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POSS (Polyhedral Oligomeric Silsesquioxane) organic-inorganic hybrid molecular imprinted monolithic column capable of specifically recognizing ochratoxin A and preparation method of monolithic column

A technology of ochratoxin and molecular imprinting, applied in the field of analytical chemistry, can solve the problems that the advantages are not fully demonstrated, and achieve the effect of high-efficiency enrichment and separation

Inactive Publication Date: 2019-02-15
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently there are few reports based on POSS-imprinted monolithic columns, and its advantages have not been fully demonstrated.
In particular, there are no reports on vinyl POSS hybrid monolithic columns combined with molecular imprinting technology and one-pot preparation of molecularly imprinted monolithic columns that can specifically recognize ochratoxin A (OTA)

Method used

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  • POSS (Polyhedral Oligomeric Silsesquioxane) organic-inorganic hybrid molecular imprinted monolithic column capable of specifically recognizing ochratoxin A and preparation method of monolithic column
  • POSS (Polyhedral Oligomeric Silsesquioxane) organic-inorganic hybrid molecular imprinted monolithic column capable of specifically recognizing ochratoxin A and preparation method of monolithic column
  • POSS (Polyhedral Oligomeric Silsesquioxane) organic-inorganic hybrid molecular imprinted monolithic column capable of specifically recognizing ochratoxin A and preparation method of monolithic column

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A POSS organic-inorganic hybrid molecularly imprinted monolithic column that specifically recognizes ochratoxin A and a preparation method thereof, the specific steps are:

[0032] (1) Quartz capillary column pretreatment: wash the fused silica capillary with 0.1 mol / L HCl for 30 minutes, distilled water for 30 minutes, 0.1 mol / L NaOH for 3 hours, distilled water and methanol for 30 minutes, and then Dry at room temperature with 0.4 MPa nitrogen flow for 30 min; in order to introduce C=C bonds into the inner surface of the capillary to bond the hybrid matrix, a 50% volume percent γ-MAPS methanol (v / v) solution was injected into the capillary, Both ends were sealed with silicone rubber, and then the capillary was heated in a water bath at 60 °C for 24 h; after that, the capillary was rinsed with methanol for 30 min, and then dried at 70 °C for 30 min by 0.4 MPa nitrogen for further use;

[0033] (2) According to the mass ratio of 12.7 : 3.8 : 83.5, accurately weigh 2-acr...

Embodiment 2

[0037] A template with an ochratoxin A concentration of 250 ppb was added, and the other steps were as in Example 1 to obtain column A.

Embodiment 3

[0039] A template with a concentration of ochratoxin A of 750 ppb was added, and other steps were as in Example 1 to obtain column C.

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Abstract

The invention discloses a POSS (Polyhedral Oligomeric Silsesquioxane) organic-inorganic hybrid molecular imprinted monolithic column capable of specifically recognizing ochratoxin A and a preparationmethod of the monolithic column and belongs to the field of analytical chemistry. The monolithic column is prepared by the following steps: taking a highly hydrophilic organic polymerization monomer,a cross-linking agent, a template, an initiator and a binary pore-forming agent as raw materials, dissolving to form a uniform solution, injecting the solution into a column to carry out a free radical thermal-initiation polymerization reaction to directly form a monolithic column, and washing the monolithic column, thereby obtaining the finished product. The POSS organic-inorganic hybrid molecular imprinted monolithic column capable of specifically recognizing ochratoxin A prepared in the invention is high in capacity, high in specificity and stable in mechanical property, and the preparationmethod is simple and rapid and is applicable to specific recognition and separation of the ochratoxin A. The detection recovery rate of the molecular imprinted monolithic column on the ochratoxin A in a beer substrate reaches 96.6%, and a novel pathway is provided for specific recognition and separation of the ochratoxin A.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a POSS organic-inorganic hybrid molecularly imprinted integral column for specifically recognizing ochratoxin A and a preparation method thereof. Background technique [0002] Molecular Imprinted Monolithic Column (MIPMC) is a new type of chromatographic packing monolithic column that has developed rapidly in recent years. The chromatographic filler macroporous polymer medium is synthesized by in-situ polymerization method, that is, the solution of monomer, initiator, crosslinking agent and porogen is polymerized in an empty column. Molecularly imprinted monolithic column has the advantages of simple preparation process, fast mass transfer rate, good repeatability, low column pressure, etc. It is a kind of chromatographic stationary phase with great application potential, so it has received more and more attention. Molecularly imprinted monolithic columns are divi...

Claims

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Application Information

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IPC IPC(8): G01N30/60
CPCG01N30/60
Inventor 吕海霞孙浩冉王俊
Owner FUZHOU UNIVERSITY
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