A method for hatching fertilized eggs of seawater medaka

A fertilized egg and seawater technology, applied in climate change adaptation, application, fish farming, etc., can solve problems such as thickening and inevitable blackening of fertilized eggs

Active Publication Date: 2021-08-31
山东省海洋科学研究院青岛国家海洋科学研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two hatching methods can effectively save water and obtain larvae, but no matter which method, it is inevitable that the fertilized eggs will turn black, thicken, and die in the later stage of hatching

Method used

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  • A method for hatching fertilized eggs of seawater medaka
  • A method for hatching fertilized eggs of seawater medaka
  • A method for hatching fertilized eggs of seawater medaka

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A kind of efficient seawater medaka (Oryzias melastigma) fertilized egg incubation method is characterized in that:

[0026] The physicochemical conditions of described incubation method are as follows:

[0027] The experimental seawater is taken from non-polluted seawater in the field. Before use, it needs to be filtered by sediment sand and absorbent cotton. The salinity is 30±1‰; the hatching temperature is 26-28°C; the air humidity is about 40-60%; Large, 80-125μmol·m -2 ·s -1 It is more conducive to the hatching of fertilized eggs; the light cycle is 14:10, and the lighting period is from 6 am to 8 pm.

[0028] The hatching process of the fertilized eggs diluted in the salinity gradient is as follows:

[0029] Step 1. Place the broodstock with eggs in a petri dish covered with absorbent cotton, carefully suck the fertilized eggs with a plastic straw, and move them to the dissecting microscope for observation. Select the normally fertilized zygotes and transfer ...

Embodiment 2

[0036] The fertilized eggs of medaka hatched in pure seawater were used as the control group. The physical and chemical conditions for hatching were exactly the same as in Example 1. Natural seawater filtered by absorbent cotton was used throughout the hatching process, with a salinity of 30±1‰. In the later stage of hatching, a large number of fertilized eggs were adhered by microalgae ( figure 2 ), the egg shell of the fertilized egg thickens and turns black, and the surface filaments will adhere to residual bait, feces and other particulate matter in the water body ( image 3 ), causing some larvae to emerge from the membrane, only the tail trembled out, the head was stuck in the membrane, and finally died due to hypoxia ( Figure 5 ). The hatching rate of the pure seawater control group was only about 67%, significantly lower than that of Example 1. At the same time, compared with the almost simultaneous hatching of larvae in Example 1, the hatching period of fertilized...

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Abstract

The invention relates to a method for hatching fertilized eggs of seawater medaka, which belongs to the field of aquaculture. The method of the invention hatches the fertilized eggs in seawater with different salinity gradients, and the physical and chemical conditions for hatching are temperature 26-28°C and humidity 40- 60%, light intensity 80‑125μmol m ‑2 ·s ‑1 , the light-to-dark ratio is 14:10; the method of the present invention can obtain fertilized eggs with high cleanliness, which is convenient for experimental operations such as embryological experimental observation and microinjection, and the hatching rate of fertilized eggs can be as high as 94.7%. And the hatched seawater medaka larvae can develop, grow, and reproduce normally after long-term follow-up observation, and no abnormal phenomena have been found.

Description

technical field [0001] The invention relates to the field of fish breeding, in particular to a method for efficiently hatching fertilized eggs of marine model organism medaka. Background technique [0002] Seawater medaka (Oryzias melastigma) belongs to the phylum Chordate, the class Radix, the order Cleptichthyes, the family Oryzias melastigma, and the genus Oryzias. Its individual is small, and the adult body length is only 28±2mm, which is easy to cultivate artificially in the laboratory. The reproductive cycle is short and sexual maturity can be reached at the age of 3 months. The diameter of the fertilized egg is about 0.9±0.05mm, and the overall color is transparent, which is convenient for observation. Due to the above-mentioned biological advantages, seawater medaka has been recommended as an ideal marine model fish species, and is widely used in biological development, ecotoxicology and molecular biology research. [0003] The spawning type of medaka in seawater ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/17
CPCA01K61/17Y02A40/81
Inventor 宋静静于道德刘凯凯盖珊珊迟雯丹
Owner 山东省海洋科学研究院青岛国家海洋科学研究中心
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