal husbandry production and food industry one. Compared with Bifidobacterium and Lactobacillus, which are strictly anaerobi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04A23K1/16A23K1/18C12R1/01
Inventor 李爱科李本涛王从峰何宏轩夏新界佘锐萍李刚印遇龙李帅伟
Owner GUANGZHOU GLAM BIOTECH
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