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Method for removing undifferentiated pluripotent stem cells

A stem cell, undifferentiated technology, applied in the field of preparing differentiated cells, undifferentiated pluripotent stem cells, and removing undifferentiated pluripotent stem cells, can solve the problems of PSCs without research and investigation drugs

Inactive Publication Date: 2020-04-21
ACAD SINIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Although cardiac glycosides can act as multiple signaling sensors, no studies have investigated whether these drugs can eliminate undifferentiated PSCs while sparing their progeny or differentiated cells

Method used

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  • Method for removing undifferentiated pluripotent stem cells
  • Method for removing undifferentiated pluripotent stem cells
  • Method for removing undifferentiated pluripotent stem cells

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Embodiment

[0123] An important safety consideration for the use of human pluripotent stem cells (hPSCs) is the risk of tumorigenesis, as these cells can form teratomas at ectopic sites after in vivo injection. Thousands of undifferentiated hPSCs are sufficient to induce teratomas in mouse models. Therefore, it is critical to remove all residual undifferentiated hPSCs with teratoma potential prior to the clinical use of hPSC-derived cells. In the present study, the inventors' data demonstrate the cytotoxic effects of cardiac glycosides, such as digitonin, lanatoside C, bufalin, proscalin, in undifferentiated human embryonic stem cells (hESCs). Original A, digitoxin, digitoxin, and ouabain. This phenomenon was not observed in human bone marrow mesenchymal stem cells (hBMMSCs). Most importantly, digitonin and lanatoside C did not affect the differentiation ability of stem cells. Consistently, the viability of hESC-derived MSCs, neurons, endothelial cells, and endoderm of hepatocytes was ...

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Abstract

The present invention relates to a method for removing undifferentiated embryonic stem cells. In particular, the present invention relates to a method for removing undifferentiated embryonic stem cells by using cardiac glycosides (CGs). The present invention also relates to a method for preparing differentiated cells in which undifferentiated embryonic stem cells have been removed and a method forcell therapy by using such differentiated cells.

Description

[0001] related application [0002] This application is based on 35 U.S.C. §119, claiming the benefit of U.S. Provisional Patent Application No. 62 / 516,437, filed June 7, 2017, the disclosure of which is hereby incorporated into this application by reference. technical field [0003] The present invention relates to a method for removing undifferentiated pluripotent stem cells. Specifically, the present invention relates to a method for removing oncogenic undifferentiated pluripotent stem cells using cardiac glycosides (CGs). The present invention also relates to a method of producing differentiated cells in which undifferentiated pluripotent stem cells are removed, and to a method of using such differentiated cells for cell therapy. Background technique [0004] Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are human pluripotent stem cells with unique properties of self-renewal (the ability to replicate almost infinitely) and pluripotency (t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61K31/704
CPCA61K31/585A61K31/7048C12N5/0081C12N5/0606A61P35/00C07D307/04C12N5/0668
Inventor 吕仁林禹岑
Owner ACAD SINIC