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Zeolite imidazolate framework-horseradish peroxidase biomineralization embedded precoating and its preparation and application in ultrafiltration membrane water purification

A technology of horseradish peroxidase and zeolite imidazolate, which is applied in the field of water treatment, can solve problems such as the application of immobilized enzyme on zeolite imidazolate framework materials, and achieves mature, stable and reliable, improved diffusivity, and pollution purification. Effects of functional avoidance

Active Publication Date: 2021-08-13
清上(苏州)环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there have been no relevant reports on the application of immobilized enzymes on zeolite-like imidazolate framework materials in ultrafiltration water purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The embodiment of the present invention provides a zeolite imidazolate skeleton-horseradish peroxidase biomineralization embedding pre-coating and a preparation method thereof, specifically as follows:

[0042] S1. Preparation of ZIF-8 / Catalase:

[0043] 1) Prepare a 2-methylimidazole solution with a concentration of 0.16mol / L and a zinc acetate solution with a concentration of 0.04mol / L with pure water, respectively;

[0044] 2) adding hydrochloric acid or sodium hydroxide solution to adjust the pH value of the 2-methylimidazole solution to 10.0;

[0045] 3) Add 4.0mg / mL horseradish peroxidase (HRP) and 5.0mg / mL bovine serum albumin (BSA) into the 2-methylimidazole solution under full stirring until completely mixed ;

[0046] 4) Add zinc acetate solution under sufficient stirring conditions, wherein the molar ratio of zinc acetate to 2-methylimidazole is 1:4;

[0047] 5) The above mixed solution was stirred rapidly for 10 s at a stirring speed of 1000 rpm, and left t...

Embodiment 2

[0058] The embodiment of the present invention provides a zeolite imidazolate skeleton-horseradish peroxidase biomineralization embedding pre-coating and a preparation method thereof, specifically as follows:

[0059] S1. Preparation of ZIF-8 / Catalase:

[0060] 1) Prepare a 2-methylimidazole solution with a concentration of 0.16mol / L and a zinc acetate solution with a concentration of 0.04mol / L with pure water, respectively;

[0061] 2) adding hydrochloric acid or sodium hydroxide solution to adjust the pH value of the 2-methylimidazole solution to 10.0;

[0062] 3) Add 4.0mg / mL horseradish peroxidase (HRP) and 5.0mg / mL bovine serum albumin (BSA) into the 2-methylimidazole solution under full stirring until completely mixed ;

[0063] 4) Add zinc acetate solution under sufficient stirring conditions, wherein the molar ratio of zinc acetate to 2-methylimidazole is 1:2;

[0064] 5) The above mixed solution was stirred rapidly at a stirring speed of 800rpm for 12s, and left to...

Embodiment 3

[0075] The embodiment of the present invention provides a zeolite imidazolate skeleton-horseradish peroxidase biomineralization embedding pre-coating and a preparation method thereof, specifically as follows:

[0076] S1. Preparation of ZIF-8 / Catalase:

[0077] 1) Prepare a 2-methylimidazole solution with a concentration of 0.16mol / L and a zinc acetate solution with a concentration of 0.04mol / L with pure water, respectively;

[0078] 2) adding hydrochloric acid or sodium hydroxide solution to adjust the pH value of the 2-methylimidazole solution to 10.0;

[0079] 3) Add 4.0mg / mL horseradish peroxidase (HRP) and 5.0mg / mL bovine serum albumin (BSA) into the 2-methylimidazole solution under full stirring until completely mixed ;

[0080] 4) Add zinc acetate solution under sufficient stirring conditions, wherein the molar ratio of zinc acetate to 2-methylimidazole is 1:5;

[0081] 5) The above mixed solution was stirred rapidly for 10 s at a stirring speed of 1000 rpm, and left...

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Abstract

The invention discloses a zeolite-like imidazolate skeleton-horseradish peroxidase biomineralization embedded pre-coating and its preparation and application in ultrafiltration membrane water purification. The coagulant floc and the zeolite-like imidazolate framework-horseradish peroxidase composite material dispersed in the coagulant floc, the zeolite-like imidazolate framework-horseradish peroxidase includes the zeolite-like imidazolate framework matrix and attachment Horseradish peroxidase on the substrate; the preparation method includes adding the zeolite imidazolate skeleton-horseradish peroxidase composite material to the solution of the coagulant floc, mixing it on the surface of the ultrafiltration membrane Obtained after filtering. In the present invention, the composite material and hydroxyl iron flocs are co-deposited on the surface of the membrane, which can increase its sedimentation performance, effectively exert its pollution purification function and avoid hardening and passivation; adding bovine serum albumin can improve the diffusivity of horseradish peroxidase and utilization efficiency; the method is simple and feasible, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of water treatment and relates to ultrafiltration membrane water purification technology, in particular to zeolite imidazolate skeleton-horseradish peroxidase biomineralization embedding pre-coating and its preparation and its application in ultrafiltration membrane water purification application. Background technique [0002] In recent years, membrane separation technology represented by ultrafiltration has been popularized and applied on a large scale in water treatment, and has received high attention in the process of exploring new principles, new methods and new processes based on membrane separation. However, the main functions of ultrafiltration are filtration and solid-liquid separation, and it is difficult to effectively remove dissolved pollutants such as organic matter, heavy metals, and synthetic chemicals in water. [0003] There are often high concentrations of dissolved organic matter, low do...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F9/14B01D69/02
CPCB01D69/02C02F1/444C02F1/722C02F1/725C02F3/342
Inventor 孙丽华丁宇冯萃敏刘烨辉高呈史鹏飞
Owner 清上(苏州)环境科技有限公司
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