Application of coyne in the preparation of antiallergic drugs

A technology for allergic reactions and drugs, which is applied in the application field of caine in the preparation of anti-allergic drugs, and can solve problems such as adverse reactions and no reports on the application of anti-allergic drugs.

Active Publication Date: 2021-04-20
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current drugs for such diseases all act in the symptom control stage downstream of the allergic reaction mechanism, and the sensitization mediators released by mast cells still exist in the body and have potential toxicity
Long-term use of the above drugs can cause a variety of adverse reactions
At present, there is no report on the application of Coin as an antiallergic drug

Method used

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  • Application of coyne in the preparation of antiallergic drugs
  • Application of coyne in the preparation of antiallergic drugs
  • Application of coyne in the preparation of antiallergic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Coyne inhibits the release of KU812 cell β-hexosaminidase induced by SP

[0026] 1. Experimental materials

[0027] SP (purchased from MCE Company), KU812 cells, RPMI1640 medium (containing penicillin 100U / mL, streptomycin 0.1mg / mL, 20% FBS), Tyrode's solution, Coyne (purchased from Chengdu Pufeide Company), 0.1M Na 2 CO 3 , 0.1M NaHCO 3 , β-hexosaminidase, 0.1M citric acid, 0.1M sodium citrate.

[0028] The used SP (Substance P) refers to the neuropeptide discovered by Von Euler and Gaddum in 1931, the sequence is Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, and the molecular weight is 1340 .

[0029] Further, the purity of SP is ≥97%.

[0030] 2. Experimental method

[0031] KU812 cells were seeded in 96-well plates (1×105 cells / well), and the culture medium was discarded by centrifugation. Coyne solutions (concentrations of 0 μM, 25 μM, 50 μM, 100 μM and 200 μM) were prepared with sterile Tyrode’s solution, added to corresponding well plates, an...

Embodiment 2

[0035] Example 2 Coin inhibits mouse toe swelling and exudation caused by SP

[0036] 1. Experimental materials

[0037] SP (purchased from MCE Company), KU812 cells, RPMI1640 medium (containing penicillin 100U / mL, streptomycin 0.1mg / mL, 20% FBS), Tyrode's solution, Coyne (purchased from Chengdu Pufeide Company), Human TNF-αELISA kit (purchased from Beijing Yiqiao Shenzhou Company)

[0038] The used SP (Substance P) refers to the neuropeptide discovered by Von Euler and Gaddum in 1931, the sequence is Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2, and the molecular weight is 1340 .

[0039] Further, the purity of SP is ≥97%.

[0040] 2. Experimental method

[0041] KU812 cells were seeded in 96-well plates (1×105 cells / well), and the culture medium was discarded by centrifugation. Coyne solutions (concentrations of 0 μM, 25 μM, 50 μM, 100 μM and 200 μM) were prepared with sterile Tyrode’s solution, added to corresponding well plates, and a blank control group was set. ...

Embodiment 3

[0045] Example 3 Coyne inhibits mouse toe swelling, exudation and toe HE staining caused by SP

[0046] 1. Experimental materials

[0047] SP (purchased from MCE Company), 6-8 week-old male C57 mice (purchased from the Experimental Animal Center of Xi'an Jiaotong University), coyne (purchased from Chengdu Pufeide), 0.4% Evans blue solution, 3.5% chlorine hydrate Aldehyde solution, PBS solution, saline, acetone.

[0048] 2. Experimental method

[0049] The mice were divided into blank control group, positive control group and coine administration groups of various concentrations. Coyne solutions of different concentrations (3.7mg / kg, 7.5mg / kg, 15mg / kg respectively) were prepared with physiological saline for intraperitoneal injection. After 30 minutes, mice were anesthetized with 3.5% chloral hydrate solution, and 0.4% Evans blue solution was injected into the tail vein. Measure the thickness of the left and right paws of the mice in each group, then subcutaneously inject 5...

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PUM

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Abstract

The invention discloses the application of coyne in the preparation of anti-allergic drugs, by comparing the degranulation of KU812 cells, the degree of swelling of the toes, the degree of Evans blue exudation of the toes, HE staining of foot skin tissue sections, and the release of serum histamine To evaluate the drug group to be tested, the normal saline control group and the normal control group, to evaluate the therapeutic effect of the drug on allergic diseases, and to determine that coyne can be used in the preparation of anti-allergic drugs. Coin can effectively alleviate the symptoms of local and systemic allergic reactions in mice; reduce mast cell degranulation and significantly improve the symptoms caused by allergic reactions.

Description

technical field [0001] The invention belongs to the technical field of preparation of antiallergic drugs, and relates to the application of coyne in the preparation of antiallergic drugs. Background technique [0002] Coin (5,7-dihydroxyflavone), also known as chrysin, is a main flavonoid in the extracts of poplar propolis and many plants (such as Bignoniaceae plant wood butterfly), which has anti-inflammatory, anti-tumor , anti-allergic, anti-oxidation, anti-aging, anti-diabetes and immune regulation and other biological activities, such as anti-oxidation, anti-virus, hypoglycemic, anti-anxiety, etc., has protection for various organs (such as liver, kidney, colon) effect. Extensively present in honey and propolis. [0003] Anaphylaxis is not an immune response, and its etiology and mechanism are completely different from anaphylaxis. In immunology, the adverse reaction caused by the combination of antigen (or hapten) into the sensitized body and its specific antibody ag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61P37/08
CPCA61K31/352A61P37/08
Inventor 王楠吴媛媛张永竟王珏
Owner XI AN JIAOTONG UNIV
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