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Sealed type adherent stem cell culture factory

A stem cell culture, sealed technology, applied in the direction of tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of inapplicable stem cell culture and expansion, limited wide use, unstable structure, etc. Achieve the effect of flexible use, increased output, and simple operation

Pending Publication Date: 2020-05-29
京诺(上海)细胞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This cell culture device also has the defects of poor sealing and unstable structure, which is not suitable for the cultivation and expansion of stem cells, which limits its wide use

Method used

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  • Sealed type adherent stem cell culture factory
  • Sealed type adherent stem cell culture factory
  • Sealed type adherent stem cell culture factory

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] see figure 1 As shown, this embodiment provides a sealed adherent stem cell culture factory, including a bottom box 10, a cover 30, and at least one box 20 stacked between the bottom box 10 and the cover 30 The whole of the sealed adherent stem cell culture factory is a layered structure composed of at least one box body 20 stacked between the bottom box 10 and the cover body 30, and the bottom box 10, the box body 20 and the cover body are stacked up and down 30 through the sealing mechanism 40 can realize fast buckle connection, the sealing mechanism 40 can adopt bolts or other fixed parts of any structure, to ensure that the bottom box 10, the box body 20 and the cover body 30 through the sealing mechanism 40 have Good sealing.

[0051] In this example, see Figure 4-5 As shown, the two adjacent corners of the box body 20 are respectively provided with an air inlet passage 21 and an air outlet passage 22, and an arc-shaped shroud 23 is vertically arranged along the...

Embodiment 2

[0064] see Figure 6-11 As shown, different from the above-mentioned embodiment 1, this embodiment also provides a sealing mechanism 40 suitable for the box body 20 and the cover body 30 of the sealed adherent stem cell culture factory. Each sealing mechanism 40 on the box body 20 and / or the cover body 30 includes: a sealing column 41 arranged on the inner side wall of the box body 20 and / or the cover body 30, a vertically arranged A T-shaped push rod 42 in the accommodation chamber, and an elastic buckle disposed in the accommodation chamber and below the tapered top 44 .

[0065] In this example, see Image 6 As shown, the sealing column 41 on the box body 20 and / or the cover body 30 is arranged on the inner side wall of the box body 20 and / or the cover body 30, and the sealing column 41 is provided with a hollow The accommodating chamber, and the top and bottom of the sealing column 41 are respectively provided with bayonet openings communicating with the accommodating ch...

Embodiment 3

[0073] Based on the above example, see Figure 2-3 As shown, this embodiment also provides a sealing mechanism 40 suitable for the bottom box 10 of the sealed adherent stem cell culture factory. It should be noted that, in this embodiment, the sealing mechanism 40 of the bottom box 10 is only a sealing column 41 with an accommodating chamber and an upper and lower bayonet. The sealing column 41 on the bottom box 10 and the box above it The sealing mechanism 40 of the body 20 is snap-fit ​​connected.

[0074] In this example, see Figure 2-3 As shown, each of the sealing mechanisms 40 on the bottom box 10 includes: a sealing column 41 arranged on the inner wall of the bottom box 10, and a hollow accommodation chamber is arranged in the sealing column 41, and the The top and the bottom of the sealing column 41 are respectively provided with bayonet sockets communicating with the containing chamber; and a T-shaped ejector rod 42 vertically arranged in the described containing c...

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Abstract

The invention discloses a sealed type adherent stem cell culture factory. The sealed type adherent stem cell culture factory comprises a bottom box, a cover body and at least one box body, wherein anair inlet passage and an air outlet passage are separately formed at two adjacent corners of each box body, arc-shaped shrouding plates are vertically arranged along edges of the air inlet passages and edges of the air outlet passages, and side holes are formed in upsides of the arc-shaped shrouding plates; an air inlet pipe and an air outlet pipe, which penetrate up and down, are separately arranged at two adjacent corners of the cover body; and a plurality of up-and-down-corresponding sealing mechanisms are separately arranged on internal sidewalls of the bottom box, box bodies and cover body, movable inverted-trapezoidal snapping buckles are arranged at bottoms of the sealing mechanisms, snapping ports matched with the inverted-trapezoidal snapping buckles are arranged at tops of the sealing mechanisms, and the bottom box, the box bodies and the cover body, which are adjacently arranged up and down, are connected through the snapping buckles of the sealing mechanisms. The sealed type adherent stem cell culture factory disclosed by the invention is flexible and convenient in use; and the operation is simple, the sealing performance of connection is good, the stability is high, and the yield of a single batch can be effectively increased, so that the sealed type adherent stem cell culture factory is applicable to factory large-scale production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a sealed type adherent stem cell culture factory. Background technique [0002] A cell factory is a cell culture device that saves space by utilizing the maximum culture surface in a limited space; it can be used for industrial scale production such as vaccines, monoclonal antibodies or biopharmaceuticals, especially suitable for adherent cells , can also be used for suspension culture, and does not change the kinetic conditions of cell growth when scaling up from laboratory scale, making scale-up simple and easy, with low risk of contamination and space-saving. [0003] For example, the published patent CN110283725A discloses a new type of multi-layer adherent cell culture container structure, including a multi-layer cell factory body, a bayonet cover and a vent cover. Multi-layer culture surface, the main body of the multi-layer cell factory is equipped with a multi-layer culture ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/04C12M1/04
CPCC12M23/24C12M23/38C12M23/42C12M29/00
Inventor 王晓冰席刚
Owner 京诺(上海)细胞生物技术有限公司