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A kind of deer bone protein extract and its preparation method and application

A protein extract and deer bone technology, applied in the field of biomedicine, can solve the problems of rare research on deer bone

Active Publication Date: 2022-03-08
EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is a big country for raising deer. Deer bone is rich in collagen, accounting for about 57%. However, deer bone is a high-quality resource of collagen, but there are few studies

Method used

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  • A kind of deer bone protein extract and its preparation method and application
  • A kind of deer bone protein extract and its preparation method and application
  • A kind of deer bone protein extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1, acid extraction and enzyme extraction method to extract protein extract from deer bone (red deer, sika deer)

[0064] 1. Preparation of Red Deer Bone Protein Extract

[0065] 1. Pretreatment

[0066] Weigh an appropriate amount of red deer leg bones, clean and remove the skin, tendons, and periosteum to obtain the bone, cut off the spongy bone at both ends of the bone, remove the bone marrow in the bone, rinse the bone with running water until there is almost no oil slick in the water, dry it, and crush it into bone Block (2cm×2cm), wash until the floating oil in the bone is removed. The bone pieces were dried (55-65° C.) until dry, and the bones were obtained and weighed.

[0067] 2. Boiling

[0068] Take the dried bones, put them into a multifunctional extraction tank, add water to decoct, decoct twice (0.1Mpa, 100°C), each time for 2.5h, filter (100 mesh), collect the filter residue (bones), and dry ( 60-65°C).

[0069] 3. Decalcification

[0070] Pu...

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Abstract

The invention discloses a deer bone protein extract, a preparation method and application thereof. The deer bone protein extract is a protein extract extracted from red deer bone and sika deer bone by acid extraction and enzymatic extraction, which can play the role of repairing, anti-wrinkle and anti-aging in the following aspects: (1) at the cell level : ① Improve the proliferation activity and renewal ability of skin keratinocytes, ② Improve the proliferation activity of skin fibroblasts, repair the decreased proliferation ability of skin fibroblasts caused by oxidative stress damage, thereby promoting the synthesis of endogenous collagen (2) Overall level: Exogenous deer bone protein extract can be transdermally absorbed into the dermis and integrated into its own collagen fibers, thus directly supplementing the collagen lost by aging skin.

Description

technical field [0001] The invention relates to a deer bone protein extract and its preparation method and application in the field of biomedicine. Background technique [0002] The skin, the largest organ in the body, consists of two distinct main components, the continuously renewing epidermis composed of keratinocytes and the dermis with a rich matrix composed of fibroblasts. Due to natural aging and the accumulation of external environmental factors (especially ultraviolet rays), the cell structure and function of skin tissue change, often causing skin aging damage such as sagging and wrinkles. Skin aging is reflected at the cellular level as cell aging, and the decrease in proliferation ability is the most direct manifestation of cell aging. [0003] Collagen is synthesized by fibroblasts in the connective tissue of the dermis and is the main structural protein in the extracellular matrix. Collagen exists in the form of collagen fibers in the skin, which is the suppor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30C07K14/78A61K8/98A61K8/65A61Q19/08A61K38/01A61P39/00A61P17/00
CPCC12P21/06C07K14/78A61K8/981A61K8/65A61Q19/08A61K38/014A61P39/00A61P17/00A61K2800/805
Inventor 王毅马淑骅孙娅楠杨伟峰李超华
Owner EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
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