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Application of natural product patulin in weed control

A technology for patulin and weeds, which is applied in the chemical, application, biocide and other directions for biological control, can solve the problem of no herbicidal activity, etc., and achieves simple structure, easy artificial synthesis, broad-spectrum herbicidal active effect

Inactive Publication Date: 2020-06-05
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on patulin mainly focuses on its bactericidal and insect-resistant functions, its influence on cells and cell components, toxicological mechanism and detection methods, etc. There is no systematic research and report on herbicidal activity, which is also the subject of this patent. the innovation of

Method used

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  • Application of natural product patulin in weed control
  • Application of natural product patulin in weed control
  • Application of natural product patulin in weed control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: The influence of patulin on the photosynthesis of Eupatorium adenophorum

[0033] Dissolve patulin in DMSO and prepare 25 μM, 50 μM, 100 μM, and 200 μM solutions with distilled water; take the leaves of Eupatorium adenophorum, use a puncher to punch the leaves into leaf disks with a diameter of 7 mm, and put them into In the prepared solutions of different concentrations, vacuum filtration for 15min, 400μmol(photos)m -2 the s -1After treatment at 25°C for 3, 6, and 12 hours under light intensity conditions, the chlorophyll fluorescence parameters were measured with a plant efficiency analyzer (HandyPEA). 30 leaf discs were tested for each concentration. The results are shown in Table 1. At the same time, the effect of patulin on the electron transport chain of the photosynthetic system was tested, and the results are shown in Table 2.

[0034] Table 1 Effect of patulin on chlorophyll fluorescence parameters of Eupatorium adenophorum leaves

[0035] ...

Embodiment 2

[0040] Example 2: Broad-spectrum pathogenic effect of patulin

[0041] Take a small amount of patulin, first dissolve it with a small amount of DMSO, and then use deionized water to prepare a solution with a concentration of 500ppm. At the same time, set an equivalent concentration of DMSO solvent control and water control, and take a number of leaves of weeds or crops with the same growth. , rinsed with tap water, then rinsed with sterile water, blotted the water on the leaves, put the back of the leaves up in a petri dish covered with moist filter paper; needled with a dissecting needle at the back of the leaves 1-2cm away from the edge of the leaves Cause slight damage; use a 200 μL pipette to pipette 20 μL solution and drop it on the acupuncture hole, repeat each treatment more than 6 times, measure the lesion area after 48 hours at 25 ° C and natural light, and the pathogenicity grading standard see Table 3, test statistical results are shown in Table 4 and Table 5.

[0...

Embodiment 3

[0051] Example 3: Pathogenic susceptibility to patulin

[0052] A small amount of patulin was prepared with deionized water to prepare solutions with different concentrations of 5ppm, 25ppm, 50ppm, 100ppm, 200ppm, 300ppm, 400ppm, etc., and a corresponding concentration of DMSO control and water control were set. Sow the seeds of the test plants (Table 7, 8) in pots with a diameter of 9 cm, set 4 groups of repetitions, and cultivate them in a greenhouse at a temperature of 28 ° C. When the test plants reach the 3-4 leaf stage, carry out stem and leaf treatment , the dosage is 100mL / m 2 , 7 days after application, the incidence was counted, and the susceptibility of patulin to each tested plant was tested. See Table 6 for the standard of leaf toxicity. The disease index is calculated according to the formula: disease index=∑(level×number of plants)×100 / 4 / number of weed plants per treatment. See Table 7 and Table 8 for the statistical results of diseases.

[0053] Table 6 Stan...

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Abstract

The invention discloses an application of a natural product patulin in weed control. Patulin is used as an active substance to develop a biological herbicide, which can be used for controlling weeds,and can be used for controlling weeds such as crab grass, false daisy, bluegrass, amaranthus retroflexus, microstegium vimineum, crofton weed, eupatorium adenophorum, morning glory, thyrocarpus sampsonii and plantain herb in crop fields (such as rice, corn, wheat and cotton) and non-cultivated areas. Patulin is preferably used for controlling crab grass, amaranthus retroflexus, microstegium vimineum and crofton weed. Patulin has the characteristics of wide weeding spectrum, environment friendliness, rapidness and high efficiency, and the weeding activity is improved by adding the auxiliary agent.

Description

technical field [0001] The invention belongs to the field of agricultural pesticides and relates to the use of natural product patulin for weed control. Background technique [0002] Herbicides are substances that can completely or selectively kill weeds and are used to eliminate or inhibit plant growth. Herbicide is one of the main pesticides used in modern agricultural production, which is of great significance to ensure the safety of food production. However, the long-term and extensive use of chemical herbicides has brought about increasingly serious environmental pollution, weed resistance and public health problems. In recent years, more and more toxic traditional chemical herbicides have been banned in countries all over the world. Safe, environmentally friendly and efficient biological herbicides are getting more and more attention. Biological herbicides refer to preparations for weed control developed using living tissues of plants, animals and microorganisms or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P13/00A01G13/00
CPCA01G13/00A01N43/90
Inventor 陈世国柳伟哲强胜王赫
Owner NANJING AGRICULTURAL UNIVERSITY
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