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Nucleic acid aptamer functional extraction material for adsorbing phthalate and preparation method and application thereof

A technology of phthalates and nucleic acid aptamers, which is applied in the field of analytical chemistry to achieve high-efficiency enrichment and separation

Active Publication Date: 2020-05-12
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no report on the detection of PAEs using nucleic acid aptamers combined with solid-phase extraction technology.

Method used

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  • Nucleic acid aptamer functional extraction material for adsorbing phthalate and preparation method and application thereof
  • Nucleic acid aptamer functional extraction material for adsorbing phthalate and preparation method and application thereof
  • Nucleic acid aptamer functional extraction material for adsorbing phthalate and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for preparing a nucleic acid aptamer functional extraction material for adsorbing phthalates, the specific steps are: first, centrifuging the 5'-SH modified nucleic acid aptamer at a speed of 8000 rpm for 10 min before use Finally, open it gently, pipette it into 20 mmol / L prepared BB buffer (pH 7.90), dissolve and mix well, place it in a constant temperature water bath at 90 °C for 3 min for annealing treatment, and then place it at room temperature Naturally cool for about 30 min for later use, and store the nucleic acid aptamer solution in a -20°C refrigerator when not in use. Then accurately weigh the crosslinking agent EDMA whose mass accounts for 15% of the entire prepolymerization solution mass, the organic polymeric functional monomer AMPS whose mass accounts for 2.7% of the entire prepolymerization solution mass, and the binary pore-forming compound whose mass accounts for 81.74% of the entire prepolymerization solution mass. DMF and PEG-10000 (the mas...

Embodiment 2

[0031] Accurately weigh the cross-linking agent EDMA whose quality accounts for 10% of the whole prepolymerization solution quality, and other steps are as in Example 1, and material C can be obtained 1 .

Embodiment 3

[0033] Accurately weigh the cross-linking agent EDMA whose quality accounts for 20% of the whole prepolymerization solution quality, other steps are as embodiment 1, and material C can be obtained 3 .

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Abstract

The invention discloses a nucleic acid aptamer functional extraction material for adsorbing phthalate and a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing a cross-linking agent, a high-hydrophilicity organic polymerization monomer, a binary pore-foaming agent and a PAEs aptamer through a sulfydryl-alkene click chemical reaction toprepare a prepolymer solution, and directly forming the extraction material through a one-step thermal polymerization method. The prepared extraction material is uniform in skeleton structure; the reaction efficiency of the nucleic acid aptamer is high; specific recognizability is good; the extraction material can be well used for high-specificity detection and analysis of PAEs in an actual drinking water sample, can detect phthalate substances with the concentration of 0.38 ng / mL-2. 01 ng / mL in mineral water, and provides a new way for specific recognition and separation of the phthalate substances.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and in particular relates to a nucleic acid aptamer functional extraction material for absorbing phthalates, a preparation method and application thereof. Background technique [0002] Phthalic acid esters (PAEs) have become the most common chemical pollutants in the environment, widely present in water, food, soil, sludge and air. Large-scale use and permanent discharge cause them to easily leach from the environment into the human body, causing endocrine system disorders, affecting the development of genitals, and even causing adverse reactions on the liver, kidneys and testes. Therefore, it is necessary and urgent to establish a simple, accurate and highly sensitive detection method. [0003] Currently the most common method for the detection of PAEs is based on chromatographic techniques. However, it is difficult to directly analyze PAEs due to the relatively low concentration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/285C02F1/28B01J20/30C02F101/34
CPCB01J20/285B01J20/26C02F1/285C02F2101/34
Inventor 吕海霞朱熠门刘志
Owner FUZHOU UNIV
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