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Preparation and application of CAR-T cells for accurate killing of tumors

A tumor and immune cell technology, applied in the field of CAR-T cell preparation

Pending Publication Date: 2020-05-15
EAST CHINA NORMAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although CAR-T therapy is effective, there are still many problems that need to be solved urgently. One of the major challenges is the homing of CAR-T cells to tumor tissues

Method used

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  • Preparation and application of CAR-T cells for accurate killing of tumors
  • Preparation and application of CAR-T cells for accurate killing of tumors
  • Preparation and application of CAR-T cells for accurate killing of tumors

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Embodiment Construction

[0140] After extensive and in-depth research, the inventors unexpectedly discovered for the first time that engineered immune cells containing chimeric antigen receptor CAR and CXCR4 targeting MUC1 can selectively kill tumor cells, such as tumor cells with high expression of CXCL12 or MUC1, and The higher the expression level, the more significant the killing effect of the CXCR4 engineered CAR-T cells (that is, the engineered immune cells of the present invention). On this basis, the inventors have completed the present invention.

[0141] Glossary

[0142] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0143] As used herein, the term "about" when used in reference to a specifically recited value means that the value may vary by no more than 1% from the recited value. For example, as used herein, the expression "about 100" includes all...

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PUM

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Abstract

The invention provides preparation and application of CAR-T cells for accurate killing of tumors, and specifically provides an engineered immune cell. The engineered immune cell can be used for expressing chimeric antigen receptors CAR and CXCR4 which target MUC1, the engineered immune cell can be used for selectively killing CXCL12 or MUC1-highly expressed tumor cells and other tumor cells, and the CAR-T cells have a significant killing effect on the tumor cells.

Description

technical field [0001] The invention belongs to the field of biotechnology. Specifically, the present invention relates to the preparation and use of CAR-T cells for precise tumor killing. Background technique [0002] Chimeric antigen receptor gene-modified T (chimeric antigen receptor gene-modified T, CAR-T) cell therapy is the current research focus and focus of tumor immune cell therapy. It organically combines the high affinity of antibodies to tumor antigens and the killing mechanism of T lymphocytes, so that CAR-T can specifically recognize tumor cells and directly kill tumor cells without relying on the restriction of MHC. Studies have shown that it has made important progress in the treatment of hematological tumors, and is gradually being extended to the clinical treatment of solid tumors, which is expected to overcome the difficulties in the treatment of solid tumors. A chimeric antigen receptor (CAR) is a fusion molecule consisting of an extracellular antigen-b...

Claims

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Application Information

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IPC IPC(8): C12N5/10C12N15/62C12N15/867C07K19/00A61K39/00A61P35/00
CPCC07K14/7051C07K16/3092C07K14/7158C12N15/86A61K39/001121A61P35/00C07K2319/02C07K2319/03C07K2319/33C12N2740/15043
Inventor 江文正阮丹阳张利周滢张红梅刘明耀席在喜
Owner EAST CHINA NORMAL UNIV