Porcine circovirus 3 Cap protein, nucleic acid, virus-like particles, vaccine, and preparation method and application of porcine circovirus 3 Cap protein

Abstract

The invention provides a porcine circovirus 3 Cap protein, nucleic acid, virus-like particles, vaccine, and a preparation method and application of the porcine circovirus 3 Cap protein, and relates tothe technical field of molecular biology. The porcine circovirus 3 Cap protein provided by the invention has an amino acid sequence shown as SEQ ID NO.2, can be expressed in a soluble manner in a yeast expression system, and effectively improves yeast expression efficiency of the porcine circovirus 3 Cap protein. In addition, the porcine circovirus 3 Cap protein provided by the invention can be self-assembled into the virus-like particles, the vaccine prepared by using the virus-like particles has the characteristics of cell immunity and humoral immunity, and immunologic related experiments show that the immune effect is good. The method for expressing the porcine circovirus type 3 Cap protein by utilizing the yeast expression system has the advantages that production cost is low, the production process is simple, and a large amount of target proteins can be obtained through high-density fermentation, has no endotoxin pollution, and can be used for large-scale production.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a porcine circovirus type 3 Cap protein, a nucleic acid, a virus-like particle, a vaccine, a preparation method and an application. Background technique [0002] Porcine circovirus (PCV) is a single-stranded circular DNA virus with a gene length of about 1.7kb. It is one of the smallest animal DNA viruses. It mainly causes multisystem wasting syndrome, pneumonia, dermatitis, nephrotic syndrome, and reproductive impairment in piglets. , Bring heavy losses to the breeding of live pigs. PCV3 is a new type of circovirus, and there is no commercial vaccine against PCV3 in China. The homology of PCV3 and PCV2 cap protein genes is very low, only 30% identity, which also implies that the existing PCV2 vaccines cannot protect pigs from PCV3. Therefore, the research and development of PCV3 corresponding vaccine is very important. [0003] Recent studies have shown that PCV cap...

AI Technical Summary

Problems solved by technology

However, the PCVcap protein expressed by Escherichia coli has low solubility and is polluted by endotoxin, and the endotoxin removal process in the later stage is complex and expensive; the VLP expressed by the insect baculovirus system has a good immune effect, but the insect baculovirus produced VLP expression is low, production cost is expensive, the production process is complicated, and it is difficult to produce large-scale production as an animal vaccine

As an efficient protein expression system, the yeast expression system has many advantages, but due to the influence of the PCV3 cap protein's own nuclear localization sequence, it is easy to form insoluble protein aggregation when directly expressing the protein, so the soluble expression of the PCV3 cap protein in the yeast system challenging

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