Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for separating multiple groups of isomers and detecting 29 kinds of steroid hormones

A technology for steroid hormones and isomers, applied in material separation, measurement devices, analytical materials, etc., can solve difficult problems, achieve high precision, achieve specific separation and high-sensitivity detection, and reduce costs. Effect

Active Publication Date: 2022-04-08
北京金域医学检验实验室有限公司
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is extremely difficult to realize the simultaneous detection of 29 steroid hormones including the entire steroid hormone metabolic pathway

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for separating multiple groups of isomers and detecting 29 kinds of steroid hormones
  • A method for separating multiple groups of isomers and detecting 29 kinds of steroid hormones
  • A method for separating multiple groups of isomers and detecting 29 kinds of steroid hormones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Example 1 provides a liquid chromatography mass spectrometry detection method for sterol hormones, liquid conditions: WatersUPLC BEH C8 1.7um2.1mm × 10mm, the aqueous phase is 0.5mM ammonium fluoride aqueous solution, and the organic phase is pure methanol . The program of the gradient system is: 0-0.5 minutes, the water phase is 60%; the 4th minute, the water phase is reduced to 45%; the 7.5 minute, the water phase is reduced to 25%; the 8.2 minute, the water phase is reduced to 10%; At 8.3 minutes, the water phase is 2%, and at 9-10 minutes, the water phase is 60%. Mass spectrometry conditions: ion source temperature 600°C, desolvated gas 1100L / Hr, using multiple reaction monitoring mode.

[0048] In Example 1, the ion source temperature of the liquid chromatography tandem mass spectrometer in step 02 was set to 600°C.

[0049] In Example 1, the desolvation gas of the liquid chromatography tandem mass spectrometer in step 02 is adjusted according to the t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention proposes a method for separating multiple groups of isomers and detecting 29 kinds of steroid hormones. By optimizing the mobile phase and elution conditions of ultra-high performance liquid chromatography, multiple groups of isomers can be separated , by optimizing the liquid phase conditions and mass spectrometry parameters, different steroid hormones with a content span of ng to pg levels can be detected simultaneously, and the simultaneous detection of the content of 29 steroid hormones including multiple groups of isomers in the sample is realized.

Description

technical field [0001] The invention relates to the technical field of steroid hormone detection, in particular to a method for separating multiple groups of isomers and detecting 29 steroid hormones. Background technique [0002] Steroid hormones mainly include adrenocortical hormones and sex hormones. Among them, adrenal cortex hormones include mineralocorticoids and glucocorticoids, and sex hormones include progesterone, androgen, and estrogen. [0003] Steroid hormones are obtained from cholesterol through a series of enzyme catalysis, which are all cyclopentane polyhydrophenanthrene derivatives in structure, with many structural similarities and even isomers (such as 17-hydroxyprogesterone and 11- Deoxycorticosterone, 11-deoxycortisol, 21-deoxycortisol and corticosterone, testosterone and epitestosterone, androsterone and epiandrosterone, etc.), and the hormone content in the human body is widely distributed in ng-pg level, estrogen and Aldosterone and other contents ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 孔许净胡滨陈宝荣樊晓敏姚珺孙慧颖侯生根彭宝生
Owner 北京金域医学检验实验室有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com