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A detection kit and method for dicofol

A technology for detecting dicofol and a detection method, which is applied in the field of kits for detecting dicofol, can solve the problems of cumbersome steps, high detection limit of chemical chromogenic method, and high detection cost, so as to simplify the operation process, improve detection efficiency, and save operation steps Effect

Active Publication Date: 2021-02-09
广州食源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method adopts bipyridine chromogenic method, which requires the use of strong acid, and the detection limit cannot meet the limit requirements of tea, fruits and vegetables, and there are defects in use
[0007] In summary, it can be found that among common techniques, gas chromatography and high performance liquid chromatography have complex pretreatment processes, cumbersome steps, and high detection costs, which are not suitable for on-site detection; chemical chromogenic methods have high detection limits and cannot meet the requirements of GB2763- According to the limit requirements in 2019, the detection process requires strong acid decolorization, a large amount of organic solvents are used, and the chromogenic agent pyridine or pyridine derivatives are volatile and odorous toxic solvents, making on-site detection difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Weigh 3g of crushed tea leaves into a centrifuge tube, add 10mL of n-hexane, cover tightly, shake up and down 50 times, and let stand;

[0035] 2) Pour the extract into another centrifuge tube, add 0.5g of silicon-magnesium-type adsorbent, shake it 50 times, and let it stand for stratification;

[0036] 3) Take the supernatant and dry it, add 2mL pH8.0 buffer to redissolve, then add 0.5mL lipase, and pre-react for 5min;

[0037] 4) Add 0.5 mL of 0.15 mg / L p-nitrophenol ester solution, react for 10 min, and detect the absorbance at a wavelength of 405 nm.

Embodiment 2

[0039] 1) Weigh 5g of crushed vegetables into a centrifuge tube, add 8mL of acetone, cover tightly, shake up and down 50 times, and let stand;

[0040] 2) Pour the extract into another centrifuge tube, add 1g of silica gel, shake 50 times, and let stand to separate layers;

[0041] 3) Take the supernatant and dry it, add 1mL pH8.5 buffer to redissolve, then add 0.5mL lipase, and pre-react for 5min;

[0042] 4) Add 1.5 mL of 0.05 mg / L p-nitrophenol ester solution, react for 10 min, and detect the absorbance at a wavelength of 405 nm.

Embodiment 3

[0044] 1) Weigh 10 g of crushed fruit into a centrifuge tube, add 5 mL of ethyl acetate, cover tightly, shake up and down 50 times, and let stand;

[0045] 2) Pour the extract into another centrifuge tube, add 0.2g of neutral alumina, shake 50 times, and let stand to separate layers;

[0046] 3) Take the supernatant and dry it, add 4mL pH7.5 buffer solution to redissolve, then add lipase and nitrophenol ester solution, and detect the absorbance value at a wavelength of 405nm after reaction.

[0047] 1. Detection limit test:

[0048] Get 6 centrifuge tubes, add 5g of pulverized vegetable samples to each, add dicofol pesticide standard substance, the concentration of adding standard is respectively 0, 0.1, 0.2, 0.3, 0.4, 0.5mg / kg, according to the pretreatment method of embodiment 2 of the present invention and Detection method, the reaction solution detects the absorbance value at a wavelength of 405nm, and the following table is obtained:

[0049] Dicofol (mg / kg) 0...

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Abstract

The invention discloses a detection kit and a method for dicofol. The principle that the activity of lipase can be increased along with the increase of the concentration of the dicofol is utilized; whether the dicofol exists or not is detected by detecting the lipase activity, so that the dosage of an organic solvent is reduced, the detection limit is improved, strong acid and an organic solvent with foul smell are prevented from being used, the operation process is simplified, the detection efficiency is improved, and the method is suitable for field operation and rapid detection.

Description

technical field [0001] The invention relates to the field of organic compound detection, in particular to a kit and method for detecting dicofol. Background technique [0002] Dicofol is a low toxicity and high efficiency organochlorine acaricide, insoluble in water, soluble in various organic solvents, and has low toxicity to humans and animals. This kind of pesticide is a special pesticide for cicada mite pests. It has a good control effect on adults, nymphs and eggs. In addition, its structure is stable, its decomposition is slow, and its residual effect is long. Therefore, it was popularized and applied in my country's tea production in the late last century. . However, the production of most dicofol products uses DDT as the main intermediate, and the residual refining process is not up to standard, resulting in excessive DDT content in a large number of finished products. Therefore, when dicofol is sprayed, a certain amount of DDT is also mixed, so in the tea gardens a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/44
CPCC12Q1/44G01N2333/92
Inventor 谢俊平赖惠淸庄俊钰叶秋雄伍志权
Owner 广州食源生物科技有限公司
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