A fluorescent biosensor based on high-gradient magnetic separation and quantum dots and its application

Abstract

The invention provides a fluorescent biosensor based on high-gradient magnetic separation and a quantum point as well as application thereof. The fluorescent biosensor comprises a high-gradient magnetic separation device and a fluorescence detection system; the high-gradient magnetic separation device comprises an annular magnet array, a magnet bracket, a ring channel and an iron bead chain; the annular magnet array and the magnet bracket form an annular magnetic field generator; every two adjacent magnets are mutually repulsed and assembled; two capillary tubes are coaxially fixed and then are nested in the concentric circle of the annular magnet array; the inner capillary tube is filled with the iron bead chain made of a soft magnet material; the iron bead chain can be magnetized by thering magnetic field, so that nanometer magnetic beads are distributed in the ring channel uniformly and are used for capturing a target object. The fluorescent biosensor provided by the invention canbe used for quantitatively detecting microorganisms, is simple to operate, high in separation efficiency and high in sensitivity, and can realize quantitative detection on a small amount of target bacteria in food samples within 2 hours.

Description

technical field [0001] The invention relates to the technical field of biological detection, more specifically, to a microorganism detection method based on high-gradient magnetic separation and quantum dots. Background technique [0002] In recent years, the problem of food safety has been widely concerned by the international community. In my country, pathogenic microorganisms are the most important factor causing food poisoning, which has seriously threatened people's health and caused huge economic losses. A key to preventing and controlling foodborne disease outbreaks is the rapid screening of food for microbial contamination. Existing bacterial detection methods mainly include culture method (for bacteria) or isolation method (for viruses), immunological methods (such as ELISA), and molecular biology methods (such as PCR). The traditional culture or isolation method is the gold standard method, which has high accuracy and sensitivity, but takes a long time; ELISA has ...

AI Technical Summary

Problems solved by technology

The traditional filtration and centrifugation methods are based on the size and mass of the target object for separation without specificity. However, the immunomagnetic separation method developed in recent years is based on the immune reaction of antigen and antibody, using immunomagnetic beads and an external magnetic field to separate the target object from Separation and enrichment from the sample background, although specific, this method can only handle small volume samples, and is basically manual operation, which requires high requirements for operators

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