Method for detecting endogenous hormones in pinus massoniana branches

An endogenous hormone, masson pine technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as cumbersome detection procedures, and achieve the effects of solving cumbersome detection procedures, simple procedure steps, and saving space.

Inactive Publication Date: 2020-04-21
GUIZHOU MINZU UNIV
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  • Abstract
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Problems solved by technology

[0004] In order to solve the technical problem that the existing endogenous hormone assay method in the above-mentioned background technology has

Method used

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  • Method for detecting endogenous hormones in pinus massoniana branches
  • Method for detecting endogenous hormones in pinus massoniana branches
  • Method for detecting endogenous hormones in pinus massoniana branches

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Embodiment 1

[0038] A method for detecting endogenous hormones in pine branches, comprising the following steps:

[0039] A, get 3g masson pine branches and drop into liquid nitrogen and grind into powder, then add the massfraction of 45g and be 80% methyl alcohol, obtain mixed solution;

[0040] B. Add 0.48g of vitamin C to the mixed solution in step A and place it at 3-5°C for 2 hours, then centrifuge to obtain 3.2g of precipitate;

[0041] C. Take the precipitate in step B and add 32g of methanol, then centrifuge and take the supernatant, and pass the supernatant through polyvinylpolypyrrolidone PVPP column and diethylaminoethyl cross-linked dextran gel successively After the column is filtered, take the filtrate;

[0042] D. Dry the filtrate in step C, add analytical pure methanol to dilute to 1mL and use a disposable syringe to collect the dilute solution, then filter it with a 0.22μm microporous membrane and use high performance liquid chromatography-mass spectrometry The content o...

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Abstract

The invention relates to the field of determination of plant endogenous hormones, in particular to a method for detecting endogenous hormones in pinus massoniana branches. The method comprises the following steps: putting pinus massoniana branches into liquid nitrogen, grinding the branches into powder, and adding methanol to obtain a mixed solution; adding vitamin C into the mixed solution, keeping the solution standing in an environment of 3-5 DEG C, and carrying out centrifuging to obtain a precipitate; then adding methanol into the precipitate, then carrying out centrifuging, taking supernatant, filtering the supernatant through a polyvinylpyrrolidone (PVPP) column and a diethylaminoethyl cross-linked dextran gel column in sequence, and taking a filtrate; and finally, blow-drying the filtrate, adding analytically pure methanol to a constant volume, collecting the constant volume solution by using a disposable syringe, carrying out filtering by using a 0.22 <mu>m microporous filtermembrane, and analyzing and detecting by using high performance liquid chromatography-mass spectrometry to obtain the content of endogenous hormones in the pinus massoniana branches. The method is simple in procedure steps, the recovery rate of the detected endogenous hormone is high, and a detection result is accurate.

Description

technical field [0001] The invention relates to the field of determination of plant endogenous hormones, in particular to a method for detecting endogenous hormones in pine pine branches. Background technique [0002] Endogenous hormones refer to hormones synthesized by the plant itself. Because plant growth regulators (artificially synthesized hormone analogs) are often used in production practice to distinguish them from these exogenous hormones, the plant's own hormones are called endogenous hormones. Masson pine (Pinus massoniana) is the main tree species of artificial forests and natural forests in Southwest my country. It is also the most important timber tree species in southern China and the pioneer tree species of barren mountain afforestation. It has a wide distribution range, fast growth speed and strong adaptability. Characteristics, is a typical tree species of ectomycorrhizal. [0003] Endogenous hormones play an important role in regulating the growth and dev...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/74
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N30/74G01N2030/324
Inventor 王艺杨凯来谢豪田珊
Owner GUIZHOU MINZU UNIV
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