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Preparation method for medical molecularly-imprinted polymer (MIP) microsphere applied to clinical immunoassay

A technology of molecular imprinting and immune analysis, applied in the field of medical research, can solve the problems of unfavorable large-scale promotion and application, scarcity and expensive raw materials, and difficulty in popularization, etc., and achieve the effect of uniform size, simple preparation method, and small diameter

Inactive Publication Date: 2017-10-03
HUANGHE S & T COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using this method to prepare molecularly imprinted polymer microspheres, the raw materials used are relatively scarce and expensive, and the cost is too high, which is not conducive to large-scale promotion and application, and is difficult to popularize

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for preparing molecularly imprinted polymer microspheres for medical clinical immunoassays, using chloromethyl polystyrene spheres as carriers, modifying the iniferter-terminating agent sodium diethyldithiocarbamate on its surface, and triclosan as template molecule, 4-vinylpyridine as functional monomer, ethylene glycol dimethacrylate as crosslinking agent, and triclosan molecularly imprinted polymer microspheres were prepared by photoinitiated polymerization;

[0037] Specifically include the following steps:

[0038] S1. Polystyrene spheres modified by sodium diethyldithiocarbamate, an iniferter:

[0039] The chloromethyl polystyrene ball carrier is ultrasonically dispersed in ethanol, and the ethanol solution of sodium diethyldithiocarbamate is slowly added dropwise. After the reaction, the resulting product is cleaned and dried in vacuum to obtain a surface graft modified polystyrene ball ;

[0040] S2. Preparation of triclosan molecularly imprinted poly...

Embodiment 2

[0058] A method for preparing molecularly imprinted polymer microspheres for medical clinical immunoassay, the method for preparing 4-chloromethylphenyltrichlorosilane in step S3 is: dissolving 4-chloromethylphenyltrichlorosilane in In anhydrous dimethylformamide, add excess NaH, stir magnetically for 0.5-1.5h, then filter to remove excess NaH; add γ-propyltrimethoxysilane dropwise under magnetic stirring, react at 80°C for 10-14h ; After the reaction is over, wash the precipitate and dry it in vacuum for 10-14h.

[0059] Two functional monomers are used to cooperate with the template molecules to greatly increase the adsorption amount of the molecularly imprinted polymer on the surface of the polymer microspheres and the specific recognition function of the template molecules. At least one of the functional monomers includes hydrophilic and hydrophobic structures, so that the prepared polymer microspheres can be applied to the water phase treatment process, and have larger ad...

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Abstract

The invention relates to the field of medical research, especially to a preparation method for a medical MIP microsphere applied to clinical immunoassay. The method is simple and easily practicable and prepares the MIP microsphere with hydrophilic polymer brushes on its surface through a coupling reaction of a hydrophilic macromolecular chain transfer agent and a MIP microsphere with C-C double bonds on its surface; and the hydrophilic macromolecular chain transfer agent firstly undergoes a chain transfer reaction with free radicals produced by a common radical initiator so as to produce hydrophilic macromolecule free radicals, and then the hydrophilic macromolecule free radicals react with the C-C double bonds on the MIP microsphere so as to obtain the MIP microsphere with the hydrophilic polymer brushes on its surface. The surface of the magnetic MIP microsphere has a great number of hydroxyl groups, so the MIP microsphere is applicable to water-phase treatment, and has great adsorption capacity and selectivity; and the MIP microsphere has good application prospects in fields like biological medicine and clinical medicine.

Description

technical field [0001] The invention relates to the field of medical research, in particular to a method for preparing molecularly imprinted polymer microspheres for medical clinical immunoassay. Background technique [0002] With the rapid development of biology, medicine, chemical industry and other industries, the requirements for the purity of raw materials and products are getting higher and higher, so it is very necessary to separate and purify substances. However, the existing separation methods are complicated in process and high in cost. Molecular imprinting technology provides a new and effective way for this. A new type of separation and purification technology developed on the basis of actual needs. The molecularly imprinted polymer prepared based on this technology has strong resistance to harsh environments, good physical and chemical stability, and long service life. Its unique three-dimensional structure can Selective identification and separation of substan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/30B01J20/26B01J20/28C08F257/02C08F226/06C08F222/14C08F220/06C08F2/48
CPCB01J20/265B01J20/268B01J20/28019B01J20/28057B01J2220/445C08F2/48C08F257/02C08F222/102
Inventor 张保丰蒋爱云姜爱菊
Owner HUANGHE S & T COLLEGE
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