Fibronectin stabilizer and fibronectin preparation added with corresponding stabilizer

A technology of fibronectin and stabilizer, which is applied in the direction of animal/human protein, specific peptides, chemical instruments and methods, etc. It can solve the problems of weakening the repair effect, affecting the uniformity of coating, and easily clogging nozzles due to flocculent precipitation

Active Publication Date: 2022-02-11
ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fibronectin after coagulation is not easy to disperse again, which reduces the actual effective dose in the system
When applied in the field of beauty and skin care, the existence of protein precipitation will affect the uniformity of coating and weaken its repairing effect
In addition, if the fibronectin aqueous solution is used as a spray, the flocculent precipitate will easily block the nozzle, which will directly cause the product to be unusable

Method used

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  • Fibronectin stabilizer and fibronectin preparation added with corresponding stabilizer
  • Fibronectin stabilizer and fibronectin preparation added with corresponding stabilizer
  • Fibronectin stabilizer and fibronectin preparation added with corresponding stabilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Single-component stabilizer screening experiment

[0044] In this experiment, sodium alginate, collagen hydrolyzate, carrageenan, soybean polysaccharide, dextran, polyethylene glycol, glycerin, konjac glucomannan and other substances that may be used as stabilizers were used as the research objects, and the coagulation was accelerated by high temperature. Experimentally evaluate the influence of these substances on the aggregation stability of fibronectin aqueous solution, in order to screen out the stabilizers with good stabilizing effect.

[0045] The experimental process is:

[0046] (1) Preparation of fibronectin aqueous solution: prepare fibronectin stock solution according to the method recorded in patent document CN105950576, then concentrate the prepared fibronectin stock solution or add deionized water to adjust the concentration of fibronectin aqueous solution to 0.5mg / mL,- Store at 20°C for later use.

[0047] (2) Prepare different substance s...

Embodiment 2

[0057] Example 2: Two-component compound preparation of sodium alginate and collagen hydrolyzate to prepare fibronectin stabilizer

[0058] (1) Preparation of fibronectin stabilizer:

[0059] According to the dosages of sodium alginate and collagen hydrolyzate in Example 2-1 to Example 2-12 in Table 2, fully dissolve 1-5g of sodium alginate and 1-5g of collagen hydrolyzate in water to prepare a 200mL aqueous solution to obtain Fibronectin stabilizer required for each example.

[0060] (2) Preparation of fibronectin preparation:

[0061] Dissolve and thaw fibronectin with a concentration of 0.5-5.0 mg / mL and stored at -20°C, take 40 mL; take 10 mL of the fibronectin stabilizer prepared in step (1) corresponding to each example in Table 2, and stir slowly While adding the thawed fibronectin aqueous solution, stirring was continued for 10 minutes after the addition was completed, sterilized and sealed for preservation.

[0062] (3) Measure the ΔAbs of the fibronectin preparati...

Embodiment 3

[0066] Example 3: Sodium alginate, collagen hydrolyzate and glycerin three-component composite preparation of fibronectin stabilizer

[0067] (1) Preparation of fibronectin stabilizer:

[0068] According to the dosages of sodium alginate, collagen hydrolyzate and glycerin in Table 3, Example 3-1 to Example 3-25, respectively disperse 1 to 5 g of sodium alginate, 1 to 5 g of collagen hydrolyzate and 10 to 50 g of glycerin in water Prepare 200mL aqueous solution to obtain the fibronectin stabilizer required by each embodiment.

[0069] (2) Preparation of fibronectin preparation:

[0070] Take 10 mL of the fibronectin stabilizer prepared in step (1) corresponding to each embodiment in Table 2, add the thawed fibronectin aqueous solution while stirring slowly, continue stirring for 10 minutes after the addition is completed, sterilize, and seal and store.

[0071] (3) Measure the ΔAbs of the fibronectin preparation prepared in step (2) according to the operation method of the abov...

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Abstract

The invention belongs to the application field of macromolecular proteins, and specifically discloses a fibronectin stabilizer and a fibronectin preparation added with the corresponding stabilizer. The active ingredient of the fibronectin stabilizer is sodium alginate or a mixture of sodium alginate and collagen hydrolyzate or a mixture of sodium alginate, collagen hydrolyzate and glycerin. The fibronectin preparation contains fibronectin and the fibronectin stabilizer. All the fibronectin stabilizers provided by the invention can improve the aggregation stability and dispersion stability of the fibronectin aqueous solution, and are beneficial to maintain the stability of the fibronectin aqueous solution in a long period of time. The fibronectin preparation prepared by the invention has excellent aggregation stability and dispersion stability, and can be stored for a long time at room temperature; moreover, it has a wide range of applications and has important popularization and application value.

Description

technical field [0001] The invention belongs to the application field of macromolecular proteins, and specifically relates to some fibronectin aqueous solution system stabilizers, and also relates to fibronectin preparations added with corresponding stabilizers. Background technique [0002] Fibronectin (also known as fibronectin, FN for English abbreviation) is a macromolecular condensed glycoprotein with a molecular weight of 470-500kDa, which is widely present in animal tissues and interstitial fluids, such as plasma, milk, amniotic fluid and saliva, etc. . Fibronectin is a growth factor with important biological functions in animals, and has a variety of biological properties due to its multiple functional domains in its structure. At present, fibronectin has been widely used in the fields of cell culture, nerve repair and clinical infusion. A wound dressing made from modified porcine plasma fibronectin has also obtained an invention patent. Fibronectin also has great...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78
CPCC07K14/78
Inventor 刘捷刘畅汤克勇石懿瑾范莹莹翟婧杙孙嘉悦宋玮于博晗
Owner ZHENGZHOU UNIV
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