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Combined embryonic stem cell sealed culture device

A technology for embryonic stem cells and culture devices, which is applied in the field of combined embryonic stem cell sealed culture devices, can solve the problems of inapplicable stem cell culture and expansion, limited wide use, unstable structure, etc., and achieves flexible use, increased production, and simple operation Effect

Pending Publication Date: 2020-05-29
上海睿健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This cell culture device also has the defects of poor sealing and unstable structure, which is not suitable for the cultivation and expansion of stem cells, which limits its wide use

Method used

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  • Combined embryonic stem cell sealed culture device
  • Combined embryonic stem cell sealed culture device
  • Combined embryonic stem cell sealed culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] see figure 1 As shown, this embodiment provides a combined embryonic stem cell sealed culture device, including a bottom box 10, a cover 30 and at least one box 20 stacked between the bottom box 10 and the cover 30 ; Wherein: the combined embryonic stem cell sealed culture device is a layered structure composed of at least one box body 20 stacked between the bottom box 10 and the cover body 30, the bottom box 10, the box body 20 and the box body 20 stacked up and down. The fast buckle connection between the lids 30 can be realized through the sealing mechanism 40, and the sealing mechanism 40 can use bolts or other fixed parts of any structure to ensure that the bottom box 10, the box body 20 and the lid body 30 pass through the sealing mechanism 40. There is good sealing between.

[0056] In this example, see Figure 4-8 As shown, the box body plate 21 at a corner of the box body 20 is provided with a communication hole 22, and an arc-shaped coaming plate 23 is verti...

Embodiment 2

[0067] see Figure 9-14 As shown, different from the above-mentioned embodiment 1, this embodiment also provides a kind of each seal on the box body 20 and / or the cover body 30 suitable for the combined embryonic stem cell sealed culture device. mechanism 40, each sealing mechanism 40 on the box body 20 and / or the cover body 30 includes: a sealing post 41 arranged on the inner side wall of the box body 20 and / or the cover body 30, A T-shaped push rod 42 vertically arranged in the accommodating chamber, and an elastic buckle arranged in the accommodating chamber and below the tapered apex 44 .

[0068] In this example, see Figure 9 As shown, the sealing column 41 on the box body 20 and / or the cover body 30 is arranged on the inner side wall of the box body 20 and / or the cover body 30, and the sealing column 41 is provided with a hollow The accommodating chamber, and the top and bottom of the sealing column 41 are respectively provided with bayonet openings communicating with...

Embodiment 3

[0076] Based on the above example, see Figure 2-3 As shown, this embodiment also provides a sealing mechanism 40 suitable for the bottom box 10 of the combined embryonic stem cell sealed culture device. It should be noted that, in this embodiment, the sealing mechanism 40 of the bottom box 10 is only a sealing column 41 with an accommodating chamber and an upper and lower bayonet. The sealing column 41 on the bottom box 10 and the box above it The sealing mechanism 40 of the body 20 is snap-fit ​​connected.

[0077] In this example, see Figure 2-3 As shown, each of the sealing mechanisms 40 on the bottom box 10 includes: a sealing column 41 arranged on the inner wall of the bottom box 10, and a hollow accommodation chamber is arranged in the sealing column 41, and the The top and the bottom of the sealing column 41 are respectively provided with bayonet sockets communicating with the containing chamber; and a T-shaped ejector rod 42 vertically arranged in the described con...

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Abstract

The invention discloses a combined embryonic stem cell sealed culture device. The combined embryonic stem cell sealed culture device comprises a bottom box, a cover body and at least one box body, wherein a communicating hole is arranged on a box body plate at one corner of each box body; an arc-shaped surrounding plate is perpendicularly arranged along the edge of each communicating hole; in thebox bodies stacked from top to bottom, the box bodies at odd positions and the box bodies at even positions are integrally in central symmetry on a horizontal plane; an air inlet pipe and an air outlet pipe which longitudinally penetrate the cover body are respectively arranged at two opposite corners of the cover body; the inner side walls of the bottom box, the box bodies and the cover body arerespectively provided with a plurality of vertically corresponding sealing mechanisms; and the bottom box, the box bodies and the cover body which are vertically adjacent are in buckled connection through the sealing mechanisms. According to the combined embryonic stem cell sealed culture device, a cell culture fluid and oxygen are supplemented to each layer of culture area in a series connectionmanner, so the cell culture fluid can be guaranteed to freely perform gas exchange; meanwhile, the combined embryonic stem cell sealed culture device is flexible and convenient to use, simple to operate, good in connection sealing performance, high in stability and applicable to large-scale production in factories.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a combined sealed culture device for embryonic stem cells. Background technique [0002] A cell factory is a cell culture device that saves space by utilizing the maximum culture surface in a limited space; it can be used for industrial scale production such as vaccines, monoclonal antibodies or biopharmaceuticals, especially suitable for adherent cells , can also be used for suspension culture, and does not change the kinetic conditions of cell growth when scaling up from laboratory scale, making scale-up simple and easy, with low risk of contamination and space-saving. [0003] For example, the published patent CN110283725A discloses a new type of multi-layer adherent cell culture container structure, including a multi-layer cell factory body, a bayonet cover and a vent cover. Multi-layer culture surface, the main body of the multi-layer cell factory is equipped with a multi-layer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00C12M1/04
CPCC12M23/02C12M23/44C12M23/58
Inventor 王晓冰席刚
Owner 上海睿健生物科技有限公司